LOCUS       sequence-418819-        2895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In mammalian cells expresses a Quartet of receptor-binding domains 
            (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and 
            SARS-CoV-2 each separated by GS-linkers with a C-terminal SpyTag 
            fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Hills RA, Tan TK, Cohen AA, Keeffe JR, Keeble AH, Gnanapragasam PNP,
            Storm KN, Rorick AV, West AP Jr, Hill ML, Liu S, Gilbert-Jaramillo 
            J, Afzal M, Napier A, Admans G, James WS, Bjorkman PJ, Townsend AR, 
            Howarth MR
  TITLE     Proactive vaccination using multiviral Quartet Nanocages to elicit 
            broad anti-coronavirus responses.
  JOURNAL   Nat Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9.
  PUBMED    38710880
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9."
COMMENT     Sequence Label: Quartet-SpyTag
FEATURES             Location/Qualifiers
     source          1..2895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..108
                     /label=SnoopTag2
     CDS             complement(2471..2488)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             2764..2832
                     /codon_start=1
                     /product="peptide derived from the RrgA adhesin of 
                     Streptococcus pneumoniae"
                     /label=DogTag
                     /note="forms a covalent bond with the DogCatcher protein 
                     when inserted into an intra-domain protein loop (Keeble et 
                     al., 2022)"
                     /translation="DIPATYEFTDGKHYITNEPLPPK"
     CDS             2851..2889
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
ORIGIN
        1 atgaacaccc agatcctggt gttcgccctg atcgccatca ttcccaccaa cgccgacaag
       61 atcggatctg gaaaactggg ctatattgaa ttttataaag tggaaaaagg cagtggtgag
      121 tcgggatccg gaagagtggc ccctagcaaa gaagtcgtgc ggttccccaa tatcaccaat
      181 ctgtgcccct tcggcgaggt gttcaacgcc accacatttc cctctgtgta cgcctgggag
      241 agaaagcgga tcagcaactg cgtggccgac tacagcgtgc tgtacaacag cacctccttt
      301 agcaccttca agtgctacgg cgtgtccgcc accaagctga acgatctgtg cttctccaac
      361 gtgtacgccg acagcttcgt ggtcaagggc gacgatgtgc ggcagattgc tcctggacag
      421 acaggcgtga tcgccgatta caactacaag ctgcctgacg acttcctggg ctgtgtgctg
      481 gcctggaaca ccaatagcaa ggacagctcc accagcggca actacaacta cctgtacaga
      541 tgggtccgac gcagcaagct gaatccctac gagagggacc tgagcaacga catctatagc
      601 cctggcggcc agagctgttc tgccgtggga cccaattgct acaaccctct gaggccctac
      661 ggcttcttta ccacagctgg cgtgggccac cagccttaca gagtggtggt gctgtcattc
      721 gagctgctga atgcccctgc cacagtgtgt ggccctaagc tgtctaccga cctgatcaag
      781 aaccagggga gtggtgggtc cgggggttcg ggacgggtgt cccctacaca cgaggtcgtg
      841 cggttcccca atatcaccaa cagatgcccc ttcgacaagg tgttcaacgc cagcagattc
      901 cccaacgtgt acgcctggga gcgcaccaag atcagcgatt gcgtggccga ttacaccgtg
      961 ctgtacaact ccaccagctt tagcaccttt aagtgctacg gcgtgtcccc aagcaagctg
     1021 atcgacctgt gcttcacctc tgtgtacgcc gacaccttcc tgatccggtc tagcgaagtg
     1081 cgacaagtgg cccctggcga aacaggcgtg atcgccgact acaactacaa gctgcccgac
     1141 gacttcaccg gctgtgtgat cgcctggaat accgccaagc aggaccaggg ccagtactac
     1201 tacagaagca gcagaaagac caagctcaag cccttcgagc gggacctgac ctccgatgag
     1261 aatggcgtca gaaccctgtc cacctacgat ttctacccca atgtgcccat cgagtaccag
     1321 gccaccagag tggtggtgct gtctttcgaa ctgctgaacg cccctgccac agtgtgtggc
     1381 cctaaactgt ctacagccct ggtcaagaac cagtgcgtga acttcggcag cggcggaacg
     1441 ggaggtagtg gccgggtgca gcccaccgat agcatcgtgc ggttccccaa tatcaccaat
     1501 ctgtgcccct tcggcgaggt gttcaatgcc accacattcg cctctgtgta cgcctggaac
     1561 cggaagcgga tcagcaattg cgtggccgac tactccgtgc tgtacaacag caccagcttc
     1621 agcaccttca agtgctacgg cgtgtcccct accaagctga acgacctgtg cttcacaaac
     1681 gtgtacgccg acagcttcgt gatcaccggg gatgaagtgc ggcagattgc ccctggacag
     1741 acaggcaaga tcgccgacta caactacaag ctgcccgacg acttcaccgg ctgtgtgatt
     1801 gcctggaact ccaagcacat cgacgccaaa gaaggcggga acttcaacta cctgtaccgg
     1861 ctgttccgga aggccaacct gaagcctttc gagcgggata tctccaccga gatctaccag
     1921 gccggcagca agccttgtaa tggccagaca ggcctgaact gctactaccc actgtacaga
     1981 tacggcttct accccacaga cggcgtgggc caccagcctt atagagtggt ggtgctgagc
     2041 ttcgagctgc tgaatgcccc tgccacagtg tgcggcccta agaaaagcac caatctcgtg
     2101 aagaacaaat gcgtgaactt cggtacgggt ggcagtggag ggagcgggaa catcaccaat
     2161 ctgtgcccct tcggcgaggt gttcaatgcc acaagatttg ccagcgtgta cgcctggaac
     2221 cggaagagaa tcagcaactg cgtggccgac tacagcgtgc tgtacaatag cgccagcttc
     2281 agcaccttca agtgctacgg cgtgtcccct accaagctga acgacctgtg cttcaccaat
     2341 gtgtacgccg acagcttcgt gatcagaggc gacgaagttc ggcagatcgc tcctggacag
     2401 acaggcaaga tcgccgatta caactacaag ctgcccgacg acttcaccgg ctgcgtgatc
     2461 gcctggaata gcaacaacct ggacagcaaa gtcggcggca actacaacta cctgtaccgg
     2521 ctgttccgga agtccaacct gaagcctttc gagcgggaca tcagcaccga gatctatcag
     2581 gccggcagca ccccttgtaa tggcgtggaa ggcttcaact gctacttccc actgcagtcc
     2641 tacggcttcc agcctacaaa tggcgtcggc taccagcctt acagagtggt ggtgctgtcc
     2701 ttcgaactgc tgcatgcccc tgctaccgtg tgcggcccta aaaaaaccgg aggaggtggc
     2761 ggtgatattc cggctacata cgaattcacc gatggtaaac attatatcac caatgaaccg
     2821 ctgccgccga aaggtggtgg cggaggcgga gcccacatcg tgatggttga tgcctacaag
     2881 cccaccaaat agtga
//
