LOCUS       sequence-418883-        7008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ
  TITLE     A critical function for beta-amyloid precursor protein in neuronal 
            migration revealed by in utero RNA interference.
  JOURNAL   J Neurosci. 2007 Dec 26. 27(52):14459-69.
  PUBMED    18160654
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2007 Dec 26. 27(52):14459-69."
COMMENT     Sequence Label: pCAX APP 751
FEATURES             Location/Qualifiers
     source          1..7008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(6..110)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        141..520
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        522..799
                     /label=chicken beta-actin promoter
     intron          800..1816
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1740..1762
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1824..1843
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             complement(2219..2230)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             2710..2730
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(4166..4185)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4231..4286
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4285..4304)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4647..4663)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4647..4663)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4660..4682)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4671..4687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4695..4701,4702..4719,4720..4725))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4740..4761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4820..5015
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4866..5001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5021..5155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5058..5077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5112..5131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5223..5240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5394..5982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5474..5493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6153..6944,6945..7008,1..5))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6776..6795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
       61 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
      121 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
      181 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      241 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      301 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      361 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      421 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      481 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
      541 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
      601 ttaattattt tgtgcagcga tgggggcggg gggggggggg ggggcgggcc agtcgggggg
      661 gggggggggg gggggggggg ggggggcgag gcggagaggt gcggcggcag ccaatcagag
      721 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
      781 gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc
      841 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
      901 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
      961 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     1021 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     1081 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     1141 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     1201 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     1261 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     1321 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     1381 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg
     1441 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     1501 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     1561 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     1621 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     1681 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     1741 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     1801 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     1861 caaagaattc aggtaccgat atcctcgagc catgctgccc ggtttggcac tgctcctgct
     1921 ggccgcctgg acggctcggg cgctggaggt acccactgat ggtaatgctg gcctgctggc
     1981 tgaaccccag attgccatgt tctgtggcag actgaacatg cacatgaatg tccagaatgg
     2041 gaagtgggat tcagatccat cagggaccaa aacctgcatt gataccaagg aaggcatcct
     2101 gcagtattgc caagaagtct accctgaact gcagatcacc aatgtggtag aagccaacca
     2161 accagtgacc atccagaact ggtgcaagcg gggccgcaag cagtgcaaga cccatcccca
     2221 ctttgtgatt ccctaccgct gcttagttgg tgagtttgta agtgatgccc ttctcgttcc
     2281 tgacaagtgc aaattcttac accaggagag gatggatgtt tgcgaaactc atcttcactg
     2341 gcacaccgtc gccaaagaga catgcagtga gaagagtacc aacttgcatg actacggcat
     2401 gttgctgccc tgcggaattg acaagttccg aggggtagag tttgtgtgtt gcccactggc
     2461 tgaagaaagt gacaatgtgg attctgctga tgcggaggag gatgactcgg atgtctggtg
     2521 gggcggagca gacacagact atgcagatgg gagtgaagac aaagtagtag aagtagcaga
     2581 ggaggaagaa gtggctgagg tggaagaaga agaagccgat gatgacgagg acgatgagga
     2641 tggtgatgag gtagaggaag aggctgagga accctacgaa gaagccacag agagaaccac
     2701 cagcattgcc accaccacca ccaccaccac agagtctgtg gaagaggtgg ttcgagaggt
     2761 gtgctctgaa caagccgaga cggggccgtg ccgagcaatg atctcccgct ggtactttga
     2821 tgtgactgaa gggaagtgtg ccccattctt ttacggcgga tgtggcggca accggaacaa
     2881 ctttgacaca gaagagtact gcatggccgt gtgtggcagc gccattccta caacagcagc
     2941 cagtacccct gatgccgttg acaagtatct cgagacacct ggggatgaga atgaacatgc
     3001 ccatttccag aaagccaaag agaggcttga ggccaagcac cgagagagaa tgtcccaggt
     3061 catgagagaa tgggaagagg cagaacgtca agcaaagaac ttgcctaaag ctgataagaa
     3121 ggcagttatc cagcatttcc aggagaaagt ggaatctttg gaacaggaag cagccaacga
     3181 gagacagcag ctggtggaga cacacatggc cagagtggaa gccatgctca atgaccgccg
     3241 ccgcctggcc ctggagaact acatcaccgc tctgcaggct gttcctcctc ggcctcgtca
     3301 cgtgttcaat atgctaaaga agtatgtccg cgcagaacag aaggacagac agcacaccct
     3361 aaagcatttc gagcatgtgc gcatggtgga tcccaagaaa gccgctcaga tccggtccca
     3421 ggttatgaca cacctccgtg tgatttatga gcgcatgaat cagtctctct ccctgctcta
     3481 caacgtgcct gcagtggccg aggagattca ggatgaagtt gatgagctgc ttcagaaaga
     3541 gcaaaactat tcagatgacg tcttggccaa catgattagt gaaccaagga tcagttacgg
     3601 aaacgatgct ctcatgccat ctttgaccga aacgaaaacc accgtggagc tccttcccgt
     3661 gaatggagag ttcagcctgg acgatctcca gccgtggcat tcttttgggg ctgactctgt
     3721 gccagccaac acagaaaacg aagttgagcc tgttgatgcc cgccctgctg ccgaccgagg
     3781 actgaccact cgaccaggtt ctgggttgac aaatatcaag acggaggaga tctctgaagt
     3841 gaagatggat gcagaattcc gacatgactc aggatatgaa gttcatcatc aaaaattggt
     3901 gttctttgca gaagatgtgg gttcaaacaa aggtgcaatc attggactca tggtgggcgg
     3961 tgttgtcata gcgacagtga tcgtcatcac cttggtgatg ctgaagaaga aacagtacac
     4021 atccattcat catggtgtgg tggaggttga cgccgctgtc accccagagg agcgccacct
     4081 gtccaagatg cagcagaacg gctacgaaaa tccaacctac aagttctttg agcagatgca
     4141 gaactagagc ggccgcctcg agagatcttt ttccctctgc caaaaattat ggggacatca
     4201 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     4261 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     4321 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     4381 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     4441 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     4501 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     4561 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
     4621 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     4681 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4741 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     4801 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     4861 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4921 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4981 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     5041 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5101 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     5161 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5221 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5281 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5341 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5401 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5461 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5521 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5581 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5641 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5701 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5761 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5821 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5881 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5941 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6001 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6061 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6121 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6181 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6241 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6301 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6361 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6421 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6481 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6541 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6601 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6661 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6721 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6781 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6841 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6901 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6961 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaata
//
