LOCUS       sequence-418923-        9791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses the reverse 
            tetracycline-controlled transactivator (rtTA) with hygromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Richner M, Victor MB, Liu Y, Abernathy D, Yoo AS
  TITLE     MicroRNA-based conversion of human fibroblasts into striatal medium 
            spiny neurons.
  JOURNAL   Nat Protoc. 2015 Oct;10(10):1543-55. doi: 10.1038/nprot.2015.102. 
            Epub 2015 Sep 17.
  PUBMED    26379228
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.102"; journalName: "Nat Protoc"; date: 
            "2015-10"; volume: "10"; issue: "10"; pages: "1543-55"
COMMENT     Sequence Label: rtTA-N144
FEATURES             Location/Qualifiers
     source          1..9791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..141
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(404..448)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    633..866
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1363..1488
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1535..2168
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2336..2470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2360..2379)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2414..2433
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2518..2622
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2623..2691,2692..3483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2841..2860)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3654..4242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4143..4162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4396..4413
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4530..4551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4566..4571,4572..4589,4590..4596)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4604..4620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4609..4631
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4628..4644
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4628..4644
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4772..5405
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5612..6200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5718..5729
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6127..6147
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6224..7249)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6224..7249)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7276..7775)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7324..7343)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7717..7740
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(join(7824..7832,7833..7868,7869..7871,7872..7907,
                     7908..7910,7911..7946,7947..7952,7953..8570))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     intron          8589..9527
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(8612..8632)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(9528..9739)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
       61 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
      121 aatcccccct tttcttttaa aggcgatacc gtcgagatcc gttcactaat cgaatggatc
      181 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg
      241 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta
      301 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata
      361 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat
      421 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg
      481 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat
      541 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc
      601 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt
      661 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat
      721 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata
      781 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
      841 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
      901 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
      961 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc
     1021 ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc
     1081 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt
     1141 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat
     1201 ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc
     1261 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg
     1321 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc
     1381 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc
     1441 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt
     1501 ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa
     1561 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     1621 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     1681 tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta
     1741 tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa
     1801 gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg
     1861 atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac
     1921 ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc
     1981 tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc
     2041 aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta
     2101 gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg agtgaattag
     2161 cccttccaac tactaagttt gtagtacata tttaacaaat acaatttctt taaaatgaaa
     2221 ataattcaga ggaatcacag gtttagagta aatgaaacca caggtaattg gcagtggtaa
     2281 tagggtatgg ggtgggaagt ttgggatgat tttggttagc ttgagttatc cagttgatcc
     2341 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2401 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2461 taaacaagtt gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc
     2521 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2581 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2641 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2701 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2761 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2821 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2881 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2941 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3001 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3061 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3121 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3181 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3241 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3301 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3361 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3421 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3481 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3541 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3601 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3661 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3721 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3781 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3841 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3901 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3961 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4021 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4081 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4141 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4201 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4261 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4321 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4381 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     4441 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     4501 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4561 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4621 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgacggt
     4681 aaacgctagc aatgtcaagg cctctcactc tctgatattc atttctttgc aagttataaa
     4741 tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag
     4801 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     4861 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     4921 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat
     4981 gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc ctggaaagtc
     5041 cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt actccggatg
     5101 cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact ctaacacttc
     5161 tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg tgttctctcc
     5221 tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc accatccaaa
     5281 ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag ggaagtagcc
     5341 ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt cgttgggagt gaattagccc
     5401 ttccagtccc ctcttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     5461 tggtcttaaa ggtacctgag gtgtgactgg aaaacccacc tcctcctcct cttgtgcttc
     5521 tagccaggca caatcagcat tggtagctgc tgtattgcta cttgtgattg ctccatgttt
     5581 ttctaggtct cgactgcaga attaattcca ggcggggagg cggcccaaag ggagatccga
     5641 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     5701 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     5761 atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc cgacaacacc
     5821 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     5881 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     5941 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     6001 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     6061 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     6121 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     6181 ttgtaatcca gaggttgatt gttccagacg cgccccgccc gggctattcc tttgccctcg
     6241 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     6301 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     6361 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     6421 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     6481 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     6541 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     6601 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     6661 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     6721 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     6781 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     6841 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     6901 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     6961 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
     7021 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     7081 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     7141 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     7201 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcatg gtcctaggaa
     7261 gcttgggctg caggtcgaaa ggcccggaga tgaggaagag gagaacagcg cggcagacgt
     7321 gcgcttttga agcgtgcaga atgccgggcc tccggaggac cttcgggcgc ccgccccgcc
     7381 cctgagcccg cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa
     7441 agcgaaggag caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc
     7501 agcggtgctg tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct
     7561 gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg
     7621 cgcgaagggg ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt
     7681 gcgaggccag aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc
     7741 tccagactgc cttgggaaaa gcgcctcccc tacccggtag aattcacgcg ttcgcgatct
     7801 agagccggcg cggccgcgga tccttacccg gggagcatgt caaggtcaaa atcgtcaaga
     7861 gcgtcagcag gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct
     7921 aagtcaaaat cgtcaagggc gtcggccggc ccgccgcttt cgcactttag ctgtttctcc
     7981 aggccacata tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg
     8041 aacagctcaa ttgcttgtct cagaagtggg ggcatagaat cggtggtagg tgtctctctt
     8101 tcctcttttg ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc
     8161 acggcggaca gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg
     8221 agctgatttt ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta
     8281 gccccgtcgc gatgtgagag gagagcacag cggaatgact tggcgttgtt ccgcagaaag
     8341 tcttgccatg actcgccttc cagggggcag aagtgggtat gatgcctgtc cagcatctcg
     8401 attggcaggg catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc
     8461 tcaactccca gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gactccattg
     8521 agtaattcca gagcgccgtt tatgactttg ctcttgtcca gtctagacat ggtggatcct
     8581 cacgacacct gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac
     8641 caagatccaa actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc
     8701 ctaacttcag tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc
     8761 caacctaaag acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc
     8821 cggtactccg tggagtcaca tgaagcgacg gctgaggacg gaaaggccct tttcctttgt
     8881 gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc tccattttga gctccctgca
     8941 gcagggccgg gaagcggcca tctttccgct cacgcaactg gtgccgaccg ggccagcctt
     9001 gccgcccagg gcggggcgat acacggcggc gcgaggccag gcaccagagc aggccggcca
     9061 gcttgagact acccccgtcc gattctcggt ggccgcgctc gcaggccccg cctcgccgaa
     9121 catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca
     9181 gtgtgcagat cttggcccgc atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca
     9241 ggtcatcaaa aattttaaat ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg
     9301 tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac tcaagcacga
     9361 ggcgaagggg ctccttaagc gcaaggcctc gaactctccc acccacttcc aacccgaagc
     9421 tcgggatcaa gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca
     9481 taacccgtaa agaggccagg cccgcgggaa ccacacacgg cacttacctg tgttctggcg
     9541 gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc
     9601 ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc
     9661 caccttctct aggcaccggt tcaattgccg acccctcccc ccaacttctc ggggactgtg
     9721 ggcgatgtgc gctctgccca ctgacgggca cctcgagtga agacgaaagg gcctcatcga
     9781 taaactggat c
//
