LOCUS       sequence-419117-        1690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EC1000 containing pORI28. Used as the source of plasmid pORI28. With
            pTRK669, part of a system for chromosomal integration in gram 
            positive bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Leenhouts K, Buist G, Bolhuis A, ten Berge A, Kiel J, Mierau I, 
            Dabrowska M, Venema G, Kok J
  TITLE     A general system for generating unlabelled gene replacements in 
            bacterial chromosomes.
  JOURNAL   Mol Gen Genet. 1996 Nov 27;253(1-2):217-24.
  PUBMED    9003306
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Gen 
            Genet."; date: "1996-11-27"; volume: "253(1-2)"; pages: "217-24"
COMMENT     Sequence Label: pORI28
FEATURES             Location/Qualifiers
     source          1..1690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             194..205
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cactcggcac ttaatggggg gtcgtagtac ggaagcaaaa ttcgcttcct ttccccccat
       61 ttttttccaa attccaaatt tttttcaaaa attttccagc gctaccgctc ggcaaaattg
      121 caagcaattt ttaaaatcaa acccatgagg gaatttcatt ccctcatact cccttgagcc
      181 tcctccaacc gaaatagaag ggcgctgcgc ttattatttc attcagtcat cggctttcat
      241 aatctaacag acaacatctt cgctgcaaag ccacgctacg ctcaagggct tttacgctac
      301 gataacgcct gttttaacga ttatgccgat aactaaacga aataaacgct aaaacgtctc
      361 agaaacgatt ttgagacgtt ttaataaaaa atcgctagtc cgaggcctcg acccgattca
      421 caaaaaatag gcacacgaaa aacaagttaa gggatgcagt ttatgcatcc cttaacttac
      481 ttattaaata atttatagct attgaaaaga gataagaatt gttcaaagct aatattgttt
      541 aaatcgtcaa ttcctgcatg ttttaaggaa ttgttaaatt gattttttgt aaatattttc
      601 ttgtattctt tgttaaccca tttcataacg aaataattat acttttgttt atctttgtgt
      661 gatattcttg atttttttct acttaatctg ataagtgagc tattcacttt aggtttagga
      721 tgaaaatatt ctcttggaac catacttaat atagaaatat caacttctgc cattaaaagt
      781 aatgccaatg agcgttttgt atttaataat cttttagcaa acccgtattc cacgattaaa
      841 taaatctcat tagctatact atcaaaaaca attttgcgta ttatatccgt acttatgtta
      901 taaggtatat taccatatat tttataggat tggtttttag gaaatttaaa ctgcaatata
      961 tccttgttta aaacttggaa attatcgtga tcaacaagtt tattttctgt agttttgcat
     1021 aatttatggt ctatttcaat ggcagttacg aaattacacc tctttactaa ttcaagggta
     1081 aaatggcctt ttcctgagcc gatttcaaag atattatcat gttcatttaa tcttatattt
     1141 gtcattattt tatctatatt atgttttgaa gtaataaagt tttgactgtg ttttatattt
     1201 ttctcgttca ttataaccct ctttaatttg gttatatgaa ttttgcttat taacgattca
     1261 ttataaccac ttattttttg tttggttgat aatgaactgt gctgattaca aaaatactaa
     1321 aaatgcccat attttttcct ccttataaaa ttagtataat tatagcacgg tcgagatcta
     1381 tcgatgcatg ccatggtacc cgggagctcg aattcgaagc ttctgcagac gcgtcgacgt
     1441 catatggatc cgatatcgcc gtggcggccg ctctagaact agcgattctg aaatcaccat
     1501 ttaaaaaact ccaatcaaat aattttataa agttagtgta tcactttgta atcataaaaa
     1561 caacaataaa gctacttaaa tatagattta taaaaaacgt tggcgaaaac gttggcgatt
     1621 cgttggcgat tgaaaaaccc cttaaaccct tgagccagtt gggatagagc gtttttggca
     1681 caaaaattgg
//
