LOCUS       sequence-419260-       10694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human TopBP1 C-terminally fused to mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10694)
  TITLE     Pospiech Lab Plasmids 2023
REFERENCE   2  (bases 1 to 10694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-TopBP1-mVen
FEATURES             Location/Qualifiers
     source          1..10694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(81..100)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        272..651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        652..855
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     806..826
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     900..919
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        900..918
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(4572..4583)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      5549..5558
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5555..5557,5558..5560,5561..6274)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5600..5621)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5861..5880)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6208..6229
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(6284..6302)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6285..6302)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6322..6339)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6328..6552
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6598..7026
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6685..6704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6895..6916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7035..7055)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7040..7369
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7220..7355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7282..7301
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7436..8230
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7490..7509)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8100..8119
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8404..8525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8441..8460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8495..8514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8574..8590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8574..8590)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8587..8609)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8598..8614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8622..8628,8629..8646,8647..8652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8667..8688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8805..8822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8976..9564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9056..9075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9735..10526,10527..10595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10358..10377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10596..10694,1..6))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
       61 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
      121 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
      181 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
      241 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      301 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      361 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      421 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      481 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      541 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      601 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      661 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      721 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      781 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      841 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
      901 aatacgactc actataggga gacccaagct tggtaccgag ctcggatcca tgtccagaaa
      961 tgacaaagaa ccgttttttg tgaagttttt aaagtcttca gacaattcca aatgtttttt
     1021 taaagctctc gagtccataa aagaattcca atcagaagaa tatcttcaga ttattacaga
     1081 agaagaggca ttgaagataa aggagaatga tagatcactt tatatctgtg acccttttag
     1141 tggcgttgtc tttgatcacc tcaaaaagct tggctgcaga attgttggtc ctcaagtagt
     1201 catattttgt atgcaccacc agcgatgtgt cccaagagcc gaacatccag tttataatat
     1261 ggttatgtct gatgtaacca tatcttgtac aagtctggaa aaagaaaaaa gggaagaagt
     1321 tcataaatat gtacaaatga tgggcggacg agtatacaga gaccttaatg tatcagtaac
     1381 tcaccttatt gcaggagaag ttggtagcaa aaaatattta gttgctgcaa acctgaagaa
     1441 acctattttg cttccctctt ggataaaaac actttgggag aagtcacaag agaaaaaaat
     1501 aactagatat actgatataa acatggaaga tttcaagtgt cctatttttc ttggttgcat
     1561 aatctgtgtg actggcttat gtggcttaga caggaaagaa gttcagcaac tcacagttaa
     1621 gcatggaggt caatacatgg gacaattgaa aatgaatgaa tgtacacacc tcattgtgca
     1681 agaaccaaaa ggtcagaagt atgagtgtgc caagagatgg aatgtacact gtgtgaccac
     1741 acagtggttt tttgacagta ttgagaaagg tttttgtcag gatgaatcca tatacaagac
     1801 agaacctaga ccagaagcaa agactatgcc caattcttca actcctacca gccagatcaa
     1861 cacaattgat agtcgtactc tttcagatgt cagcaatatt tccaacataa atgcaagttg
     1921 cgtaagtgaa tcaatatgta attcacttaa cagcaaactg gagcctacac ttgaaaatct
     1981 agaaaatctg gatgtcagtg catttcaagc acctgaagat ttattagatg gttgtcggat
     2041 atatctttgc ggttttagtg gcagaaagct agataaactg agaagactta ttaacagtgg
     2101 aggtggagtt cgttttaacc agctaaatga agatgtaact catgttattg tgggagatta
     2161 tgatgatgaa ttgaagcagt tttggaataa atcagcccac aggcctcatg tagtgggagc
     2221 aaagtggttg ctagagtgtt tcagtaaagg ttatatgctt tctgaagaac catatatcca
     2281 tgctaattac cagccagtgg aaattccagt ttcacatcag cctgaaagta aagcagctct
     2341 tttaaaaaag aagaacagca gcttctctaa gaaagacttt gctcctagtg aaaagcatga
     2401 gcaagctgat gaagatctgc tctctcaata tgaaaatggt agctccacag tagttgaggc
     2461 taagacgtct gaagccaggc cctttaatga ttctactcat gctgagccct tgaatgattc
     2521 tactcacatt tctttgcaag aagaaaacca gtcttctgtc agtcattgtg tccctgatgt
     2581 ttctacaatt actgaagaag gcttatttag ccaaaagagt ttccttgttt tgggttttag
     2641 taatgaaaat gaatctaaca tcgcaaacat cataaaagaa aatgctggga aaatcatgtc
     2701 ccttctgagc agaactgttg cggattatgc tgtggttcct ctgctggggt gtgaagtgga
     2761 agccactgtg ggagaagttg ttacaaatac atggctggtt acttgcatag actatcagac
     2821 tttgtttgat ccaaagtcga atcctctctt cacaccagtt ccagtaatga caggaatgac
     2881 tcctttagag gattgtgtta tttcatttag ccagtgtgct ggagcagaaa aagagtcttt
     2941 aactttccta gcaaacctcc ttggagcaag tgttcaagaa tactttgttc gcaaatccaa
     3001 tgcaaagaaa ggcatgtttg ccagtactca tcttatactg aaagaacgtg gtggctctaa
     3061 atatgaagct gcaaagaagt ggaatttacc tgccgttact atagcttggc tgttggagac
     3121 tgctagaacg ggaaagagag cagacgaaag ccattttctg attgaaaatt caactaaaga
     3181 agaacgaagt ttggaaacag aaataacaaa tggaatcaat ctaaattcag atactgcaga
     3241 gcatcctggc acacgcctgc aaactcacag aaaaaccgtc gttacacctt tagatatgaa
     3301 ccgctttcag agtaaagctt tccgtgctgt ggtctcacaa catgccagac aggtcgcagc
     3361 ctccccagca gtaggacaac cacttcagaa ggagccctcg ttacacctgg atacaccatc
     3421 aaaattcctg tccaaggaca aactcttcaa gccttccttt gatgtgaagg atgcacttgc
     3481 agccttggaa actccaggac gtcccagcca acagaaaagg aaaccgagta cgccactctc
     3541 agaagttatt gtcaaaaact tgcaacttgc tttggcaaat agctctcgaa atgctgtcgc
     3601 tctttctgcc agccctcaac tgaaagaggc ccagtcagag aaggaagaag ccccaaagcc
     3661 acttcacaaa gtagtggtat gtgttagtaa aaaactcagt aagaagcaga gtgaactaaa
     3721 tgggatcgca gcctctctag gagcagatta caggtggagt tttgatgaaa cagtgactca
     3781 tttcatctat caagggcggc caaatgacac taatcgggag tataaatctg taaaagaaag
     3841 aggagtacac attgtttccg agcactggct tttagattgt gcccaagagt gtaaacatct
     3901 tcctgaatct ctttatccac atacttataa tcccaaaatg agcttggata tcagcgcagt
     3961 gcaagatggc cggctctgta atagtcgact actctcagct gtgtcttcaa caaaggatga
     4021 tgagccagat cctttgattt tagaagaaaa tgatgtagac aatatggcca ccaataataa
     4081 agagtcagca ccatcaaatg gaagtggaaa gaatgactct aaaggagttc tgacacagac
     4141 cttagagatg agagagaact ttcagaagca gttacaggag ataatgtctg caacatcaat
     4201 agtgaaaccc caagggcaga ggacttccct ttcaagaagt ggttgtaaca gcgcatcttc
     4261 aacccctgac agcactcgct ctgctcgcag tggacgaagt agagtcctag aggcactgag
     4321 gcagtctcgt cagacagtac ctgatgtcaa cacagagcct tcccaaaatg aacagatcat
     4381 ttgggatgac cctacagcaa gggaggagag agcaaggctt gccagcaatt tgcagtggcc
     4441 tagttgtccc acacaatact ctgagcttca ggttgacatt caaaacttgg aggattctcc
     4501 ttttcaaaag cctttacatg attcagaaat tgctaaacag gctgtctgtg atcctggaaa
     4561 catacgtgtg actgaagctc ccaaacaccc aatctctgaa gaactggaaa ctcccataaa
     4621 agacagccac ctgatcccta cgcctcaagc ccccagtatt gcctttccac tcgccaaccc
     4681 ccctgtggct ccgcacccta gagaaaagat tataacgata gaggagactc atgaagaatt
     4741 aaaaaaacag tacatatttc agttatcatc tctgaatcct caagaacgta ttgactattg
     4801 tcatctgatt gagaaactag gtggattggt gatagaaaag cagtgctttg atcccacctg
     4861 tacacacatt gttgtgggac atccacttcg aaacgagaag tatttagcct cagtggcagc
     4921 tgggaagtgg gtgcttcatc gctcctacct tgaagcctgc aggactgctg gacacttcgt
     4981 gcaggaagaa gactatgaat ggggaagtag ttccatactt gatgttctga ctggaatcaa
     5041 tgtacagcaa cgaagactag cacttgcagc aatgagatgg agaaaaaaaa tccagcaaag
     5101 acaagaatct ggcattgttg agggagcatt tagtgggtgg aaggttattt tacatgtgga
     5161 tcagtctcga gaagcaggct tcaaacgcct tcttcagtca ggaggagcaa aggtgctacc
     5221 tggtcattct gtacctttat ttaaagaggc cacacatctt ttttctgact tgaataaact
     5281 gaaaccagat gactcaggag ttaatatagc agaagctgct gcccagaacg tgtactgctt
     5341 gagaacagaa tacattgctg attatctcat gcaggaatca cctcctcatg tagaaaatta
     5401 ctgtctacca gaagctattt catttattca gaataataag gaacttggga ctggattatc
     5461 acaaaagagg aaagctccta cagaaaaaaa taaaatcaaa cgacctagag tacactcgcg
     5521 gccgctcgag catgcatcta gaccggtcgc caccatggtg agcaagggcg aggagctgtt
     5581 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     5641 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
     5701 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
     5761 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     5821 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     5881 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     5941 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     6001 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     6061 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     6121 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     6181 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     6241 gatcactctc ggcatggacg agctgtacaa gtaagggccc tattctatag tgtcacctaa
     6301 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6361 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6421 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6481 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6541 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     6601 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6661 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6721 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6781 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6841 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6901 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6961 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7021 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7081 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7141 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7201 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7261 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     7321 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     7381 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     7441 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     7501 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     7561 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     7621 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     7681 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     7741 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7801 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7861 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7921 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     7981 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     8041 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     8101 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     8161 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     8221 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     8281 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8341 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8401 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8461 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8521 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8581 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8641 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8701 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8761 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8821 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8881 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8941 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9001 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9061 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9121 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9181 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9241 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9301 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9361 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9421 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9481 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9541 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9601 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9661 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9721 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9781 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9841 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9901 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9961 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10021 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10081 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10141 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10201 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10261 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10321 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10381 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10441 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10501 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10561 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10621 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10681 aaacaaatag gggt
//
