LOCUS       sequence-419505-        9030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human AAVS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpur...
FEATURES             Location/Qualifiers
     source          1..9030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(48..503)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(185..206)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     397..416
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     630..652
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     644..661
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     645..661
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     671..690
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        671..689
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1005..1203
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1158..1178
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1221..1401
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1448..1573
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2072..2305
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2490..2534
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2832..2949
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3406..3654
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3406..3426
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3577..3596
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3781..4280
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3816..3839)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4213..4232
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4320..4916
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4320..4339)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4816..4836
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4935..4988
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4989..5690
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(5750..5770)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6168..6179)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6631..6648)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6637..6861
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6887..6907)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6887..6905)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6926..6942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6926..6942)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6939..6961)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6950..6966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6974..6980,6981..6998,6999..7004))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7019..7040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7157..7174)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7328..7916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7408..7427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8087..8878,8879..8947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8710..8729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8948..9030,1..22))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
       61 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
      121 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
      181 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
      241 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
      301 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
      361 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
      421 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
      481 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
      541 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      601 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      661 tgagcgcgcg taatacgact cactataggg cgaattgact agtacataac ttacggtaaa
      721 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      781 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      841 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      901 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      961 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     1021 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     1081 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     1141 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     1201 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     1261 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     1321 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     1381 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     1441 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     1501 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     1561 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     1621 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     1681 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     1741 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     1801 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     1861 agacaagata gaggaacagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
     1921 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1981 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     2041 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     2101 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     2161 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     2221 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     2281 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     2341 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     2401 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     2461 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     2521 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2581 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2641 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2701 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2761 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     2821 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2881 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2941 caaaattttc gggtttatta cagggacagc agagatccag tttggttagt accgggccct
     3001 acgcgtctgc agtatttagc atgccccacc catctgcaag gcattctgga tagtgtcaaa
     3061 acagccggaa atcaagtccg tttatctcaa actttagcat tttgggaata aatgatattt
     3121 gctatgctgg ttaaattaga ttttagttaa atttcctgct gaagctctag tacgataagc
     3181 aacttgacct aagtgtaaag ttgagacttc cttcaggttt atatagcttg tgcgccgctt
     3241 gggtacctcg gtccctagtg gccccactgg tttaagagct atgctggaaa cagcatagca
     3301 agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     3361 ttagccgaac tgtttcacac tcacgcgtcc aaggtcgggc aggaagaggg cctatttccc
     3421 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
     3481 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     3541 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     3601 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct
     3661 tcgagaagac acgtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag
     3721 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttggatc caattctacc
     3781 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     3841 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     3901 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     3961 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     4021 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     4081 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     4141 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     4201 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     4261 cctcatctcc gggcctttcg acctgcatcc atctagatct cgagcagctg aagcttacca
     4321 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     4381 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     4441 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     4501 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     4561 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     4621 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     4681 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     4741 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     4801 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     4861 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccggcg
     4921 gcgggtccgg aggagagggc agaggaagtc tcctaacatg cggtgacgtg gaggagaatc
     4981 ctggcccaat gagcgagctg attaaggaga acatgcacat gaagctgtac atggagggca
     5041 ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     5101 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     5161 tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg
     5221 acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgagg
     5281 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     5341 acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac
     5401 tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa
     5461 acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc aagaccacat
     5521 atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca
     5581 gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag
     5641 tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaattga gcggccgctc
     5701 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5761 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5821 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5881 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5941 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6001 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6061 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6121 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6181 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctacgcc
     6241 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcaggta cctttaagac
     6301 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6361 aagggctaat tcactcccaa agaagtcaag atctgctttt tgcctgtact gggtctctct
     6421 ggttagacca gagtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6481 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6541 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6601 aatctctagc agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6661 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6721 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6781 ggggtggggt ggggcaggac agcaaggggg aggattggga agtcaatagc aggcatgctg
     6841 gggatgcggt gggctctatg ggcggccgtt aattaagctt ttgttccctt tagtgagggt
     6901 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6961 tcacaattcc acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat
     7021 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7081 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7141 ggcgctgttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7201 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7261 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7321 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7381 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7441 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7501 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7561 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7621 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7681 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7741 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7801 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7861 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7921 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7981 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8041 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8101 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8161 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8221 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8281 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8341 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8401 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8461 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8521 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8581 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8641 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8701 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8761 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8821 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8881 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8941 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9001 gcggatacat atttgaatgt atttagaaaa
//
