LOCUS       sequence-419582-        3495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Renilla SV40 promoter (no enhancer) reporter gene for normalization 
            of luciferase experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Chen X, Prywes R
  TITLE     Serum-induced expression of the cdc25A gene by relief of 
            E2F-mediated repression.
  JOURNAL   Mol Cell Biol. 1999 Jul . 19(7):4695-702.
  PUBMED    10373518
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Jul . 19(7):4695-702."
COMMENT     Sequence Label: pRL-SV40P
FEATURES             Location/Qualifiers
     source          1..3495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1835..2031
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1882..2017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2101..2233
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2216..2235
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        2278..2296
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2306..3241
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    3272..3393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3283..3302)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3337..3356
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gatccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatggctcg acagatctgc gatctgcatc tcaattagtc agcaaccata
     1861 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     1921 ccccatcgct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     1981 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgat
     2041 tcttctgaca caacagtctc gaacttaagc tgcagaagtt ggtcgtgagg cactgggcag
     2101 gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga
     2161 cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc
     2221 tttctctcca caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa
     2281 tacgactcac tataggctag ccaccatgac ttcgaaagtt tatgatccag aacaaaggaa
     2341 acggatgata actggtccgc agtggtgggc cagatgtaaa caaatgaatg ttcttgattc
     2401 atttattaat tattatgatt cagaaaaaca tgcagaaaat gctgttattt ttttacatgg
     2461 taacgcggcc tcttcttatt tatggcgaca tgttgtgcca catattgagc cagtagcgcg
     2521 gtgtattata ccagacctta ttggtatggg caaatcaggc aaatctggta atggttctta
     2581 taggttactt gatcattaca aatatcttac tgcatggttt gaacttctta atttaccaaa
     2641 gaagatcatt tttgtcggcc atgattgggg tgcttgtttg gcatttcatt atagctatga
     2701 gcatcaagat aagatcaaag caatagttca cgctgaaagt gtagtagatg tgattgaatc
     2761 atgggatgaa tggcctgata ttgaagaaga tattgcgttg atcaaatctg aagaaggaga
     2821 aaaaatggtt ttggagaata acttcttcgt ggaaaccatg ttgccatcaa aaatcatgag
     2881 aaagttagaa ccagaagaat ttgcagcata tcttgaacca ttcaaagaga aaggtgaagt
     2941 tcgtcgtcca acattatcat ggcctcgtga aatcccgtta gtaaaaggtg gtaaacctga
     3001 cgttgtacaa attgttagga attataatgc ttatctacgt gcaagtgatg atttaccaaa
     3061 aatgtttatt gaatcggacc caggattctt ttccaatgct attgttgaag gtgccaagaa
     3121 gtttcctaat actgaatttg tcaaagtaaa aggtcttcat ttttcgcaag aagatgcacc
     3181 tgatgaaatg ggaaaatata tcaaatcgtt cgttgagcga gttctcaaaa atgaacaata
     3241 attctagagc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     3301 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     3361 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     3421 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     3481 ggtaaaatcg ataag
//
