LOCUS       sequence-419585-        9408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9408)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMV/TO SV40 small + Large T (w612-1)
FEATURES             Location/Qualifiers
     source          1..9408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(83..107)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    138..726
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(191..211)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(609..620)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             813..1046
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1118..1239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1155..1174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1209..1228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1279..1414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1434..1453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1435..1453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1463..1480)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1463..1479)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1472..1494)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1621..2076
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1708..1727)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1918..1939
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2102..2206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2207..2275,2276..3067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2425..2444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3238..3826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3727..3746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3980..3997
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4114..4135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4150..4155,4156..4173,4174..4180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4188..4204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4193..4215
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4212..4228
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4212..4228
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4247..4267
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4249..4267
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4295..4521
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4522..4702
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4749..4874
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5367..5600
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5785..5829
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6096..6212
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6235..6538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6539..6742
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6693..6713
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6744..6762
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6765..6783
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6824..6848
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6955..7200,7547..9408,1..19)
                     /codon_start=1
                     /product="SV40 (simian virus 40) large T antigen"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     intron          7201..7546
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
ORIGIN
        1 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt tggatccggt accgaattcg
       61 cggccgcact cgagatatct agacccagct ttcttgtaca aagtggttga tatccagcac
      121 agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
      181 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
      241 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
      301 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
      361 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
      421 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
      481 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
      541 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
      601 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
      661 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
      721 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
      781 tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag
      841 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
      901 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      961 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     1021 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     1081 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     1141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     1261 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     1321 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1381 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     1441 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1501 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1561 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     1621 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     1681 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     1741 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1801 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     1861 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     1921 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1981 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     2041 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     2101 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2161 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2221 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2281 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2341 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2401 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2461 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2521 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2581 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2641 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2701 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2761 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2821 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2881 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2941 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3001 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3061 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3121 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3181 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3241 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3301 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3361 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3421 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3481 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3541 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3601 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3661 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3721 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3781 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3841 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3901 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     4021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4081 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     4141 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     4201 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     4261 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     4321 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     4381 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     4441 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     4501 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     4561 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4621 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4681 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     4741 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     4801 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     4861 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     4921 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     4981 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     5041 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     5101 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     5161 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     5221 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     5281 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     5341 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     5401 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     5461 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     5521 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     5581 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     5641 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     5701 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     5761 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     5821 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     5881 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     5941 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     6001 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     6061 cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg
     6121 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     6181 ttacaaaaac aaattacaaa attcaaaatt ttatcgataa gcttgggagt tccgcgttac
     6241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     6301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     6361 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     6421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     6481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     6541 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     6601 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     6661 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     6721 ggaggtctat ataagcagag ctctccctat cagtgataga gatctcccta tcagtgatag
     6781 agatcgtcga ctagtccagt gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa
     6841 agcaggcttt aaaggaacca attcagtcga ctggatccgc ggcctctgag ctattccaga
     6901 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc aaagatggat
     6961 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     7021 gcctggggga atattcctct gatgagaaag gcatatttaa aaaaatgcaa ggagtttcat
     7081 cctgataaag gaggagatga agaaaaaatg aagaaaatga atactctgta caagaaaatg
     7141 gaagatggag taaaatatgc tcatcaacct gactttggag gcttctggga tgcaactgag
     7201 gtatttgctt cttccttaaa tcctggtgtt gatgcaatgt actgcaaaca atggcctgag
     7261 tgtgcaaaga aaatgtctgc taactgcata tgcttgctgt gcttactgag gatgaagcat
     7321 gaaaatagaa aattatacag gaaagatcca cttgtgtggg ttgattgcta ctgcttcgat
     7381 tgctttagaa tgtggtttgg acttgatctt tgtgaaggaa ccttacttct gtggtgtgac
     7441 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     7501 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     7561 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     7621 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     7681 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     7741 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     7801 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     7861 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     7921 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     7981 taaggaatat ttgatgtata gtgccttgac tagagatcca ttttctgtta ttgaggaaag
     8041 tttgccaggt gggttaaagg agcatgattt taatccagaa gaagcagagg aaactaaaca
     8101 agtgtcctgg aagcttgtaa cagagtatgc aatggaaaca aaatgtgatg atgtgttgtt
     8161 attgcttggg atgtacttgg aatttcagta cagttttgaa atgtgtttaa aatgtattaa
     8221 aaaagaacag cccagccact ataagtacca tgaaaagcat tatgcaaatg ctgctatatt
     8281 tgctgacagc aaaaaccaaa aaaccatatg ccaacaggct gttgatactg ttttagctaa
     8341 aaagcgggtt gatagcctac aattaactag agaacaaatg ttaacaaaca gatttaatga
     8401 tcttttggat aggatggata taatgtttgg ttctacaggc tctgctgaca tagaagaatg
     8461 gatggctgga gttgcttggc tacactgttt gttgcccaaa atggattcag tggtgtatga
     8521 ctttttaaaa tgcatggtgt acaacattcc taaaaaaaga tactggctgt ttaaaggacc
     8581 aattgatagt ggtaaaacta cattagcagc tgctttgctt gaattatgtg gggggaaagc
     8641 tttaaatgtt aatttgccct tggacaggct gaactttgag ctaggagtag ctattgacca
     8701 gtttttagta gtttttgagg atgtaaaggg cactggaggg gagtccagag atttgccttc
     8761 aggtcaggga attaataacc tggacaattt aagggattat ttggatggca gtgttaaggt
     8821 aaacttagaa aagaaacacc taaataaaag aactcaaata tttccccctg gaatagtcac
     8881 catgaatgag tacagtgtgc ctaaaacact gcaggccaga tttgtaaaac aaatagattt
     8941 taggcccaaa gattatttaa agcattgcct ggaacgcagt gagtttttgt tagaaaagag
     9001 aataattcaa agtggcattg ctttgcttct tatgttaatt tggtacagac ctgtggctga
     9061 gtttgctcaa agtattcaga gcagaattgt ggagtggaaa gagagattgg acaaagagtt
     9121 tagtttgtca gtgtatcaaa aaatgaagtt taatgtggct atgggaattg gagttttaga
     9181 ttggctaaga aacagtgatg atgatgatga agacagccag gaaaatgctg ataaaaatga
     9241 agatggtggg gagaagaaca tggaagactc agggcatgaa acaggcattg attcacagtc
     9301 ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata
     9361 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcc
//
