LOCUS       sequence-419623-        9032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human ARID1A and AAVS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: 
            pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(AAVS1)-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..9032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(32..460)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(142..163)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     354..373
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     587..609
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     601..618
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     602..618
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     628..647
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        628..646
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        962..1160
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1115..1135
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1178..1358
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1405..1530
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2029..2262
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2447..2491
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2789..2906
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3363..3611
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3363..3383
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3534..3553
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3740..4239
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3775..3798)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4172..4191
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4279..4875
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4279..4298)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4775..4795
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4894..4947
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4948..5649
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(5709..5729)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6127..6138)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6590..6607)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6596..6820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6846..6866)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6846..6864)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6885..6901)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6885..6901)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6898..6920)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6909..6925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6933..6939,6940..6957,6958..6963))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6978..6999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7116..7133)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7287..7875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7367..7386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8046..8837,8838..8906))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8669..8688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8907..9011)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
       61 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
      121 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
      181 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
      241 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
      301 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
      361 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
      421 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
      481 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
      541 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
      601 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgactagt
      661 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      721 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      781 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      841 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      901 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      961 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     1021 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     1081 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     1141 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     1201 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1261 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1321 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1381 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1441 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1501 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1561 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1621 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1681 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1741 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1801 gacaccaagg aagctttaga caagatagag gaacagcaaa acaaaagtaa gaccaccgca
     1861 cagcaagcgg ccggccgcgc tgatcttcag acctggagga ggagatatga gggacaattg
     1921 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     1981 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     2041 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     2101 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     2161 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     2221 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     2281 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     2341 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     2401 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     2461 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     2521 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     2581 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     2641 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     2701 gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct
     2761 gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     2821 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     2881 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
     2941 ggttagtacc gggccctacg cgtctgcagt atttagcatg ccccacccat ctgcaaggca
     3001 ttctggatag tgtcaaaaca gccggaaatc aagtccgttt atctcaaact ttagcatttt
     3061 gggaataaat gatatttgct atgctggtta aattagattt tagttaaatt tcctgctgaa
     3121 gctctagtac gataagcaac ttgacctaag tgtaaagttg agacttcctt caggtttata
     3181 tagcttgtgc gccgcttggg tacctcgagg gcaaactgcc cagtgtgttt aagagctatg
     3241 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca
     3301 ccgagtcggt gcttttttta gccgaactgt ttcacactca cgcgtccaag gtcgggcagg
     3361 aagagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
     3421 agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
     3481 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
     3541 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     3601 gacgaaacac cggtccctag tggccccact ggtttaagag ctatgctgga aacagcatag
     3661 caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     3721 ttttggatcc aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     3781 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     3841 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     3901 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     3961 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     4021 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     4081 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     4141 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     4201 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca tctagatctc
     4261 gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     4321 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     4381 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     4441 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     4501 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     4561 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     4621 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     4681 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     4741 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     4801 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     4861 cgcaagcccg gtgccggcgg cgggtccgga ggagagggca gaggaagtct cctaacatgc
     4921 ggtgacgtgg aggagaatcc tggcccaatg agcgagctga ttaaggagaa catgcacatg
     4981 aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa
     5041 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
     5101 cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac
     5161 cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag
     5221 agagtcacca catacgagga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
     5281 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct
     5341 gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac
     5401 ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc
     5461 gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc
     5521 gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc
     5581 gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag
     5641 cttaattgag cggccgctca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5701 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5761 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5821 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5881 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5941 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6001 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6061 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6121 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6181 cctcttccgc gtctacgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6241 ccgcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6301 aaagaaaagg ggggactgga agggctaatt cactcccaaa gaagtcaaga tctgcttttt
     6361 gcctgtactg ggtctctctg gttagaccag agtctctctg gttagaccag atctgagcct
     6421 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6481 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6541 ccttttagtc agtgtggaaa atctctagca gtttaaaccc gctgatcagc ctcgactgtg
     6601 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6661 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6721 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6781 gtcaatagca ggcatgctgg ggatgcggtg ggctctatgg gcggccgtta attaagcttt
     6841 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     6901 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgggag cataaagtgt
     6961 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7021 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7081 agaggcggtt tgcgtattgg gcgctgttcc gcttcctcgc tcactgactc gctgcgctcg
     7141 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7201 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7261 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7321 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7381 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7441 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7501 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7561 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7621 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7681 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7741 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7801 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7861 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7921 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7981 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8041 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8101 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8161 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8221 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8281 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8341 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8401 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8461 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8521 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8581 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8641 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8701 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8761 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8821 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8881 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8941 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9001 ggggttccgc gcacatttcc ccgaaaagtg cc
//
