LOCUS       sequence-419631-        9032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human RUNX1 and RUNX3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: 
            pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(13))-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..9032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(14..469)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(151..172)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     363..382
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     596..618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     610..627
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     611..627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     637..656
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        637..655
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        971..1169
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1124..1144
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1187..1367
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1414..1539
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2038..2271
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2456..2500
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2798..2915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3372..3620
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3372..3392
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3543..3562
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3749..4248
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3784..3807)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4181..4200
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4288..4884
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4288..4307)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4784..4804
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4903..4956
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4957..5658
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(5718..5738)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6136..6147)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6599..6616)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6605..6829
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6855..6875)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6855..6873)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6894..6910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6894..6910)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6907..6929)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6918..6934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6942..6948,6949..6966,6967..6972))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6987..7008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7125..7142)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7296..7884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7376..7395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8055..8846,8847..8915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8678..8697
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8916..9020)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
       61 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
      121 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
      181 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
      241 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
      301 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
      361 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
      421 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
      481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      601 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
      661 ttgactagta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      721 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      781 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      841 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      901 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      961 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1021 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1081 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1141 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     1201 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1261 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1321 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1381 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1441 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1501 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1561 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1621 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1681 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1741 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1801 gagataaaag acaccaagga agctttagac aagatagagg aacagcaaaa caaaagtaag
     1861 accaccgcac agcaagcggc cggccgcgct gatcttcaga cctggaggag gagatatgag
     1921 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1981 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     2041 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     2101 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     2161 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     2221 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     2281 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     2341 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2401 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2461 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2521 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2581 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2641 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2701 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
     2761 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     2821 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2881 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     2941 atccagtttg gttagtaccg ggccctacgc gtctgcagta tttagcatgc cccacccatc
     3001 tgcaaggcat tctggatagt gtcaaaacag ccggaaatca agtccgttta tctcaaactt
     3061 tagcattttg ggaataaatg atatttgcta tgctggttaa attagatttt agttaaattt
     3121 cctgctgaag ctctagtacg ataagcaact tgacctaagt gtaaagttga gacttccttc
     3181 aggtttatat agcttgtgcg ccgcttgggt acctcgactt cgaccgacaa acctggttta
     3241 agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa
     3301 aaagtggcac cgagtcggtg ctttttttag ccgaactgtt tcacactcac gcgtccaagg
     3361 tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     3421 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
     3481 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     3541 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3601 tgtggaaagg acgaaacacc gacgtgctgg cggaccacgc gtttaagagc tatgctggaa
     3661 acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     3721 cggtgctttt tttggatcca attctaccgg gtaggggagg cgcttttccc aaggcagtct
     3781 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     3841 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     3901 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     3961 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     4021 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     4081 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     4141 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     4201 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcatccat
     4261 ctagatctcg agcagctgaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     4321 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     4381 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     4441 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     4501 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     4561 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     4621 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     4681 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     4741 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     4801 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     4861 tgcatgaccc gcaagcccgg tgccggcggc gggtccggag gagagggcag aggaagtctc
     4921 ctaacatgcg gtgacgtgga ggagaatcct ggcccaatga gcgagctgat taaggagaac
     4981 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     5041 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     5101 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     5161 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     5221 acatgggaga gagtcaccac atacgaggac gggggcgtgc tgaccgctac ccaggacacc
     5281 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     5341 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
     5401 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     5461 catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     5521 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
     5581 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     5641 gggcacaagc ttaattgagc ggccgctcaa cctctggatt acaaaatttg tgaaagattg
     5701 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5761 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5821 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5881 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5941 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6001 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6061 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6121 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6181 gctctgcggc ctcttccgcg tctacgcctt cgccctcaga cgagtcggat ctccctttgg
     6241 gccgcctccc cgcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6301 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaaag aagtcaagat
     6361 ctgctttttg cctgtactgg gtctctctgg ttagaccaga gtctctctgg ttagaccaga
     6421 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6481 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6541 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tttaaacccg ctgatcagcc
     6601 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6661 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6721 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6781 gattgggaag tcaatagcag gcatgctggg gatgcggtgg gctctatggg cggccgttaa
     6841 ttaagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     6901 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc
     6961 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7021 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7081 cgcgcgggga gaggcggttt gcgtattggg cgctgttccg cttcctcgct cactgactcg
     7141 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7201 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7261 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7321 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7381 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7441 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7501 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7561 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7621 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7681 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7741 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7801 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7861 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7921 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7981 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8041 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8101 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8161 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8221 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8281 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8341 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8401 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8461 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8521 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8581 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8641 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8701 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8761 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8821 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8881 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8941 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9001 aaacaaatag gggttccgcg cacatttccc cg
//
