LOCUS       sequence-419679-       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pPBpuro-miRFP703-eDHFR(69K6)-cRaf
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(31..459)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(141..162)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     353..372
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     586..608
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     600..617
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     601..617
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     627..646
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        627..645
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   675..709
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1012..1391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1722..2738
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2662..2684
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2746..2765
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2829..2838
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2835..3779
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    6434..6983
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6578..6595)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6805..6824
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6986..7585
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6986..7005)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7482..7502
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7612..7629)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7618..7842
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7889..7905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7913..7929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7937..7943,7944..7961,7962..7967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7982..8003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8237..8299
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8340..8360)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8340..8358)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8379..8395)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8403..8419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8427..8433,8434..8451,8452..8457))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8472..8493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8610..8627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8781..9369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8861..8880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9540..10331,10332..10400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10163..10182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10401..10505)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
       61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
      121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
      181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
      241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
      301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
      361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
      421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
      481 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
      541 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
      601 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggggcgcg
      661 ccattctaga ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa
      721 tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc
      781 gccgcttgga gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac
      841 tataacgacc gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt
      901 aactcatacg ataattatat tgttatttca tgttctactt acgtgataac ttattatata
      961 tatattttct tgttatagat atcaactaga atgctagcat gggcccatct cgacattgat
     1021 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     1081 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     1141 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     1201 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     1261 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     1321 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     1381 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     1441 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     1501 gggggggggg gggggcgggc gcaggggggg ggggcggggg gggggggggg gggggggggg
     1561 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
     1621 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     1681 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
     1741 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     1801 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     1861 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     1921 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     1981 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     2041 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     2101 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     2161 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     2221 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     2281 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     2341 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     2401 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     2461 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     2521 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     2581 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     2641 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     2701 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     2761 ttgtgctgtc tcatcatttt ggcaaagaat tgggatcctc acgtcaattg tagctagcgc
     2821 taccggtcgc caccatggta gcaggtcatg cctctggcag ccccgcattc gggaccgcct
     2881 ctcattcgaa ttgcgaacat gaagagatcc acctcgccgg ctcgatccag ccgcatggcg
     2941 cgcttctggt cgtcagcgaa catgatcatc gcgtcatcca ggccagcgcc aacgccgcgg
     3001 aatttctgaa tctcggaagc gtactcggcg ttccgctcgc cgagatcgac ggcgatctgt
     3061 tgatcaagat cctgccgcat ctcgatccca ccgccgaagg catgccggtc gcggtgcgct
     3121 gccggatcgg caatccctct acggagtact gcggtctgat gcatcggcct ccggaaggcg
     3181 ggctgatcat cgaactcgaa cgtgccggcc cgtcgatcga tctgtcaggc acgctggcgc
     3241 cggcgctgga gcggatccgc acggcgggtt cactgcgcgc gctgtgcgat gacaccgtgc
     3301 tgctgtttca gcagtgcacc ggctacgacc gggtgatggt gtatcgtttc gatgagcaag
     3361 gccacggcct ggtattctcc gagtgccatg tgcctgggct cgaatcctat ttcggcaacc
     3421 gctatccgtc gtcgctggtc ccgcagatgg cgcggcagct gtacgtgcgg cagcgcgtcc
     3481 gcgtgctggt cgacgtcacc tatcagccgg tgccgctgga gccgcggctg tcgccgctga
     3541 ccgggcgcga tctcgacatg tcgggctgct tcctgcgctc gatgtcgccg atccatctgc
     3601 agttcctgaa ggacatgggc gtgcgcgcca ccctggcggt gtcgctggtg gtcggcggca
     3661 agctgtgggg cctggttgtc tgtcaccatt atctgccgcg cttcatccgt ttcgagctgc
     3721 gggcgatctg caaacggctc gccgaaagga tcgcgacgcg gatcaccgcg cttgagagcc
     3781 tgtacaagtc tgggtccgca gccagtagcg ccggaagtat gatcagtctg attgcggcgt
     3841 tagcggtaga tcgcgttatc ggcatggaaa acgccatgcc gtggaacctg cctgccgatc
     3901 tcgcctggtt taaacgcaac accttaaata aacccgtgat tatgggccgc catacctggg
     3961 aatcaatcgg tcgtccgttg ccaggacgca aaaatattat cctcagcagt caaccgggta
     4021 cggacaaaaa aaagaaaaag aaagatcgcg taacgtgggt gaagtcggtg gatgaagcca
     4081 tcgcggcgtg tggtgacgta ccagaaatca tggtgattgg cggcggtcgc gtttatgaac
     4141 agttcttgcc aaaagcgcaa aaactgtatc tgacgcatat cgacgcagaa gtggaaggcg
     4201 acacccattt cccggattac gagccggatg actgggaatc ggtattcagc gaattccacg
     4261 atgctgatgc gcagaactct cacagctatt gctttgagat tctggagcgg cggagtgctg
     4321 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggtcctcgaa
     4381 gcctcgagat ggagcacata cagggagctt ggaagacgat cagcaatggt tttggattca
     4441 aagatgccgt gtttgatggc tccagctgca tctctcctac aatagttcag cagtttggct
     4501 atcagcgccg ggcatcagat gatggcaaac tcacagatcc ttctaagaca agcaacacta
     4561 tccgtgtttt cttgccgaac aagcaaagaa cagtggtcaa tgtgcgaaat ggaatgagct
     4621 tgcatgactg ccttatgaaa gcactcaagg tgaggggcct gcaaccagag tgctgtgcag
     4681 tgttcagact tctccacgaa cacaaaggta aaaaagcacg cttagattgg aatactgatg
     4741 ctgcgtcttt gattggagaa gaacttcaag tagatttcct ggatcatgtt cccctcacaa
     4801 cacacaactt tgctcggaag acgttcctga agcttgcctt ctgtgacatc tgtcagaaat
     4861 tcctgctcaa tggatttcga tgtcagactt gtggctacaa atttcatgag cactgtagca
     4921 ccaaagtacc tactatgtgt gtggactgga gtaacatcag acaactctta ttgtttccaa
     4981 attccactat tggtgatagt ggagtcccag cactaccttc tttgactatg cgtcgtatgc
     5041 gagagtctgt ttccaggatg cctgttagtt ctcagcacag atattctaca cctcacgcct
     5101 tcacctttaa cacctccagt ccctcatctg aaggttccct ctcccagagg cagaggtcga
     5161 catccacacc taatgtccac atggtcagca ccaccctgcc tgtggacagc aggatgattg
     5221 aggatgcaat tcgaagtcac agcgaatcag cctcaccttc agccctgtcc agtagcccca
     5281 acaatctgag cccaacaggc tggtcacagc cgaaaacccc cgtgccagca caaagagagc
     5341 gggcaccagt atctgggacc caggagaaaa acaaaattag gcctcgtgga cagagagatt
     5401 caagctatta ttgggaaata gaagccagtg aagtgatgct gtccactcgg attgggtcag
     5461 gctcttttgg aactgtttat aagggtaaat ggcacggaga tgttgcagta aagatcctaa
     5521 aggttgtcga cccaacccca gagcaattcc aggccttcag gaatgaggtg gctgttctgc
     5581 gcaaaacacg gcatgtgaac attctgcttt tcatggggta catgacaaag gacaacctgg
     5641 caattgtgac ccagtggtgc gagggcagca gcctctacaa acacctgcat gtccaggaga
     5701 ccaagtttca gatgttccag ctaattgaca ttgcccggca gacggctcag ggaatggact
     5761 atttgcatgc aaagaacatc atccatagag acatgaaatc caacaatata tttctccatg
     5821 aaggcttaac agtgaaaatt ggagattttg gtttggcaac agtaaagtca cgctggagtg
     5881 gttctcagca ggttgaacaa cctactggct ctgtcctctg gatggcccca gaggtgatcc
     5941 gaatgcagga taacaaccca ttcagtttcc agtcggatgt ctactcctat ggcatcgtat
     6001 tgtatgaact gatgacgggg gagcttcctt attctcacat caacaaccga gatcagatca
     6061 tcttcatggt gggccgagga tatgcctccc cagatcttag taagctatat aagaactgcc
     6121 ccaaagcaat gaagaggctg gtagctgact gtgtgaagaa agtaaaggaa gagaggcctc
     6181 tttttcccca gatcctgtct tccattgagc tgctccaaca ctctctaccg aagatcaacc
     6241 ggagcgcttc cgagccatcc ttgcatcggg cagcccacac tgaggatatc aatgcttgca
     6301 cgctgaccac gtccccgagg ctgcctgtct tctgcggccg ctagtcgacg gtaccgcggg
     6361 cccgggataa gtcaactaac ttaagctagc aacggtttcc ctctagcggg atcaattccg
     6421 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     6481 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     6541 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     6601 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     6661 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     6721 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     6781 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     6841 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     6901 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     6961 ttcctttgaa aaacacgata ataccatgac cgagtacaag cccacggtgc gcctcgccac
     7021 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     7081 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     7141 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     7201 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     7261 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     7321 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     7381 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     7441 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     7501 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     7561 catgacccgc aagcccggtg cctgatcgat gatctagagc tcgctgatca gcctcgactg
     7621 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     7681 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     7741 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     7801 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     7861 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7921 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7981 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8041 acctgtcgtg ccagcggatc ttaaaagttt tgttacttta tagaagaaat tttgagtttt
     8101 tgtttttttt taataaataa ataaacataa ataaattgtt tgttgaattt attattagta
     8161 tgtaagtgta aatataataa aacttaatat ctattcaaat taataaataa acctcgatat
     8221 acagaccgat aaaacacatg cgtcaatttt acgcatgatt atctttaacg tacgtcacaa
     8281 tatgattatc tttctagggt taatctagta tacgcgttta attaatccag cttttgttcc
     8341 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     8401 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8461 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8521 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8581 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8641 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8701 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8761 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8821 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8881 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8941 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9001 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9061 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9121 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9181 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9241 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9301 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9361 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9421 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9481 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9541 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9601 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9661 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9721 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9781 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9841 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9901 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9961 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10021 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10081 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10141 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10201 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10261 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10321 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10381 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10441 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10501 ccgcgcacat ttccccgaaa agtgcca
//
