LOCUS       sequence-419906-        5193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hcoRBFOX1N-PUFc-RBFOX1C(190-397).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Liu Z, Jillette N, Robson P, Cheng AW
  TITLE     Simultaneous multifunctional transcriptome engineering by CRISPR RNA
            scaffold.
  JOURNAL   Nucleic Acids Res. 2023 Aug 11;51(14):e77. doi: 10.1093/nar/gkad547.
  PUBMED    37395412
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2023-08-11"; volume: "51(14):e77. doi"; pages: "
            10.1093/nar/gkad547"
COMMENT     Sequence Label: pmax-hcoRBFOX1N-PUFc-RBFOX1C
FEATURES             Location/Qualifiers
     source          1..5193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          813..945
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    960..984
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1395..1415
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2472..2492
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2496..2516
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3222..3246)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3299..3340
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3366..3385)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    3395..3476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3420..3439
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3674..4262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3754..3773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4343..5137)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4343..5137)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4454..4473)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5064..5083
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
      901 cacctattgg tcttactgac atccactttg cctttctctc cacagggaaa aaacaattga
      961 caagtttgta caaaaaagca ggctccgaat tcaccggtgc cgccaccatg aattgcgagc
     1021 gggaacagct caggggcaat caagaagcgg cagctgcacc tgacacaatg gcacagccgt
     1081 atgcgagtgc tcaatttgcg ccaccccaga atggcatacc cgcagaatac acagcgccgc
     1141 atcctcaccc cgcccctgaa tacactgggc aaactaccgt tcctgaacac acgcttaacc
     1201 tctatcctcc tgctcagact cactcagaac agagtccagc agatacaagt gcgcagacag
     1261 tctctggcac tgcgacccag accgatgacg cagctcctac tgatggccag ccacaaactc
     1321 aaccctctga aaacactgaa aataaaagcc aacccaaggg cggtggaggc tccgggcgcg
     1381 ccgggatcct cccccccaag aaaaagagga aggtatctag aggccgcagc cgccttttgg
     1441 aagattttcg aaacaaccgg taccccaatt tacaactgcg ggagattgct ggacatataa
     1501 tggaattttc ccaagaccag catgggtcca gattcattca gctgaaactg gagcgtgcca
     1561 caccagctga gcgccagctt gtcttcaatg aaatcctcca ggctgcctac caactcatgg
     1621 tggatgtgtt tggtaattac gtcattcaga agttctttga atttggcagt cttgaacaga
     1681 agctggcttt ggcagaacgg attcgaggcc acgtcctgtc attggcacta cagatgtatg
     1741 gcagccgtgt tatcgagaaa gctcttgagt ttattccttc agaccagcag aatgagatgg
     1801 ttcgggaact agatggccat gtcttgaagt gtgtgaaaga tcagaatggc aatcacgtgg
     1861 ttcagaaatg cattgaatgt gtacagcccc agtctttgca atttatcatc gacgcgttta
     1921 agggacaggt atttgcctta tccacacatc cttatggctg ccgagtgatt cagagaatcc
     1981 tggagcactg tctccctgac cagacactcc ctattttaga ggagcttcac cagcacacag
     2041 agcagctggt acaggatcaa tatggaagtt atgtaatcga acatgtactg gagcacggtc
     2101 gtcctgagga taaaagcaaa attgtagcag aaatccgagg caatgtactt gtattgagtc
     2161 agcacaaatt tgcaaacaat gttgtgcaga agtgtgttac tcacgcctca cgtacggagc
     2221 gcgctgtgct catcgacgag gtgtgcacca tgaacgacgg tccccacagt gccttataca
     2281 ccatgatgaa ggaccagtat gccaactacg tggtccagaa gatgattgac gtggcggagc
     2341 caggccagcg gaagatcgtc atgcataaga tccggcccca catcgcaact cttcgtaagt
     2401 acacctatgg caagcacatt ctggccaagc tggagaagta ctacatgaag aacggtgttg
     2461 acttagggga cccaaagaag aagcgcaaag tggatcctaa aaagaaaaga aaggtaggcg
     2521 gccgcggggg aggcggttcc ggtggcggcg gaagcggagg tggaggatca gggccggcca
     2581 atgccacggc tagggtcatg acaaacaaga agacggttaa tccgtatacc aacgggtgga
     2641 aacttaatcc cgttgtcggc gctgtctatt ctcccgagtt ctatgctggt acagtcttgc
     2701 tgtgccaagc caatcaagag gggtcttcaa tgtacagtgc tccgagcagc ttggtttaca
     2761 caagcgcgat gccaggattt ccttaccctg cggcaacggc cgcggctgcg tatcggggtg
     2821 cccaccttcg cgggcggggc cgaacagttt acaacacgtt cagggccgct gctccacctc
     2881 ccccgattcc cgcgtacgga ggtgttgtct accaagacgg gttttatgga gccgacatct
     2941 acggtggtta cgccgcatat cggtatgcac aaccaacccc cgcgaccgcg gcagcgtaca
     3001 gcgactccta cggacgcgtg tacgctgctg atccttacca ccacgccctg gcccccgccc
     3061 caacgtatgg tgtcggtgct atgaatgcgt tcgcgcccct gaccgatgca aaaaccaggt
     3121 cacatgccga cgacgttgga ttggtattga gctctttgca agcatccatt taccgggggg
     3181 gatacaaccg cttcgcgcct tattgattaa ttaagaattc gacccagctt tcttgtacaa
     3241 agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     3301 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt agcaattgtt
     3361 ttttcgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     3421 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     3481 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     3541 gcaagtaaaa cctctacaaa tgtggtactt aagaggggga gaccaaaggg cgagacgtta
     3601 aggcctcacg tgacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3661 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3721 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3781 gctccctcgt gcgctctcct gttccgaccc tgccgcttac gggatacctg tccgcctttc
     3841 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3901 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3961 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4021 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4081 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4141 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4201 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4261 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4321 aagggatttt ggtcatgccg tctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     4381 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     4441 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     4501 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     4561 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc
     4621 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     4681 caagaccggc ttccatccga gtacgtgctc tctcgatgcg atgtttcgct tggtggtcga
     4741 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     4801 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     4861 gcagccagtc ccttcccgct tcagtgacaa cgtcgagtac agctgcgcaa ggaacgcccg
     4921 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     4981 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     5041 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     5101 ccggagaacc tgcgtgcaat ccatcttgtt caatcataat attattgaag catttatcag
     5161 ggttcgtctc gtcccggtct cctcccatgc atg
//
