LOCUS       sequence-419913-        7214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Construct for stable expression of constitutively active human PTK6 
            in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Zheng Y, Peng M, Wang Z, Asara JM, Tyner AL
  TITLE     Protein tyrosine kinase 6 directly phosphorylates AKT and promotes 
            AKT activation in response to epidermal growth factor.
  JOURNAL   Mol Cell Biol. 2010 Sep;30(17):4280-92. doi: 10.1128/MCB.00024-10. 
            Epub 2010 Jul 6.
  PUBMED    20606012
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00024-10"; journalName: "Mol Cell Biol"; date: 
            "2010-09"; volume: "30"; issue: "17"; pages: "4280-92"
COMMENT     Sequence Label: pBabe-PTK6-YF
FEATURES             Location/Qualifiers
     source          1..7214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             890..1359
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(1580..2179)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1663..1683)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2160..2179
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2189..2385)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(2197..2407)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2203..2338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2431..2451
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(4608..4637)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(4709..5125)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(4725..4741)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4757..4779)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    5326..5525
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             5590..6059
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        6090..6194
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6195..6263,6264..7055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6413..6432)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg gccatttaat
      841 gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt gaaagacccc
      901 cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga gaccacaagt
      961 cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact cagtcaatcg
     1021 gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg ggagcagaag
     1081 cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca cagggtcatt
     1141 tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc tgagggctgg
     1201 accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag
     1261 atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag ccggggcagg
     1321 aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct gttcctgacc
     1381 ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa aatggcgtta
     1441 cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt ttctggagac
     1501 taaataaaat cttttatttt atcgatgcat ggggtcgtgc gctcctttcg gtcgggcgct
     1561 gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc
     1621 cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt
     1681 tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc gcctccactc
     1741 cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag acgccgacgg
     1801 tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct tccatctgtt
     1861 gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc tcggcgaaca
     1921 ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg
     1981 cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg
     2041 tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg tagtcggcga
     2101 acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg aggcgcaccg
     2161 tgggcttgta ctcggtcatg gtaagctttt tgcaaaagcc taggcctcca aaaaagcctc
     2221 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     2281 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     2341 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     2401 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagggtcg
     2461 accactgtgc tggcgaatta gctttttatg acttaaccat ggatgaaaga gacaccttca
     2521 aaatcaggtg caaaggaggc agccccccca gcccggaacc accccacaga cagtagagaa
     2581 gggcccttgt cagcgcagga gtgaggggcg ctttccagca ctgacccagc gacaaaggtg
     2641 aaggtgggaa aagccccttg tggacgtgga cggtgaaact gagctggcgc ctcgggcagt
     2701 gatgcttggg ctggcagaga ccagggagag agttctcact ggtcataagg ccaagctctc
     2761 aagacacaag acaaacagga aagaaacacc tgcccctggg aggaaagaac ccttgagcac
     2821 caaaaccaaa tttacatgag cgtcccgagc cacgcgaatg actttctccc accaagctga
     2881 actgggaaga gcaggaacaa ggcgtgtttg cctcccaccc tcagccgggc actgcagaca
     2941 gacagccaga gagcgggctg tggtcagagt cccctctgac ctctgctggc ttcgtgtcag
     3001 tctcccggat ctcatctgca ggctccagag caggctgcgt gtcagcagct gagacccaag
     3061 gcacacacga tggagtaagg agaggagcac acgcgtgtat tggacgcaga cactccacat
     3121 ttgtgaacct ttcctgcacc catcacctca gtaaacccca gggaagcgcg tgggccttga
     3181 tcccaggtcc aggccctctg cccaggcccc tcctcagcag ggcccggcca tgcccgctcc
     3241 acagcagctc aggtcgggtt ctcgaagctg gtgaagctgg agagcctctc ccgcagggcc
     3301 ttgaagcagg gtctctgctc ggggtccctg caccagcatg tcagcatcag cttgtgcacg
     3361 ctgggcgggc actccagagg gcagggcatg cggtagccgg cgtccaccct caggaaggcc
     3421 tcatggttgg acatgcctgg gtagggcacc tgacccctgc tgaacatctc atgcaggaga
     3481 atcccaaagg accagacgtc ggatttggtg gagtaatggc ctcgggagag cgcttcaggg
     3541 gccgtccact tgtaggggat attgtggtca tgggagaggt agacgtcctc cttgataagc
     3601 ctggctaacc cgaagtcccc aactttgcag agggtgtttt ccccgacgag gatgttcctg
     3661 gcggccaggt cccggtggat gtaattctgc gactccaggt aacacatgcc ctcagccacc
     3721 tgccaggcga tgtccagcag ctccgaaacg ggcaggactt tctcatcaga gtcgcggagc
     3781 agctccagca ggctgccctt ggccatgagc tccgtgatga tgtacacggg gtcccccacg
     3841 gacaccacgg cgtacagcgc caggatgtgt ttgtgccgca gcttcttcat ggcctggatc
     3901 tccgactgca gcatctgctg gtgcaggagg ttgtctcgag aaatcacctt aatggccacc
     3961 tggacccggt ctttccagag cccctcgaag acctccccaa agtagccgga ccccagcttc
     4021 ctgcagagcg tgaactcctc cctcggcctc tcccagtcat cccaatgggg caggggctca
     4081 ggctcgtgct tccggcaggg cgcggccagc cgcaggccgt gggacaggct ctgggccctg
     4141 tggtagttca caagctcggg caggctgagg aaggacaccg cctcgttcag gtgcagccgg
     4201 cccccggcac gccgccagat cttgtagtgc cgcacagcct gcgtgtcccg caccgacagg
     4261 acgtagtcgg cactcggctt ctcgctgacc ctgatcagga aggcgcccgt ggcgttgccc
     4321 tcggcctgca gccgacgcac agcttccgag cgggagatgc agccaaagaa ccacggttcc
     4381 gactccaccg tctccctctc ggccaggtag ttgtggggca catagccctg ggccacggcc
     4441 ccacccgcct cgtccagcag cgtggcccac caccactgct cctccttcct ggccacgtgg
     4501 aagacgtccc ccgcgcggaa gctcagctcc tcgtccgtcc gggacttgaa gtcccagagg
     4561 cccacatact tggggcccag gtgagcctgg tcccaattcc cgttgttcag gtcctcttcg
     4621 ctaatcagct tttgttccat agacgccatg gtggatccga gctcggtacc aagctaattc
     4681 ctacgtacca ccacactggg atccggccgg cgcctagaga aggagtgagg gctggataaa
     4741 gggaggatcg aggcggggtc gaacgaggag gttcaagggg gagagacggg gcggatggag
     4801 gaagaggagg cggaggctta gggtgtacaa agggcttgac ccagggaggg gggtcaaaag
     4861 ccaaggcttc ccaggtcacg atgtagggga cctggtctgg gtgtccatgc gggccaggtg
     4921 aaaagacctt gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg tctcgcggcc
     4981 atccgacgtt aaaggttggc cattctgcag agcagaaggt aacccaacgt ctcttcttga
     5041 catctaccga ctggttgtga gcgatccgct cgacatcttt ccagtgacct aaggtcaaac
     5101 ttaagggagt ggtaacagtc tggccctaat tttcagacaa atacagaaac acagtcagac
     5161 agagacaaca cagaacgatg ctgcagcgct gcagcagaca agacgcgcgg cttcggttcc
     5221 aaaccgaaag caaaaattca gacggaggcg ggaactgttt taggttctcg tctcctacca
     5281 gaaccacata tcctgacggg gtcggattcc acatcgactc ccttcctcag gtcgggccac
     5341 aaaaacggcc cccaaagtcc ctgggacgtc tcccagggtt gcggccgggt gttcagaact
     5401 cgtcagttcc accacgggtc cgccagatac agagctagtt agctaactag taccgacgca
     5461 ggcgcataaa atcagtcata gacactagac aatcggacag acacagataa gttgctggcc
     5521 agcttgcctc ccggtggtgg gtcggtggtc cctgggcagg ggtctcccga tcccggacga
     5581 gcccccaaat gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa
     5641 ggaacagcga gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac
     5701 ccgggcgact cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta
     5761 ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc
     5821 aaataaggca cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc
     5881 tagaaactgc tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg
     5941 gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct
     6001 tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagg
     6061 ctgcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6121 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6181 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6241 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6301 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6361 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6421 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6481 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6541 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6601 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6661 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6721 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6781 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6841 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6901 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6961 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7021 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7081 tactttagat tgatttgcgg ccgcaaaagg atctaggtga agatcctttt tgataatctc
     7141 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7201 atcaaaggat cttc
//
