LOCUS       sequence-420184-       10121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector to express MLV Gag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pLV_EF1a_Gag
FEATURES             Location/Qualifiers
     source          1..10121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1720..1837
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1976..3154
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2207..3145
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3102..3122
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3161..3170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3170..3586
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3516..3538
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3554..3570
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4766..4822
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      5072..5081
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5534..5590
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5591..5962
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="AKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDV
                     TLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGRE
                     FALRDPAGNCVHFVAEEQD"
     misc_feature    5978..6566
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6031..6051)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6449..6460)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6638..6871
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6949..7070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6986..7005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7040..7059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7110..7245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7265..7284)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7266..7284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7294..7311)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7294..7310)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7303..7325)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7452..7907
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7539..7558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7749..7770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7933..8037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8038..8106,8107..8898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPTGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8256..8275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9069..9657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9558..9577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9811..9828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9945..9966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9981..9986,9987..10004,10005..10011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10019..10035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10024..10046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10043..10059
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10043..10059
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10078..10098
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10080..10098
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     1741 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1801 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     1861 agatccactt tggcgccggc tcgagggggc ccgggtgcaa agatggataa agttttaaac
     1921 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
     1981 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     2041 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     2101 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     2161 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     2221 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     2281 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     2341 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     2401 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     2461 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     2521 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     2581 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     2641 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     2701 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     2761 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     2821 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     2881 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     2941 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     3001 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     3061 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     3121 tcaaagtttt tttcttccat ttcaggtgtc gtgacgtacg gccaccatgg gccagactgt
     3181 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa
     3241 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac
     3301 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa
     3361 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac
     3421 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc
     3481 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc
     3541 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccaaac ctaaacctca
     3601 agttctttct gacagtgggg ggccgctcat cgacctactt acagaagacc ccccgcctta
     3661 tagggaccca agaccacccc cttccgacag ggacggaaat ggtggagaag cgacccctgc
     3721 gggagaggca ccggacccct ccccaatggc atctcgccta cgtgggagac gggagccccc
     3781 tgtggccgac tccactacct cgcaggcatt ccccctccgc gcaggaggaa acggacagct
     3841 tcaatactgg ccgttctcct cttctgacct ttacaactgg aaaaataata acccttcttt
     3901 ttctgaagat ccaggtaaac tgacagctct gatcgagtct gtcctcatca cccatcagcc
     3961 cacctgggac gactgtcagc agctgttggg gactctgctg accggagaag aaaaacaacg
     4021 ggtgctctta gaggctagaa aggcggtgcg gggcgatgat gggcgcccca ctcaactgcc
     4081 caatgaagtc gatgccgctt ttcccctcga gcgcccagac tgggattaca ccacccaggc
     4141 aggtaggaac cacctagtcc actatcgcca gttgctccta gcgggtctcc aaaacgcggg
     4201 cagaagcccc accaatttgg ccaaggtaaa aggaataaca caagggccca atgagtctcc
     4261 ctcggccttc ctagagagac ttaaggaagc ctatcgcagg tacactcctt atgaccctga
     4321 ggacccaggg caagaaacta atgtgtctat gtctttcatt tggcagtctg ccccagacat
     4381 tgggagaaag ttagagaggt tagaagattt aaaaaacaag acgcttggag atttggttag
     4441 agaggcagaa aagatcttta ataaacgaga aaccccggaa gaaagagagg aacgtatcag
     4501 gagagaaaca gaggaaaaag aagaacgccg taggacagag gatgagcaga aagagaaaga
     4561 aagagatcgt aggagacata gagagatgag caagctattg gccactgtcg ttagtggaca
     4621 gaaacaggat agacagggag gagaacgaag gaggtcccaa ctcgatcgcg accagtgtgc
     4681 ctactgcaaa gaaaaggggc actgggctaa agattgtccc aagaaaccac gaggacctcg
     4741 gggaccaaga ccgcagggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga
     4801 tgtcgaagag aatcctggac cggtcagcaa gggcgaggag gacaacgcct ctctcccagc
     4861 gacacacgag ttacacatct ttggctccat caacggtgtg gacttcgacc tcgtgggtca
     4921 gggcaccggc aatccaaacg acggttacga ggagctcaac ctcaagtcca ccaagggcga
     4981 cctccagttc tccccctgga ttctggtccc tcatatcggg tacggcttcc atcagtacct
     5041 gccctaccca gacgggatgt cgcctttcca ggccgccatg gtggacggct ccggatacca
     5101 agtccatcgc acaatgcagt tcgaagacgg tgcctccctt actgtcaact accgctacac
     5161 ctacgaggga agccacatca aaggagaggc ccaggtcaag gggactggtt tccctgccga
     5221 cggtcctgtc ctcaccaact cgctcaccgc tgcggactgg tgcaggtcga agaagactta
     5281 ccccaacgac aaaaccatca tcagtacctt caagtggagt tacaccactg gaaacggcaa
     5341 gcgctaccgg agcactgcgc ggaccaccta cacctttgcc aagccaatgg cggccaacta
     5401 tctcaagaac cagccgatgt acgtgttccg caagacggag ctcaagcact ccaagaccga
     5461 gctcaacttc aaggagtggc aaaaggcctt taccgacgtc atgggcatgg acgagctcta
     5521 caagggatcc ggcgcaacaa acttctctct gctcaaacaa gccggcgacg tcgaagagaa
     5581 tcctgggccg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     5641 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     5701 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     5761 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     5821 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     5881 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     5941 cgtggccgag gagcaggact gaacgcgtta agtcgacaat caacctctgg attacaaaat
     6001 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6061 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6121 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6181 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6241 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6301 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6361 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6421 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6481 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6541 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     6601 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6661 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6721 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6781 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6841 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt
     6901 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6961 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7021 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7081 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7141 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7201 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     7261 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     7321 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     7381 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     7441 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7501 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7561 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7621 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7681 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7741 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7801 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7861 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     7921 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7981 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8041 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8101 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8161 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8221 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8281 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8341 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8401 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8461 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8521 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8581 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8641 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8701 gcccttccga ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8761 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8821 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8881 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8941 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9001 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9061 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9121 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9181 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9241 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9301 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9361 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9421 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9481 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9541 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9601 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9661 gccagcaacg cggccttttt acggttcctg gccctttgct ggccttttgc tcacatgttc
     9721 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9781 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9841 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgacac
     9901 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     9961 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    10021 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
    10081 attaaccctc actaaaggga acaaaagctg gagctgcaag c
//
