LOCUS       sequence-42039-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00193
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(197..220)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa tgctttctta
       61 taatgccaac tttgtacaag aaagttggca acaccccgta cttgatgccg tactcctggt
      121 agtggcccac atgcggtcgg tagtggctca gattgctcag ctgacccagc tggcccaggt
      181 tcccgctaag cgccgcatgg tgatgatggt ggtggtggtg gctactgatc accgccgccg
      241 ctgctgccgc tgatgccgct gccacggatg cggaggcagg tggactggcg gattgtaggc
      301 tgccggtggg actaccggtg tccagttggc aggattggtt ctgctcgaag atcatgtcgc
      361 ggaaacccag tgccgatgtg ggactgctgg tcagcacgct ggaggatcct ccaccgccac
      421 cgccgccgcc tccgccaccg ttgcccactc cattgtggtt gagtacgctg ctgctgttgt
      481 tgttattatg tcccgggtgg gtgggtgtgc cctgctgccc attgtgagga acgggttccg
      541 atggtggcgt ttccggggta taccaacccc cgtgcgcggg accatgcgga gttgagatcg
      601 tggtgggcga gtgtccggac gattggcctc ccgctccttg gccgccgggg gtcagaggat
      661 gggccacgtg cgccacatga cgctgcagtt gctggg
//
