LOCUS       sequence-42041-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HDGF2
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..91
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             400..417
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(470..488)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      484..531
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(655..674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atataccatg catcatcatc atcatcacag
       61 cagcggcaga gaaaacttgt atttccaggg catgccacac gccttcaagc ccggggactt
      121 ggtgttcgct aagatgaagg gctaccctca ctggcctgcc aggatcgacg acatcgcgga
      181 tggcgccgtg aagcccccac ccaacaagta ccccatcttt ttctttggca cacacgaaac
      241 agccttcctg ggacccaagg acctgttccc ctacgacaaa tgtaaagaca agtacgggaa
      301 gcccaacaag aggaaaggct tcaatgaagg gctgtgggag atccagaaca acccccacgc
      361 cagctacagc tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      421 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      481 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      541 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      781 attagggtga tggttc
//
