LOCUS       sequence-420454-       11687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. MCS upstream of gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader 
            LC
  TITLE     An improved toolkit of gateway- and gibson assembly-compatible 
            vectors for protoplast transfection and agrobacterium-mediated plant
            transformation.
  JOURNAL   BMC Res Notes. 2025 Apr 8;18(1):149. doi: 
            10.1186/s13104-025-07231-1.
  PUBMED    40200324
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2025-04-8"; volume: "18(1):149. doi"; pages: " 
            10.1186/s13104-025-07231-1"
COMMENT     Sequence Label: pJRA-GW
FEATURES             Location/Qualifiers
     source          1..11687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    1367..1491
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1516..1521,1522..1539,1540..1546)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1600..2259
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1666..1685)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2601..2906
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2785..2804
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2947..3071)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3124..3831
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3856..3873)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3856..3872)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3865..3887)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4075..4099
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5400..6029
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6458..7531
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7597..7791
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8027..8049)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8135..8275
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8190..8209
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8290..8307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8461..9049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8541..8560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10528..11319
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgacgtc cctaggagat cttcgaacac gtgaggcctc gagatcacaa gtttgtacaa
     1381 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     1441 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca
     1501 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
     1561 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     1621 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     1681 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     1741 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     1801 aatgctcatc cggagttccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     1861 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     1921 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     1981 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     2041 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     2101 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     2161 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     2221 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc cggcttacta
     2281 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     2341 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga
     2401 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     2461 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     2521 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     2581 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     2641 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     2701 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     2761 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     2821 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     2881 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
     2941 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     3001 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     3061 tacaaagtgg tgcctaggtg agtctagaga gttaattaag acccgggact agtccctaga
     3121 gtcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct
     3181 gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg
     3241 ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt
     3301 caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt
     3361 gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt
     3421 tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg
     3481 attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc
     3541 gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg
     3601 tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc
     3661 aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt
     3721 tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat
     3781 cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcag
     3841 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3901 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3961 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     4021 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
     4081 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     4141 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     4201 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     4261 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     4321 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     4381 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     4441 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     4501 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     4561 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     4621 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     4681 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     4741 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     4801 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     4861 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     4921 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     4981 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     5041 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     5101 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     5161 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     5221 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     5281 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     5341 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     5401 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     5461 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     5521 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     5581 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     5641 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     5701 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     5761 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     5821 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     5881 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     5941 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     6001 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     6061 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     6121 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     6181 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     6241 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     6301 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     6361 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
     6421 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     6481 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     6541 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     6601 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     6661 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     6721 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     6781 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     6841 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     6901 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     6961 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     7021 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     7081 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     7141 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     7201 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     7261 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     7321 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     7381 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     7441 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     7501 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     7561 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     7621 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     7681 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     7741 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     7801 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     7861 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     7921 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     7981 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     8041 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     8101 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     8161 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8221 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     8281 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8341 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8401 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8461 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8521 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8581 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8641 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8701 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8761 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8821 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8881 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8941 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9001 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9061 gatcttttct acggggtcct gatgaatccc ctaatgattt tggtaaaaat cattaagtta
     9121 aggtggatac acatcttgtc atatgatcaa atggtttcgc gaaaaatcaa taatcagaca
     9181 acaagatgtg cgaactcgat attttacacg actctcttta ccaattctgc cccgaattac
     9241 acttaaaacg actcaacagc ttaacgttgg cttgccacgc attacttgac tgtaaaactc
     9301 tcactcttac cgaacttggc cgtaacctgc caaccaaagc gagaacaaaa cataacatca
     9361 aacgaatcga ccgattgtta ggtaatcgtc acctccacaa agagcgactc gctgtatacc
     9421 gttggcatgc tagctttatc tgttcgggca atacgatgcc cattgtactt gttgactggt
     9481 ctgatattcg tgagcaaaaa cgacttatgg tattgcgagc ttcagtcgca ctacacggtc
     9541 gttctgttac tctttatgag aaagcgttcc cgctttcaga gcaatgttca aagaaagctc
     9601 atgaccaatt tctagccgac cttgcgagca ttctaccgag taacaccaca ccgctcattg
     9661 tcagtgatgc tggctttaaa gtgccatggt ataaatccgt tgagaagctg ggttggtact
     9721 ggttaagtcg agtaagagga aaagtacaat atgcagacct aggagcggaa aactggaaac
     9781 ctatcagcaa cttacatgat atgtcatcta gtcactcaaa gactttaggc tataagaggc
     9841 tgactaaaag caatccaatc tcatgccaaa ttctattgta taaatctcgc tctaaaggcc
     9901 gaaaaaatca gcgctcgaca cggactcatt gtcaccaccc gtcacctaaa atctactcag
     9961 cgtcggcaaa ggagccatgg gttctagcaa ctaacttacc tgttgaaatt cgaacaccca
    10021 aacaacttgt taatatctat tcgaagcgaa tgcagattga agaaaccttc cgagacttga
    10081 aaagtcctgc ctacggacta ggcctacgcc atagccgaac gagcagctca gagcgttttg
    10141 atatcatgct gctaatcgcc ctgatgcttc aactaacatg ttggcttgcg ggcgttcatg
    10201 ctcagaaaca aggttgggac aagcacttcc aggctaacac agtcagaaat cgaaacgtac
    10261 tctcaacagt tcgcttaggc atggaagttt tgcggcattc tggctacaca ataacaaggg
    10321 aagacttact cgtggctgca accctactag ctcaaaattt attcacacat ggttacgctt
    10381 tggggaaatt atgaggggat ctctcagtac ggggtctgac gctcagtgga acgaaaactc
    10441 acgttaaggg attttggtca tgcaatgtta tggagcagca acgatgttac gcagcagggc
    10501 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
    10561 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
    10621 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
    10681 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
    10741 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
    10801 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
    10861 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
    10921 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
    10981 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
    11041 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
    11101 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
    11161 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
    11221 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
    11281 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat aacaagacga actccaattc
    11341 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    11401 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
    11461 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
    11521 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
    11581 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
    11641 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctgg
//
