LOCUS       sequence-420482-        6135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression of the N-terminal amino acids 1-87 of human 
            C17orf53 N-terminally fused to Profinity eXact-Tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6135)
  TITLE     Pospiech Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPAL7-C17orf53_1-87
FEATURES             Location/Qualifiers
     source          1..6135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    26..50
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             65..87
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(642..660)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      656..703
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(878..883,884..900,901..906))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(933..951)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1019..1123
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1124..1192,1193..1984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1342..1361)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2155..2743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2644..2663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2897..2914
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2929..3068
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2994..3013)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3154..3176
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3170..3361)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4673..5755)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5717..5736
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5756..5833)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     6039..6058
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
       61 taattttgtt taactttaag aaggagatat acatatggga gggaaatcaa acggggaaaa
      121 gaaatatatt gtcgggttca aacagggctt taagagctgc gctaagaagg aggatgtcat
      181 ttctgaaaaa ggcgggaaac tccaaaagtg cttcaaatat gtagacgcag ctagcgctac
      241 attaaacgaa aaagctgtag aagaattgaa aaaagatccg agcgtcgcgt acgtagaaga
      301 agacaagctc ttcaaagctt tgatggcgtg cagtttgcag aagctgtttg ctgtggaaga
      361 ggagtttgaa gatgaggatt tcttgtctgc tgtggaggat gcagagaacc ggtttactgg
      421 ctcactgcct gtgaatgctg ggcgcctgag acctgtctct tctaggccac aggagactgt
      481 gcaggcacag tcctccaggc tgctgctgtt acaccccact gctccctcag aggctttggg
      541 cctgccagac ttggacctct gcctccctgc ctccagcacg ccctgagaat tcctcgaggc
      601 ggccgcataa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      661 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      721 atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
      781 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
      841 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcctatc gatgataagc
      901 tgtcaaacat gagatttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      961 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     1021 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1081 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     1141 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1201 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1261 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1321 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     1381 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     1681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2281 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     2341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2521 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2581 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2641 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2701 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2761 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2821 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2881 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaacagcgcc tgatgcggta
     2941 ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc
     3001 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     3061 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3121 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     3181 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     3241 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     3301 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     3361 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     3421 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     3481 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     3541 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga
     3601 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa
     3661 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
     3721 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc
     3781 agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa
     3841 cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct
     3901 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
     3961 gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct
     4021 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca
     4081 tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc
     4141 aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc
     4201 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
     4261 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta
     4321 gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt
     4381 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
     4441 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc
     4501 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat
     4561 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
     4621 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc
     4681 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4741 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca
     4801 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
     4861 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
     4921 tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact
     4981 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
     5041 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
     5101 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
     5161 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
     5221 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
     5281 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
     5341 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
     5401 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta
     5461 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
     5521 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
     5581 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
     5641 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
     5701 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
     5761 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
     5821 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag
     5881 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
     5941 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
     6001 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
     6061 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
     6121 gagatctcga tcccg
//
