LOCUS       sequence-420483-        8210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Renz C, Asimaki E, Meister C, Albanese V, Petriukov K, Krapoth NC, 
            Wegmann S, Wollscheid HP, Wong RP, Fulzele A, Chen JX, Leon S, 
            Ulrich HD
  TITLE     Ubiquiton-An inducible, linkage-specific polyubiquitylation tool.
  JOURNAL   Mol Cell. 2024 Jan 18;84(2):386-400.e11. doi: 
            10.1016/j.molcel.2023.11.016. Epub 2023 Dec 15.
  PUBMED    38103558
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.11.016"; journalName: "Mol Cell"; date: 
            "2024-01-18- 18"; volume: "84"; issue: "2"; pages: "386-400.e11"
COMMENT     Sequence Label: YIp128-TET-NUbo-E3(48)-VSV
FEATURES             Location/Qualifiers
     source          1..8210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(52..69)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(223..811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(303..322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(982..1773,1774..1842))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1605..1624
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1843..1947)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2015..2033
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2035..2440
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2441..3535
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(3676..3698)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3798..3817
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4011..4033
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4025..4042
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4026..4042
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4052..4285)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             complement(4553..5173)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLKGESWQDFLRNNAKSFRCALLSHRNGAKVHSDTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     5081..5100
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(5253..5277)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5274..5477)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5303..5323)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5478..5857
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     terminator      5969..6156
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6024..6048)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    join(6214..6232,6233..6255,6256..6274,6275..6297,6298..6316,
                     6317..6339,6340..6358,6359..6381,6382..6400,6401..6423,
                     6424..6442,6443..6465,6466..6484)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             6800..6826
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6836..7114
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             7676..7708
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     terminator      7743..7990
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7759..7777)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(8031..8047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8031..8047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8044..8066)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8055..8071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8079..8085,8086..8103,8104..8109))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8124..8145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
       61 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      661 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1261 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1981 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccaactgt
     2041 gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt
     2101 tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg
     2161 aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg
     2221 ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt
     2281 catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg
     2341 ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt
     2401 tcagcaatat atatatatat ttcaaggata taccattcta atgtctgccc ctaagaagat
     2461 cgtcgttttg ccaggtgacc acgttggtca agaaatcaca gccgaagcca ttaaggttct
     2521 taaagctatt tctgatgttc gttccaatgt caagttcgat ttcgaaaatc atttaattgg
     2581 tggtgctgct atcgatgcta caggtgttcc acttccagat gaggcgctgg aagcctccaa
     2641 gaaggctgat gccgttttgt taggtgctgt gggtggtcct aaatggggta caggtagtgt
     2701 tagacctgaa caaggtttac taaaaatccg taaagaactt caattgtacg ccaacttaag
     2761 accatgtaac tttgcatccg actctctttt agacttatct ccaatcaagc cacaatttgc
     2821 taaaggtact gacttcgttg ttgtcagaga attagtggga ggtatttact ttggtaagag
     2881 aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt
     2941 gcaaagaatc acaagaatgg ccgctttcat ggccctacaa catgagccac cattgcctat
     3001 ttggtccttg gataaagcta atgttttggc ctcttcaaga ttatggagaa aaactgtgga
     3061 ggaaaccatc aagaacgaat ttcctacatt gaaggttcaa catcaattga ttgattctgc
     3121 cgccatgatc ctagttaaga acccaaccca cctaaatggt attataatca ccagcaacat
     3181 gtttggtgat atcatctccg atgaagcctc cgttatccca ggttccttgg gtttgttgcc
     3241 atctgcgtcc ttggcctctt tgccagacaa gaacaccgca tttggtttgt acgaaccatg
     3301 ccacggttct gctccagatt tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc
     3361 tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa gaaggtaagg ccattgaaga
     3421 tgcagttaaa aaggttttgg atgcaggtat cagaactggt gatttaggtg gttccaacag
     3481 taccaccgaa gtcggtgatg ctgtcgccga agaagttaag aaaatccttg cttaaaaaga
     3541 ttctcttttt ttatgatatt tgtacataaa ctttataaat gaaattcata atagaaacga
     3601 cacgaaatta caaaatggaa tatgttcata gggtaggccc tttcgtctcg cgcgtttcgg
     3661 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     3721 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     3781 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     3841 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     3901 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     3961 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     4021 acgttgtaaa acgacggcca gtgaattcct acccaccgta ctcgtcaatt ccaagggcat
     4081 cggtaaacat ctgctcaaac tcgaagtcgg ccatatccag agcgccgtag ggggcggagt
     4141 cgtggggggt aaatcccgga cccggggaat ccccgtcccc caacatgtcc agatcgaaat
     4201 cgtctagcgc gtcggcatgc gccatcgcca cgtcctcgcc gtctaagtgg agctcgtccc
     4261 ccaggctgac atcggtcggg ggggccgtcg acagtctgcg cgtgtgtccc gcggggagaa
     4321 aggacaggcg cggagccgcc agccccgcct cttcgggggc gtcgtcgtcc gggagatcga
     4381 gcaggccctc gatggtagac ccgtaattgt ttttcgtacg cgcggtaccg cttacccatc
     4441 tctccgcatc acctttggta aaggttctaa gcttaggtga gaacatccct gcctgaacat
     4501 gagaaaaaac agggtactca tactcacttc taagtgacgg ctgcatacta acagatccac
     4561 tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg aataagaagg
     4621 ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat ggcggcatac
     4681 tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga tcttccaata
     4741 cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taacgcgttc tctagtgaaa
     4801 aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt ttttctgtag
     4861 gccgtgtatc tgaatgtact tttgctccat tgcgatgact tagtaaagca catctaaaac
     4921 ttttagcgtt attgcgtaaa aaatcttgcc agctttcccc ttttaaaggg caaaagtgag
     4981 tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc ttatttttta
     5041 catgccaata cagtgtaggc tgctctacac caagcttctg ggcgagttta cgggttgtta
     5101 aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact ttacttttat
     5161 ctaatctaga catatgaatt ggggccgcgg aggctggatc ggtcccggtg tcttctatgg
     5221 aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa acgagctctg
     5281 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     5341 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     5401 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     5461 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     5521 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     5581 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     5641 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     5701 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     5761 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     5821 attgattact attaataact agtcaataat caatgtcaac atggcggtaa tgttggacat
     5881 gagccaatat aaatgtacat attatgatat ggatacaacg tatgcaatgg gccaagctcc
     5941 tcgagtaatt cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat
     6001 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
     6061 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
     6121 gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt
     6181 aatatagatc aattcctcga tcgagtttac cactccctat cagtgataga gaaaagtgaa
     6241 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     6301 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     6361 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     6421 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     6481 gagaaaagtg aaagtcgagc tcggtaccct atggcatgca tgtgctctgt atgtatataa
     6541 aactcttgtt ttcttctttt ctctaaatat tctttcctta tacattaggt cctttgtagc
     6601 ataaattact atacttctat agacacgcaa acacaaatac acacactaaa ttaccggatc
     6661 aattcggggg atcagtatgc agattttcgt caagactttg accggtaaaa ccgcaacatt
     6721 ggaagttgaa tcttccgata ccatcgacaa cgttaagtcg aaaattcaag acaaggaagg
     6781 gatccctggg tctggggctt acccatacga tgttcctgac tatgcgggga atggtatcct
     6841 ctggcatgag atgtggcatg aaggcctgga agaggcatct cgtttgtact ttggggaaag
     6901 gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat gctatgatgg aacggggccc
     6961 ccagactctg aaggaaacat cctttaatca ggcctatggt cgagatttaa tggaggccca
     7021 agagtggtgc aggaagtaca tgaaatcagg gaatgtcaag gacctcctcc aagcctggga
     7081 cctctattat catgtgttcc gacgaatctc aaagactagt gaattgcaaa gtaatcagca
     7141 tccctcagca caacgcttat ccgctacagg tgtaaacgca cacggacgtc ctcagggctc
     7201 cacgcagaat gccttgagaa ggactggtag agtcaatgga ggtcaccccg tgactactca
     7261 gatggtggaa acagtgcaaa atctagcccc taacttacat cctgagcaaa ttaggtatag
     7321 tttggaaaac acaggctcag tcgaggaaac agtggaaagg tacttgcgtg gtgatgaatt
     7381 cagctttcca cctgggtttg agccctccag ggcgccaatg ggggccaatg cggctgttga
     7441 taataacgct gctggtggcg gagagtttaa cgatcccaga aagaagaaca tgatttgcgc
     7501 tgagaatcta ctcgataaat tccatgtgga tctcaatgaa gatatgagta acttaagctt
     7561 taaggactta gacattgaag agagaaagag attgttggtt tggcaagcca gaaagaattt
     7621 ggagacaaaa ttgcaaagtg ataaagattt gcaaagtttg ctgactttaa ttaattatac
     7681 tgacatagaa atgaacagat tgggaaagta ggcggccgct agggccctgc aggagggccg
     7741 catcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac
     7801 cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat
     7861 gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt
     7921 gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc
     7981 tttaatttgc ggccgtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
     8041 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8101 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8161 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
//
