LOCUS       sequence-42063-e         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PIWIL2
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             564..581
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tctgaatatt ttgtttactt tagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc cggaatgact gtgtgctgga tgtcatgcat
      121 gccatttatc agcagaataa agaacacttc caggatgagt gtactaagct tctggttggc
      181 aatattgtta tcacccgata taacaatcgt acctatcgta ttgatgatgt ggattggaat
      241 aagactccaa aggatagctt cacgatgtct gatgggaaag agatcacatt cttggaatac
      301 tacagcaaaa attatgggat cacagttaag gaagaggacc agccattgct gattcacagg
      361 cccagtgaga gacaggataa tcatgggatg ctgctaaaag gggaaatcct gctgctgcct
      421 gagctttctt ttatgaccgg aatcccagag aagatgaaga aggacttcag agccatgaag
      481 gatttggctc agcaaatcaa tctgagcccc aagcaacacc atagtgcttt ggaatgatga
      541 cgaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaac
//
