LOCUS       sequence-420669-       11048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for agrobacterium mediated 
            transformation. MCS upstream of gateway, driven by COBL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11048)
  AUTHORS   Michniewicz M, Frick EM, Strader LC
  TITLE     Gateway-compatible tissue-specific vectors for plant transformation.
  JOURNAL   BMC Res Notes. 2015 Mar 3;8:63. doi: 10.1186/s13104-015-1010-6.
  PUBMED    25884475
REFERENCE   2  (bases 1 to 11048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2015-03-3"; volume: "8:63. doi"; pages: " 
            10.1186/s13104-015-1010-6"
COMMENT     Sequence Label: pCOBL-GW
FEATURES             Location/Qualifiers
     source          1..11048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    2061..2185
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2210..2215,2216..2233,2234..2240)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2294..2953
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2360..2379)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3295..3600
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3479..3498
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3641..3765)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3818..4525
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(4550..4567)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4550..4566)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4559..4581)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4769..4793
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6094..6723
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7152..8225
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8291..8485
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8721..8743)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8829..8969
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8884..8903
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8984..9001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9155..9743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9235..9254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9830..10624)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tcgcgactca tgtttggttg tactactgtc aatttggtag gattgtgaga ttttgcaaga
     1381 ctgtttattc ctgttgtttt gataaaactt ttacttggtc acctgcaatg caagactagc
     1441 aaagaatact ataaattttg ttctgttttc ttgtatggga cgggcttaaa cgggcctaac
     1501 agtcgttctt tatcggccca ctttaacaac acctaattgt ctagtctagt agtagtattc
     1561 tctagagact ttcaaaaagt ctttggatta ctaaatagta aatgcgaata taagtaaact
     1621 ataaatgtga ataagaatgt ttgaaattga aaaaaagaat tgctagctaa gtgcattaat
     1681 gcttgtgcat taagtcaaat tataacactt ggcacgaaaa aacccatctc atcccccact
     1741 aaagcagcct tcttttggct ttcattaatc tctctgcaca caaaaaccca aaaagctgga
     1801 tgtctccatt ttcaatgaac aattatgagt tcttttttta aaaactcatt atactttgaa
     1861 gattaatcac aaagatttcg aagctaattc gtcatttctt gttactataa tcttttcatt
     1921 gttattaatc actttggctt aagtttctgt atattacgtc gagcttcttc agtcttcatc
     1981 cttgtcttgt cacttgtcag agacagaaga ttaaagaaga agaggcgttt ctctctcttt
     2041 ttttgcttca gctcgagatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     2101 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     2161 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
     2221 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
     2281 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     2341 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     2401 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     2461 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggagt tccgtatggc
     2521 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     2581 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     2641 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     2701 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     2761 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     2821 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     2881 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     2941 gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt atgcgtattt
     3001 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
     3061 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
     3121 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
     3181 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
     3241 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag
     3301 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
     3361 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
     3421 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
     3481 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
     3541 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
     3601 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg
     3661 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
     3721 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgccta ggtgagtcta
     3781 gagagttaat taagacccgg gactagtccc tagagtcctg ctttaatgag atatgcgaga
     3841 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
     3901 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     3961 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     4021 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     4081 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     4141 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     4201 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     4261 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     4321 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     4381 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     4441 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     4501 gcgacaacat gtcgaggctc agcaggacct gcaggcatgc aagcttggca ctggccgtcg
     4561 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4621 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4681 agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt
     4741 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     4801 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     4861 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
     4921 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     4981 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     5041 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     5101 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     5161 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     5221 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     5281 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     5341 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     5401 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     5461 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     5521 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     5581 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     5641 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     5701 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     5761 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     5821 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     5881 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     5941 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     6001 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     6061 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     6121 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     6181 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     6241 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     6301 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     6361 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     6421 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     6481 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     6541 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     6601 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     6661 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     6721 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     6781 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     6841 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     6901 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     6961 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     7021 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     7081 ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc
     7141 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     7201 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     7261 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     7321 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     7381 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     7441 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     7501 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     7561 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     7621 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     7681 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     7741 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     7801 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     7861 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     7921 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     7981 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     8041 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     8101 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     8161 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     8221 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     8281 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     8341 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     8401 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     8461 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     8521 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     8581 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     8641 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
     8701 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     8761 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     8821 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     8881 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     8941 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     9001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     9601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9781 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
     9841 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     9901 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     9961 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
    10021 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
    10081 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
    10141 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
    10201 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
    10261 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
    10321 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    10381 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    10441 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    10501 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    10561 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    10621 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    10681 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    10741 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    10801 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    10861 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
    10921 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
    10981 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
    11041 ctggctgg
//
