LOCUS       sequence-42075-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Mihaylova MM, Vasquez DS, Ravnskjaer K, Denechaud PD, Yu RT, Alvarez
            JG, Downes M, Evans RM, Montminy M, Shaw RJ
  TITLE     Class IIa histone deacetylases are hormone-activated regulators of 
            FOXO and mammalian glucose homeostasis.
  JOURNAL   Cell. 2011 May 13;145(4):607-21.
  PUBMED    21565617
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-05-13"; volume: "145(4)"; pages: "607-21"
COMMENT     Sequence Label: pAd FLAG HDAC5 WT
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             101..124
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(130..154)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             180..221
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(261..280)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    305..353
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tttgctgtcg gtgggggccg agcaggccca ggctgcggca gcccgggaac acagccccag
       61 gccggcagag gagcccatgg agcaggagcc tgccctgggg gattataaag atgatgatga
      121 taaataaata cccagctttc ttgtacaaag tggttgatct agagggcccg cggttcgaag
      181 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag
      241 tttaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
      301 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
      361 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
      421 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
      481 gctcgcagcc aacgtcgggg gcggcaggnc ctgccatagc agatccgatt cgacagatca
      541 ttgaaatgtg tgggcgtggc ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt
      601 agttttgtat ctgttttgca gcagccgccg ccgccatgag caccaactcg tttgatggaa
      661 gcattgtgag ctcatatttg acaacgcgca tgcccnatgg ccggggtgcg tcagaatgtg
      721 atgggctcca gcattgatgg tcgccccgtc ctgcccgcaa actctact
//
