LOCUS       sequence-42133-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS
  TITLE     An improved reverse genetics system for mammalian orthoreoviruses.
  JOURNAL   Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29.
  PUBMED    20042210
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2010-03-15"; volume: "398(2)"; pages: "194-200. Epub 2009 Dec
            29"
COMMENT     Sequence Label: pT7-M1-S1-S2-S4T1L
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(324..342)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      338..385
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgatcagtat aatcaggcta atccgaattc tacgatgttc cgtacgaagc catttacgaa
       61 tgcgcaatgg ggacgaggaa ataccggagc gactagtgcc gcaattgcag cccttatcta
      121 atcgtcttgg agtgagggga tccccccaca cccctcacga ctgaccacac attcatcggg
      181 tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga ggacgtcgtc
      241 cactcggatg gctaagggag agctcaaaaa aaaggatccg gctgctaaca aagcccgaaa
      301 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      361 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat cgagatcctc
      421 tagataatac gactcactat agctattcgc gcctatggat gcatctctca ttacagagat
      481 acggaaaata gtactccaac tatctgtatc aagcaatggc tcccagtcaa aagaaatcga
      541 ggaaatcaag aaacaagtcc aggtcaacgt tgatgatatc agggctgcca atattaaact
      601 cgacggactt ggaagacaga ttgctgacat cagcaatagc atctcaacca ttgagtcaag
      661 attgggtgag atggataatc gacttgtggg tatctcgagt caggtcacgc aattatctaa
      721 ctcagttagc cagaacactc agagcatatc ctcattgggt gacagaatca atgctgtcga
//
