LOCUS       sequence-421551-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP in response to 4'-O-Methylnorbelladine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   d'Oelsnitz S, Diaz DJ, Kim W, Acosta DJ, Dangerfield TL, Schechter 
            MW, Minus MB, Howard JR, Do H, Loy JM, Alper HS, Zhang YJ, Ellington
            AD
  TITLE     Biosensor and machine learning-aided engineering of an 
            amaryllidaceae enzyme.
  JOURNAL   Nat Commun. 2024 Mar 7;15(1):2084. doi: 10.1038/s41467-024-46356-y.
  PUBMED    38453941
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-03-7"; volume: "15(1):2084. doi"; pages: " 
            10.1038/s41467-024-46356-y"
COMMENT     Sequence Label: pSens-4OmeNB
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             724..741
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggcatcca agggcgagga gctctttact ggcgtagtac caattctcgt agagctcgat
       61 ggcgatgtaa atggccataa gttttccgta cgcggcgagg gcgagggcga tgcaactaac
      121 ggcaagctca ctctcaagtt tatttgtact actggcaagc tcccagtacc atggccaact
      181 ctcgtaacta ctctgaccta tggcgtacaa tgtttttccc gctatccaga tcacatgaag
      241 caacatgatt tttttaagtc cgcaatgcca gagggctatg tacaagagcg cactattagc
      301 tttaaggatg atggcaccta taagactcgc gcagaggtaa agtttgaggg cgatactctc
      361 gtaaatcgca ttgagctcaa gggcattgat tttaaggagg atggcaatat tctcggccat
      421 aagctggagt ataatttcaa ttcccataat gtatatatta ccgcagataa gcaaaagaat
      481 ggcattaagg cgaattttaa gattcgccat aatgtggagg atggctccgt acaactcgca
      541 gatcattatc aacaaaatac tccaattggc gatggcccag tactcctccc agataatcat
      601 tatctctcca ctcaatccgt gctctccaaa gatccaaatg agaagcgcga tcacatggta
      661 ctcctggagt ttgtaactgc agcaggcatt actcatggca tggatgagct ctataagctc
      721 gagcaccacc accaccacca ctga
//
