LOCUS       sequence-421557-        2859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 5 homology arm for Golden Gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, 
            Barrick JE
  TITLE     Single-step genome engineering in the bee gut symbiont Snodgrassella
            alvi
  JOURNAL   bioRxiv 2023.09.19.558440
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.19.558440"
COMMENT     Sequence Label: pBTK1059
FEATURES             Location/Qualifiers
     source          1..2859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1264..1852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1344..1363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2034..2693
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2100..2119)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2764..2798
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ggtctcatgt agaattctgg atactgtctg gttatctaat cagggggctc tcggacaata
       61 cgatacatca atttccacta ttgctcatca ggcaaatcag gtgacttttc tgaaaacaac
      121 cggatacgat tttggtatac gcagctttga tgatcagctt attgcaccgc tgcaaaaagt
      181 tttaaatcag ccactgcctg cgaatcggtc acggctgatt gcgctgcata tcataggttc
      241 tcatcctaat ccgtgcgacc gtcttccaca accagcgcat cactatgtcg ataataacaa
      301 cagtaactgc tatattgaca ctatccggca gacagacaat ttactaagtc agatatatag
      361 tgcattacag cacagcggtc agccattcag tatgttgtat ttcagcgatc acggtttaag
      421 tcacgataaa aacagttatg aaaaaaacag cctcgtgcgt tctgaaagta ttcttcgtca
      481 taatgatcac ttctatcaga actaccacgt accattagtg attatcagca gcaatgaaac
      541 cggacaatta cgtaacaaag cacggcgcag cggtttagaa ttgctggatg gagttgcaca
      601 atggctggga atcagcaccc cgcaactgcc gtatagtaaa caatttttca gtgaaacgga
      661 cagtactcag ctagaggttc tggatctcaa tcagcggtta caacctgtag ataagctgca
      721 aaacgatcct ctccctgcac aattgcagtg acaattacat aaaaaaacaa tctctctgtt
      781 tttttacaaa gcagagagat taaatttttc aaataatttc agattaaagc tttacatttg
      841 atatgaatca aaacacttct gaacaaaaac attataatga atttactttg attggtttcc
      901 tcttgatgtg tgtgtttaag tgtggctctt cgccacactt ttttttatct taattttgac
      961 attcttaaca taattattca attgttactg attcaaattt gttctgatca gcttcctgag
     1021 aatccgcaaa atccagtact acttcatctg actgccagct gagaccgact ttcagccaaa
     1081 aaacttaaga ccgccggtct tgtccactac cttgcagtaa tgcggtggac aggatcggcg
     1141 gttttctttt ctcttctcaa acgtacggtt atccacagaa tcaggggata acgcaggaaa
     1201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1681 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1921 ggtcatgatt gatcgggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     1981 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
     2041 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     2101 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     2161 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     2221 ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg
     2281 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     2341 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     2401 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     2461 ttttcgtttc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     2521 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     2581 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     2641 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taatttgata
     2701 tcgagctcgc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
     2761 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtcctttggt cgaaaaaaaa
     2821 agcccgcact gtcaggtgtg ggcttttttc tgtgtttcc
//
