LOCUS       sequence-421604-        2795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 5 homology arm for Golden Gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, 
            Barrick JE
  TITLE     Single-step genome engineering in the bee gut symbiont Snodgrassella
            alvi
  JOURNAL   bioRxiv 2023.09.19.558440
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.19.558440"
COMMENT     Sequence Label: pBTK1051
FEATURES             Location/Qualifiers
     source          1..2795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(102..136)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(207..866)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     781..800
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1048..1636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1537..1556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgggtaccg gttactccgt taagaattct acatgagacc ggaaacacag aaaaaagccc
       61 acacctgaca gtgcgggctt tttttttcga ccaaaggacc aataaaaaac gcccggcggc
      121 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
      181 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
      241 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
      301 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
      361 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
      421 ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
      481 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
      541 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
      601 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
      661 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
      721 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
      781 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
      841 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
      901 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
      961 tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1021 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1081 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1141 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1201 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1261 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1321 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1381 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1441 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1501 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1561 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1621 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1681 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtacgtt
     1741 tgagaagaga aaagaaaacc gccgatcctg tccaccgcat tactgcaagg tagtggacaa
     1801 gaccggcggt cttaagtttt ttggctgaaa gtcggtctca gctggctgca ccagtaacgc
     1861 tgattatctg gctctagaaa aaactcaaat accaaaattt atcagacacg gataaaaatg
     1921 attaaacgta ttgtaaatat aaatacctta tcgaataaat gcagatgact aatgttctga
     1981 aaaaagataa gctacaatga actatcacaa accgatgcaa tgctcaggga gccggcatga
     2041 ccctgtttct acccttgatt tacctgctct gtggtctgat cgccggcaaa ttaacccggg
     2101 atacaaaacc tgttttgtct ctgctactga caaaatttat tatccctgtc gtaattatct
     2161 ataatatagc gaccaatttc agcaatatgg gcgcggtatt aattaccact attgttgtta
     2221 tgagcataat gctgcttgcc ggtaaatttc tccggatcaa tccggtaagc accctgtgtt
     2281 tcagctatct gaatattggc tggctcggtc ttcccatagc agcctcactc tttggtaatc
     2341 aggctgccgc aatatttata tctgcctata tcggcagttc tattgtcggt aactcacttg
     2401 gggcatggct gcttaccggc atccggttca atatcagaaa actgattcaa atgcctcccg
     2461 tcattgccct tattattggc tgcttattgt taccactggg taaacaaatc cgcgagctgg
     2521 atataattta ttccctcgct aaatttctga tgagctttct tggcatggct attcttggta
     2581 tgtggctggc taaaagcaga attacactga ccgacatcag aaacaccatt cagaccttcc
     2641 tgaaaaaagc tgcggcttat ttctgcatcc tctccgccct gcttggcctt gcctgtctgt
     2701 gccaacagaa ccttattctg gaaaatagtg ccgcacttta cttattttgc ctgcttcctc
     2761 cggcagccaa tatcattgtt cttgaaaccc actat
//
