LOCUS       sequence-42175-e        4526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mos1 transposase expression vector for use in creating targeted 
            transgene insertions and targeted deletions in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ601 - Peft-3 Mos1 transposase
FEATURES             Location/Qualifiers
     source          1..4526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1549..1571
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1568..1584
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1568..1584
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1604..1624
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1625..2224
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    2233..2257
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3350..3374)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3379..3702
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(3706..3726)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3734..3751)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3734..3750)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3743..3765)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3959..3978)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4078..4100
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4138..4156)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4224..4328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4329..4397,4398..4526,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccag cggataacaa
     1561 tttcacacag gaaacagcta tgaccatgat tacgccaagc tatcaacttt gtatagaaaa
     1621 gttggcacct ttggtctttt attgtcaact tccattggtt cttccattgt ttctgttaaa
     1681 ttaatgaatt tttcataaaa taaagacatt atacaatata aaaatgaaga atttattgaa
     1741 aataaactgc cagagagaaa aagtatgcaa cactcccgcc gagagtgttt gaaatggtgt
     1801 acggtacatt ttcgtgctag gagttagatg tgcaggcagc aacgagaggg ggagagattt
     1861 ttttgggcct tgtgaaatta acgtgagttt tctggtcatc tgactaatca tgttggtttt
     1921 ttgttggttt attttgtttt tatctttgtt tttatccaga ttaggaaatt taaattttat
     1981 gaatttataa tgaggtcaaa cattcagtcc cagcgttttt cctgttctca ctgtttagtc
     2041 gaatttttat tttaggcttt caacaaatgt tctaactgtc ttatttgtga cctcactttt
     2101 tatatttttt taatttttaa aaatattaga agtttctagg ataatttttt cgacttttat
     2161 tctctctacc gtccgcactc ttcttacttt taaattaaat tgtttttttt tcagttggga
     2221 aacactttgc tcacaagttt gtacaaaaaa gcaggcttaa tgtcgagttt cgtgccgaat
     2281 aaagagcaaa cgcggacagt attaattttc tgttttcatt tgaagaaaac agctgcggaa
     2341 tcgcaccgaa tgcttgttga agcctttggc gaacaagtac caactgtgaa aacgtgtgaa
     2401 cggtggtttc aacgcttcaa aagtggtgat tttgacgtcg acgacaaaga gcacggaaaa
     2461 ccgccaaaaa ggtacgaaga cgccgaactg caagcattat tggatgaaga cgatgctcaa
     2521 acgtaagttt aaacatatat actaactaac ccatggatta tttaaatttt caggcaaaaa
     2581 caactcgcag agcagttgga agtaagtcaa caagcagttt ccaatcgctt gcgagagatg
     2641 ggaaagattc agaaggtcgg tagatgggtg ccacatgagt tgaacgagag gcagatggag
     2701 aggcgcaaaa acacatgcga aattttgctt tcacgataca aaaggaagtc gtttttgcat
     2761 cgtatcgtta ctggagatga aaaatggatc ttttttgtta atcctaaacg taaaaagtca
     2821 tacgttgatc ctggacaacc ggccacatcg actgctcgac cgaatcgctt tggcaagaag
     2881 acgatgctct gtgtttggtg ggatcagagc ggtgtcattt actatgagct cttgaaaccc
     2941 ggcgaaacgg tgaatacggc acgctaccaa caacaattga tcaatttgaa ccgtgcgctt
     3001 cagagaaaac gaccggaata tcaaaaaaga caacacaggg tcatttttct ccatgacaac
     3061 gctccatcac atacggcaag agcggttcgc gacacgttgg aaacactcaa ttgggaagtg
     3121 cttccgcatg cggcttactc accagacctg gccccatccg attaccacct attcgcttcg
     3181 atgggacacg cactcgctga gcagcgcttc gattcttacg aaagtgtgaa aaaatggctc
     3241 gatgaatggt tcgccgcaaa agacgatgag ttctactggc gtggaatcca caaattgccc
     3301 gagagatggg aaaaatgtgt cgcgagcgac ggcaaatact ttgaatagta cccagctttc
     3361 ttgtacaaag tgggataaat gcaaaatcct ttcaagcatt cccttcttct ctatcactct
     3421 tctttctttt tgtcaaaaaa ttctctcgct aatttatttg cttttttaat gttattattt
     3481 tatgactttt tatagtcact gaaaagtttg catctgagtg aagtgaatgc tatcaaaatg
     3541 tgattctgtc tgatgtactt tcacaatctc tcttcaattc cattttgaag tgctttaaac
     3601 ccgaaaggtt gagaaaaatg cgagcgctca aatatttgta ttgtgttcgt tgagtgaccc
     3661 aacaaaaaga ggaaacttta ttgtgccgcc aagaaaaaag tctcacaact ttattataca
     3721 tagttgataa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3781 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3841 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     3901 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     3961 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     4021 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     4081 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     4141 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4201 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4261 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4321 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4381 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4441 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4501 ttcgccccga agaacgtttt ccaatg
//
