LOCUS       sequence-421764-        1740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to
            be used in the yeast secretion/display (YSD) extension of the MoClo 
            yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD005
FEATURES             Location/Qualifiers
     source          1..1740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(105..139)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(210..869)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     784..803
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1051..1639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1540..1559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgatgtctg cattatttaa ttttcgttct cttctgcaag tgatattgtt
       61 actgatttgt tcatgttctt atgttcatgc ttgagaccag accaataaaa aacgcccggc
      121 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
      181 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
      241 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
      301 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
      361 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
      421 cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
      481 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
      541 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
      601 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
      661 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
      721 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
      781 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
      841 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
      901 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
      961 acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1021 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1081 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1141 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1201 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1261 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1321 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1381 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1441 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1501 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1561 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1621 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1681 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag
//
