LOCUS       sequence-421791-        1728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes S. cerevisiae PLB1 pre- signal peptide as a Type 3a' part to
            be used in the yeast secretion/display (YSD) extension of the MoClo 
            yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD008
FEATURES             Location/Qualifiers
     source          1..1728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      707..741
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(935..1523)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1015..1034)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1705..1728,1..636)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
       61 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
      121 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
      181 gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
      241 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
      301 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
      361 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ttcagccaat
      421 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
      481 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
      541 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
      601 caacagtact gcgatgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact
      661 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
      721 ttgccgccgg gcgtttttta ttggtctggt ctcaagcatt aacgttttct gttagagaag
      781 tcaaaactgc agcagaaacc aacaaactct gcaacttcat catatgagac cgactacggt
      841 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      901 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      961 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1021 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1081 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1141 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1201 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1261 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1321 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1381 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1441 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1501 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1561 agtggaacga aaactcacgt taagggattt tggtcatgat tgatcgggca cgtaagaggt
     1621 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1681 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatat
//
