LOCUS       sequence-421833-         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and
            linker (residues 42-203, codon-optimized for insect cell expression,
            with a C-terminal His tag) in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Bruguera ES, Mahoney JP, Weis WI
  TITLE     The co-receptor Tspan12 directly captures Norrin to promote 
            ligand-specific beta-catenin signaling
  JOURNAL   bioRxiv 2024.02.03.578714
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.02.03.578714"
COMMENT     Sequence Label: mFzd4CRDL(42-203)-6xHis_pAcGP67a
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             complement(213..224)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             559..576
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggtaagcg ctattgtttt atatgtgctt ttggcggcgg cggcgcattc tgcctttgcg
       61 gcggatcccg agcgccgctg cgacccaatc cgcattgcca tgtgccagaa cctgggatac
      121 aatgtgacca agatgccaaa cctggtgggc cacgagctgc agacagatgc cgagctgcag
      181 ctgacaacct tcacccccct gatccagtac ggctgcagct cccagctgca gttctttctg
      241 tgcagcgtgt atgtgccgat gtgcaccgag aagatcaaca ttccaatcgg accatgcgga
      301 ggaatgtgcc tgtccgtgaa gcgccgctgc gagccagtgc tgcgcgagtt cggctttgcc
      361 tggcccgata ccctgaattg ctcgaagttc cccccgcaga acgatcacaa tcatatgtgc
      421 atggagggac caggagacga ggaggtgccg ctgccacaca agacgccaat ccagcccgga
      481 gaggagtgcc attcggtggg ctccaactcg gaccagtaca tttgggtgaa gcgcagcctg
      541 aattgcgtgc tgaagggaca tcatcatcat catcat
//
