LOCUS       sequence-421877-        9841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Peroxidase (APEX) reporter for beta 2-adrenergic receptor (beta-2AR)
            and beta-arrestin 2 interaction (component 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Zhou G, Wan WW, Wang W
  TITLE     Modular Peroxidase-Based Reporters for Detecting Protease Activity 
            and Protein Interactions with Temporal Gating.
  JOURNAL   J Am Chem Soc. 2022 Dec 21;144(50):22933-22940. doi: 
            10.1021/jacs.2c08280. Epub 2022 Dec 13.
  PUBMED    36511757
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.2c08280"; journalName: "J Am Chem Soc"; date: 
            "2022-12-21- 21"; volume: "144"; issue: "50"; pages: "22933-22940"
COMMENT     Sequence Label: beta-2AR-PPI-CALPon1.0 (APEX)
FEATURES             Location/Qualifiers
     source          1..9841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1847..1964
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2081..2384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2385..2588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2539..2559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(4333..4335,4336..5046)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4333..4335,4336..5046)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4375..4396)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4636..4655)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4983..5004
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5698..6286
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5751..5771)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6169..6180)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6358..6591
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6669..6790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6706..6725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6760..6779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6830..6965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6985..7004)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6986..7004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7014..7031)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7014..7030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7023..7045)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7172..7627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7259..7278)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7469..7490
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7653..7757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7758..7826,7827..8618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7976..7995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8789..9377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9278..9297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9531..9548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9665..9686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9701..9706,9707..9724,9725..9731)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9739..9755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9744..9766
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9763..9779
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9763..9779
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9798..9818
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9800..9818
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagagcg ctgccgtctg gaccgatggc tgtgtggaag
     1741 tgctcgccga tagtggaaac agacgcccca gcactcgtcc tagggcaaag gatctgcagt
     1801 aatgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     1861 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1921 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1981 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     2041 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2101 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2161 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2221 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2281 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2341 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2401 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2461 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2521 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2581 gcagagctct ctggctaact gtcgggatcc accatggggc aacccgggaa cggcagcgcc
     2641 ttcttgctgg cacccaatag aagccatgcg ccggaccacg acgtcacgca gcagagggac
     2701 gaggtgtggg tggtgggcat gggcatcgtc atgtctctca tcgtcctggc catcgtgttt
     2761 ggcaatgtgc tggtcatcac agccattgcc aagttcgagc gtctgcagac ggtcaccaac
     2821 tacttcatca cttcactggc ctgtgctgat ctggtcatgg gcctggcagt ggtgcccttt
     2881 ggggccgccc atattcttat gaaaatgtgg acttttggca acttctggtg cgagttttgg
     2941 acttccattg atgtgctgtg cgtcacggcc agcattgaga ccctgtgcgt gatcgcagtg
     3001 gatcgctact ttgccattac ttcacctttc aagtaccaga gcctgctgac caagaataag
     3061 gcccgggtga tcattctgat ggtgtggatt gtgtcaggcc ttacctcctt cttgcccatt
     3121 cagatgcact ggtaccgggc cacccaccag gaagccatca actgctatgc caatgagacc
     3181 tgctgtgact tcttcacgaa ccaagcctat gccattgcct cttccatcgt gtccttctac
     3241 gttcccctgg tgatcatggt cttcgtctac tccagggtct ttcaggaggc caaaaggcag
     3301 ctccagaaga ttgacaaatc tgagggccgc ttccatgtcc agaaccttag ccaggtggag
     3361 caggatgggc ggacggggca tggactccgc agatcttcca agttctgctt gaaggagcac
     3421 aaagccctca agacgttagg catcatcatg ggcactttca ccctctgctg gctgcccttc
     3481 ttcatcgtta acattgtgca tgtgatccag gataacctca tccgtaagga agtttacatc
     3541 ctcctaaatt ggataggcta tgtcaattct ggtttcaatc cccttatcta ctgccggagc
     3601 ccagatttca ggattgcctt ccaggagctt ctgtgcctgc gcaggtcttc tttgaaggcc
     3661 tatgggaatg gctactccag caacggcaac acaggggagc agagtggata tcacgtggaa
     3721 caggagaaag aaaataaact gctgtgtgaa gacctcccag gcacggaaga ctttgtgggc
     3781 catcaaggta ctgtgcctag cgataacatt gattcacaag ggaggaattg tagtacaaat
     3841 gactcactgc tggagctggc cgagaagctg gccggcctgg acatcaacgg tggagctagc
     3901 atggaagtca gcgccttgga gaaggaggtc tctgcccttg aaaaagaggt ttcagccctc
     3961 gagaaagaag tctctgcgct cgaaaaagaa gtgtcagcgc ttgagaagga gaacctgtac
     4021 ttccagaaca ccggtgggct gctgcagctg cccagcgaca aagccctgct gtccgatccc
     4081 gtgttcagac ctctggtcga taagtatgca gccgacgagg atgctttttt cgcagattac
     4141 gcagaagcac atcagaagct gtcagaactg ggatttgccg acgccgctag caaagtttcc
     4201 gccctcaagg aaaaggtcag tgctcttaaa gagaaagtat ccgcactcaa agagaaggtt
     4261 agcgcactga aagaaaaggt cagcgcactg aaagaaggat cctctggttc cggaagtggc
     4321 ggaggagggt cagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4381 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4441 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     4501 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     4561 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4621 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4681 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4741 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     4801 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     4861 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4921 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4981 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     5041 tacaagtccg gtggctctgg gagctctagt ggatcgacta gtgggaaatc atacccaaca
     5101 gtgtccgcag actaccagga tgccgtggag aaagccaaga agaggctggg agggtttatc
     5161 gcagaaaaga ggtgcgcacc tctgatgctg agactggctt tccacagcgc aggcaccttt
     5221 gacaagcgaa ccaaaacagg cggacccttt ggaacaatcc ggcgccctgc tgaactggca
     5281 catagtgcca acagtgggct ggacatcgcc gtgcggctgc tggaacctct gaaagcagag
     5341 ttcccaattc tgtcctacgc cgatttttat cagctggcag gagtggtcgc tgtggaggtc
     5401 actgggggcc ccaaggtgcc tttccaccca ggacgggagg acaaaccaga accacctcca
     5461 gaggggcgcc tgccagatcc gacaaagggc tccgaccatc tgcgagatgt gtttgggaaa
     5521 gctatgggcc tgactgacca ggatatcgtc gcactgtctg gagggcacac ccttggcgcc
     5581 gctcataagg aaaggtcagg cttcgaggga ccctggacaa gcaaccccct gattttcgac
     5641 aattcttact ttactgaact gctgagtgga gagaaggaat aaacgcgtta agtcgacaat
     5701 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5761 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5821 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5881 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5941 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6001 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6061 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6121 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6181 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6241 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     6301 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6361 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6421 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6481 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6541 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     6601 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6661 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6781 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6841 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6901 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6961 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     7021 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7081 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7141 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     7201 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7261 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7321 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7381 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7441 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7501 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7561 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7621 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7681 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7741 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7801 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7861 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7921 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7981 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8041 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8101 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8161 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8221 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8281 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8341 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8401 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8461 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8521 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8581 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8761 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8821 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8881 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8941 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9001 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9061 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9121 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9181 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9241 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9301 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9361 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9421 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9481 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9541 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9601 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9661 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9721 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     9781 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     9841 c
//
