LOCUS       sequence-421908-       13254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  activating MTOR mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 13254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pcDNA3-FLAG-MTOR-L1460P
FEATURES             Location/Qualifiers
     source          1..13254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(83..103)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        88..417
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      268..403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     330..349
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             484..1278
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(538..557)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1148..1167
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1452..1573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1489..1508)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1543..1562
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1622..1638)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1622..1638)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1635..1657)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1646..1662
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1670..1676,1677..1694,1695..1700))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1715..1736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1853..1870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2024..2612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2104..2123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2783..3574,3575..3643))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3406..3425
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3644..3748)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3823..3842)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4014..4393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4394..4597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4548..4568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4642..4661
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4642..4660
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4746..4755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7627..7636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10836..11114
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             10848..11120
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     primer_bind     complement(12535..12551)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12535..12551)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(12586..12604)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12587..12604)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(12624..12641)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12630..12854
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(12900..13254,1..74)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12987..13006)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13197..13218
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
       61 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      121 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      181 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      241 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      301 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
      361 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      421 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
      481 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
      541 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
      601 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
      661 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
      721 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
      781 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
      841 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
      901 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
      961 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     1021 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     1081 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     1141 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     1201 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     1261 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     1321 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     1381 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     1441 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1501 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1561 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     1621 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1681 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1741 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1801 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1861 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1921 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1981 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2041 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2101 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2161 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2221 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2281 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2341 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2401 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2461 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2521 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2581 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2641 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2701 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2761 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2821 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2881 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2941 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3001 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3061 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3121 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3181 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3241 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3301 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3361 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3421 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3481 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3541 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3601 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3661 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3721 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     3781 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     3841 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     3901 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     3961 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     4021 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     4081 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     4141 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     4201 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     4261 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     4321 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     4381 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     4441 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     4501 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     4561 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     4621 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag cttggtaccg
     4681 agctcggatc cactagtaac ggccgccagt gtgctggaat tctgcagata tccatcacac
     4741 tggcggccgc catggattat aaagatgatg atgatatgct tggaaccgga cctgccgccg
     4801 ccaccaccgc tgccaccaca tctagcaatg tgagcgtcct gcagcagttt gccagtggcc
     4861 taaagagccg gaatgaggaa accagggcca aagccgccaa ggagctccag cactatgtca
     4921 ccatggaact ccgagagatg agtcaagagg agtctactcg cttctatgac caactgaacc
     4981 atcacatttt tgaattggtt tccagctcag atgccaatga gaggaaaggt ggcatcttgg
     5041 ccatagctag cctcatagga gtggaaggtg ggaatgccac ccgaattggc agatttgcca
     5101 actatcttcg gaacctcctc ccctccaatg acccagttgt catggaaatg gcatccaagg
     5161 ccattggccg tcttgccatg gcaggggaca cttttaccgc tgagtacgtg gaatttgagg
     5221 tgaagcgagc cctggaatgg ctgggtgctg accgcaatga gggccggaga catgcagctg
     5281 tcctggttct ccgtgagctg gccatcagcg tccctacctt cttcttccag caagtgcaac
     5341 ccttctttga caacattttt gtggccgtgt gggaccccaa acaggccatc cgtgagggag
     5401 ctgtagccgc ccttcgtgcc tgtctgattc tcacaaccca gcgtgagccg aaggagatgc
     5461 agaagcctca gtggtacagg cacacatttg aagaagcaga gaagggattt gatgagacct
     5521 tggccaaaga gaagggcatg aatcgggatg atcggatcca tggagccttg ttgatcctta
     5581 acgagctggt ccgaatcagc agcatggagg gagagcgtct gagagaagaa atggaagaaa
     5641 tcacacagca gcagctggta cacgacaagt actgcaaaga tctcatgggc ttcggaacaa
     5701 aacctcgtca cattaccccc ttcaccagtt tccaggctgt acagccccag cagtcaaatg
     5761 ccttggtggg gctgctgggg tacagctctc accaaggcct catgggattt gggacctccc
     5821 ccagtccagc taagtccacc ctggtggaga gccggtgttg cagagacttg atggaggaga
     5881 aatttgatca ggtgtgccag tgggtgctga aatgcaggaa tagcaagaac tcgctgatcc
     5941 aaatgacaat ccttaatttg ttgccccgct tggctgcatt ccgaccttct gccttcacag
     6001 atacccagta tctccaagat accatgaacc atgtcctaag ctgtgtcaag aaggagaagg
     6061 aacgtacagc ggccttccaa gccctggggc tactttctgt ggctgtgagg tctgagttta
     6121 aggtctattt gcctcgcgtg ctggacatca tccgagcggc cctgccccca aaggacttcg
     6181 cccataagag gcagaaggca atgcaggtgg acgccacagt cttcacttgc atcagcatgc
     6241 tggctcgagc aatggggcca ggcatccagc aggatatcaa ggagctgctg gagcccatgc
     6301 tggcagtggg actaagccct gccctcactg cagtgctcta cgacctgagc cgtcagattc
     6361 cacagctaaa gaaggacatt caagatgggc tactgaaaat gctgtccctg gtccttatgc
     6421 acaaacccct tcgccaccca ggcatgccca agggcctggc ccatcagctg gcctctcctg
     6481 gcctcacgac cctccctgag gccagcgatg tgggcagcat cactcttgcc ctccgaacgc
     6541 ttggcagctt tgaatttgaa ggccactctc tgacccaatt tgttcgccac tgtgcggatc
     6601 atttcctgaa cagtgagcac aaggagatcc gcatggaggc tgcccgcacc tgctcccgcc
     6661 tgctcacacc ctccatccac ctcatcagtg gccatgctca tgtggttagc cagaccgcag
     6721 tgcaagtggt ggcagatgtg cttagcaaac tgctcgtagt tgggataaca gatcctgacc
     6781 ctgacattcg ctactgtgtc ttggcgtccc tggacgagcg ctttgatgca cacctggccc
     6841 aggcggagaa cttgcaggcc ttgtttgtgg ctctgaatga ccaggtgttt gagatccggg
     6901 agctggccat ctgcactgtg ggccgactca gtagcatgaa ccctgccttt gtcatgcctt
     6961 tcctgcgcaa gatgctcatc cagattttga cagagttgga gcacagtggg attggaagaa
     7021 tcaaagagca gagtgcccgc atgctggggc acctggtctc caatgccccc cgactcatcc
     7081 gcccctacat ggagcctatt ctgaaggcat taattttgaa actgaaagat ccagaccctg
     7141 atccaaaccc aggtgtgatc aataatgtcc tggcaacaat aggagaattg gcacaggtta
     7201 gtggcctgga aatgaggaaa tgggttgatg aactttttat tatcatcatg gacatgctcc
     7261 aggattcctc tttgttggcc aaaaggcagg tggctctgtg gaccctggga cagttggtgg
     7321 ccagcactgg ctatgtagta gagccctaca ggaagtaccc tactttgctt gaggtgctac
     7381 tgaattttct gaagactgag cagaaccagg gtacacgcag agaggccatc cgtgtgttag
     7441 ggcttttagg ggctttggat ccttacaagc acaaagtgaa cattggcatg atagaccagt
     7501 cccgggatgc ctctgctgtc agcctgtcag aatccaagtc aagtcaggat tcctctgact
     7561 atagcactag tgaaatgctg gtcaacatgg gaaacttgcc tctggatgag ttctacccag
     7621 ctgtgtccat ggtggccctg atgcggatct tccgagacca gtcactctct catcatcaca
     7681 ccatggttgt ccaggccatc accttcatct tcaagtccct gggactcaaa tgtgtgcagt
     7741 tcctgcccca ggtcatgccc acgttcctta atgtcattcg agtctgtgat ggggccatcc
     7801 gggaattttt gttccagcag ctgggaatgt tggtgtcctt tgtgaagagc cacatcagac
     7861 cttatatgga tgaaatagtc accctcatga gagaattctg ggtcatgaac acctcaattc
     7921 agagcacgat cattcttctc attgagcaaa ttgtggtagc tcttgggggt gaatttaagc
     7981 tctacctgcc ccagctgatc ccacacatgc tgcgtgtctt catgcatgac aacagcccag
     8041 gccgcattgt ctctatcaag ttactggctg caatccagct gtttggcgcc aacctggatg
     8101 actacctgca tttactgctg cctcctattg ttaagttgtt tgatgcccct gaagctccac
     8161 tgccatctcg aaaggcagcg ctagagactg tggaccgcct gacggagtcc ctggatttca
     8221 ctgactatgc ctcccggatc attcacccta ttgttcgaac actggaccag agcccagaac
     8281 tgcgctccac agccatggac acgctgtctt cacttgtttt tcagctgggg aagaagtacc
     8341 aaattttcat tccaatggtg aataaagttc tggtgcgaca ccgaatcaat catcagcgct
     8401 atgatgtgct catctgcaga attgtcaagg gatacacact tgctgatgaa gaggaggatc
     8461 ctttgattta ccagcatcgg atgcttagga gtggccaagg ggatgcattg gctagtggac
     8521 cagtggaaac aggacccatg aagaaactgc acgtcagcac catcaacctc caaaaggcct
     8581 ggggcgctgc caggagggtc tccaaagatg actggctgga atggctgaga cggctgagcc
     8641 tggagctgct gaaggactca tcatcgccct ccctgcgctc ctgctgggcc ctggcacagg
     8701 cctacaaccc gatggccagg gatctcttca atgctgcatt tgtgtcctgc tggtctgaac
     8761 tgaatgaaga tcaacaggat gagctcatca gaagcatcga gttggccctc acctcacaag
     8821 acatcgctga agtcacacag accctcttaa acttggctga attcatggaa cacagtgaca
     8881 agggccccct gccactgaga gatgacaatg gcattgttct gctgggtgag agagctgcca
     8941 agtgccgagc atatgccaaa gcactacact acaaagaact ggagttccag aaaggcccca
     9001 cccctgccat tctagaatct ctcatcagca ttaataataa gctacagcag ccggaggcag
     9061 cggccggagt gttagaatat gccatgaaac actttggaga gctggagatc caggctacct
     9121 ggtatgagaa actgcacgag tgggaggatg cccctgtggc ctatgacaag aaaatggaca
     9181 ccaacaagga cgacccagag ctgatgctgg gccgcatgcg ctgcctcgag gccttggggg
     9241 aatggggtca actccaccag cagtgctgtg aaaagtggac cctggttaat gatgagaccc
     9301 aagccaagat ggcccggatg gctgctgcag ctgcatgggg tttaggtcag tgggacagca
     9361 tggaagaata cacctgtatg atccctcggg acacccatga tggggcattt tatagagctg
     9421 tgctggcact gcatcaggac ctcttctcct tggcacaaca gtgcattgac aaggccaggg
     9481 acctgctgga tgctgaatta actgcaatgg caggagagag ttacagtcgg gcatatgggg
     9541 ccatggtttc ttgccacatg ctgtccgagc tggaggaggt tatccagtac aaacttgtcc
     9601 ccgagcgacg agagatcatc cgccagatct ggtgggagag actgcagggc tgccagcgta
     9661 tcgtagagga ctggcagaaa atccttatgg tgcggtccct tgtggtcagc cctcatgaag
     9721 acatgagaac ctggctcaag tatgcaagcc tgtgcggcaa gagtggcagg ctggctcttg
     9781 ctcataaaac tttagtgttg ctcctgggag ttgatccgtc tcggcaactt gaccatcctc
     9841 tgccaacagt tcaccctcag gtgacctatg cctacatgaa aaacatgtgg aagagtgccc
     9901 gcaagatcga tgccttccag cacatgcagc attttgtcca gaccatgcag caacaggccc
     9961 agcatgccat cgctactgag gaccagcagc ataagcagga actgcacaag ctcatggccc
    10021 gatgcttcct gaaacttgga gagtggcagc tgaatctaca gggcatcaat gagagcacaa
    10081 tccccaaagt gctgcagtac tacagcgccg ccacagagca cgaccgcagc tggtacaagg
    10141 cctggcatgc gtgggcagtg atgaacttcg aagctgtgct acactacaaa catcagaacc
    10201 aagcccgcga tgagaagaag aaactgcgtc atgccagcgg ggccaacatc accaacgcca
    10261 ccactgccgc caccacggcc gccactgcca ccaccactgc cagcaccgag ggcagcaaca
    10321 gtgagagcga ggccgagagc accgagaaca gccccacccc atcgccgctg cagaagaagg
    10381 tcactgagga tctgtccaaa accctcctga tgtacacggt gcctgccgtc cagggcttct
    10441 tccgttccat ctccttgtca cgaggcaaca acctccagga tacactcaga gttctcacct
    10501 tatggtttga ttatggtcac tggccagatg tcaatgaggc cttagtggag ggggtgaaag
    10561 ccatccagat tgatacctgg ctacaggtta tacctcagct cattgcaaga attgatacgc
    10621 ccagaccctt ggtgggacgt ctcattcacc agcttctcac agacattggt cggtaccacc
    10681 cccaggccct catctaccca ctgacagtgg cttctaagtc taccacgaca gcccggcaca
    10741 atgcagccaa caagattctg aagaacatgt gtgagcacag caacaccctg gtccagcagg
    10801 ccatgatggt gagcgaggag ctgatccgag tggccatcct ctggcatgag atgtggcatg
    10861 aaggcctgga agaggcatct cgtttgtact ttggggaaag gaacgtgaaa ggcatgtttg
    10921 aggtgctgga gcccttgcat gctatgatgg aacggggccc ccagactctg aaggaaacat
    10981 cctttaatca ggcctatggt cgagatttaa tggaggccca agagtggtgc aggaagtaca
    11041 tgaaatcagg gaatgtcaag gacctcaccc aagcctggga cctctattat catgtgttcc
    11101 gacgaatctc aaagcagctg cctcagctca catccttaga gctgcaatat gtttccccaa
    11161 aacttctgat gtgccgggac cttgaattgg ctgtgccagg aacatatgac cccaaccagc
    11221 caatcattcg cattcagtcc atagcaccgt ctttgcaagt catcacatcc aagcagaggc
    11281 cccggaaatt gacacttatg ggcagcaacg gacatgagtt tgttttcctt ctaaaaggcc
    11341 atgaagatct gcgccaggat gagcgtgtga tgcagctctt cggcctggtt aacacccttc
    11401 tggccaatga cccaacatct cttcggaaaa acctcagcat ccagagatac gctgtcatcc
    11461 ctttatcgac caactcgggc ctcattggct gggttcccca ctgtgacaca ctgcacgccc
    11521 tcatccggga ctacagggag aagaagaaga tccttctcaa catcgagcat cgcatcatgt
    11581 tgcggatggc tccggactat gaccacttga ctctgatgca gaaggtggag gtgtttgagc
    11641 atgccgtcaa taatacagct ggggacgacc tggccaagct gctgtggctg aaaagcccca
    11701 gctccgaggt gtggtttgac cgaagaacca attatacccg ttctttagcg gtcatgtcaa
    11761 tggttgggta tattttaggc ctgggagata gacacccatc caacctgatg ctggaccgtc
    11821 tgagtgggaa gatcctgcac attgactttg gggactgctt tgaggttgct atgacccgag
    11881 agaagtttcc agagaagatt ccatttagac taacaagaat gttgaccaat gctatggagg
    11941 ttacaggcct ggatggcaac tacagaatca catgccacac agtgatggag gtgctgcgag
    12001 agcacaagga cagtgtcatg gccgtgctgg aagcctttgt ctatgacccc ttgctgaact
    12061 ggaggctgat ggacacaaat accaaaggca acaagcgatc ccgaacgagg acggattcct
    12121 actctgctgg ccagtcagtc gaaattttgg acggtgtgga acttggagag ccagcccata
    12181 agaaaacggg gaccacagtg ccagaatcta ttcattcttt cattggagac ggtttggtga
    12241 aaccagaggc cctaaataag aaagctatcc agattattaa cagggttcga gataagctca
    12301 ctggtcggga cttctctcat gatgacactt tggatgttcc aacgcaagtt gagctgctca
    12361 tcaaacaagc gacatcccat gaaaacctct gccagtgcta tattggctgg tgccctttct
    12421 ggtaactgga ggcccagatg tgcccatcac gttttttctg aggcttttgt actttagtaa
    12481 atgcttccac taaactgaaa ccatggtgag aaagtttgac tttgttaaat gggggatcca
    12541 ctagttctag agcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct
    12601 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
    12661 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    12721 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
    12781 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
    12841 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
    12901 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    12961 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    13021 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    13081 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
    13141 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    13201 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttga
//
