LOCUS       sequence-421943-        9843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of base editor dCas12a-A3G in mammalian cells for base 
            editing, along with non-fused EGFP fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, 
            Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao
            X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
  TITLE     Multiplexed base editing through Cas12a variant-mediated cytosine 
            and adenine base editors.
  JOURNAL   Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8.
  PUBMED    36323848
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-2"; volume: "5(1):1163. doi"; pages: " 
            10.1038/s42003-022-04152-8"
COMMENT     Sequence Label: pCMV-dCas12a-A3G
FEATURES             Location/Qualifiers
     source          1..9843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4765..4785
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4798..5046
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5059..5079
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5086..5139
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5140..5388
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5389..5409
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5410..5466
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5467..5715
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5716..5736
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5737..5790
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5791..6039
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6052..6072
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6082..6141
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             join(6142..6144,6145..6858)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6142..6144,6145..6855)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6184..6205)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6445..6464)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6792..6813
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6898..6915)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6904..7128
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7199..7215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7199..7215)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7212..7234)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7223..7239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7247..7253,7254..7271,7272..7277))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7292..7313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7430..7447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7601..8189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7681..7700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8360..9151,9152..9220))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8983..9002
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9221..9325)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9404..9423)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9595..9843,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgatcc ccccaccttc
      481 accttcaact tcaacaatga gccttgggtg aggggccgcc acgagaccta cctgtgctat
      541 gaggtggaga ggatgcacaa cgacacatgg gtgctgctga atcagcggag aggctttctg
      601 tgcaatcagg cccctcacaa gcacggcttc ctggagggcc ggcacgcaga gctgtgcttc
      661 ctggatgtga tcccattctg gaagctggac ctggatcagg actacagagt gacctgtttt
      721 acaagctggt ccccctgctt ctcttgtgcc caggagatgg ccaagtttat cagcaagaac
      781 aagcacgtgt ccctgtgcat cttcaccgcc cggatctacg acgatcaggg caggtgtcag
      841 gagggactgc gcacactggc agaggcagga gccaagatct ccatcatgac ctattctgag
      901 tttaagcact gctgggatac attcgtggac caccagggct gtccatttca gccctgggat
      961 ggcctggacg agcactctca ggacctgagc ggccggctga gagccatcct gcagaaccag
     1021 gagaatagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga aagtatgtca
     1081 aagctggaga aattcaccaa ctgttatagc ctgtctaaga ccctgcgctt caaggcaatc
     1141 ccagtgggca agacacaaga gaacattgac aacaaacggc tcctggtgga ggatgagaag
     1201 agggctgaag attacaaggg cgttaagaag ctgctggata ggtactatct gtcattcatc
     1261 aacgatgtcc tccacagtat caagctgaag aatctgaaca attacatttc tctgttccgg
     1321 aagaagacac ggaccgagaa ggagaacaaa gagctggaga atctggagat caacctgagg
     1381 aaagaaatag ctaaggcttt caaagggaac gagggttaca agtccctgtt caagaaagac
     1441 attatcgaga ctattctgcc tgagttcctg gacgataaag atgagatcgc cctcgtcaat
     1501 tccttcaatg ggtttaccac agcctttacc ggcttcttcg acaatagaga gaatatgttc
     1561 tctgaagagg ccaaatccac tagcatcgcc tttcgctgca taaacgagaa cctgactagg
     1621 tacatcagca atatggacat ctttgagaaa gtcgatgcca tattcgacaa acatgaggtg
     1681 caggagatta aggagaagat cctgaactca gattacgatg tcgaagattt cttcgaggga
     1741 gagttcttca acttcgtgct cacacaagag ggcattgatg tgtacaatgc aatcattgga
     1801 gggttcgtga cagagagtgg cgagaagata aagggcctga acgagtatat caacctctac
     1861 aaccagaaaa ccaagcagaa actgcctaag ttcaagccac tgtacaaaca agtgctctca
     1921 gatagggaaa gcctgagctt ctacggtgaa gggtatacat cagatgaaga agtgctcgaa
     1981 gtgttccgca acaccctcaa taagaacagt gaaatcttct cttcaatcaa gaagctggag
     2041 aaactgttca agaatttcga tgagtactcc tctgccggaa tctttgtgaa gaatggccct
     2101 gcaatatcca ctattagcaa agacatcttt ggcgagtgga acgttatcag ggataagtgg
     2161 aatgccgagt acgatgatat tcatctcaag aagaaagccg tggttacaga gaaatacgag
     2221 gatgatagac gcaagagctt taagaagatt ggtagcttct ctctcgaaca gctgcaggag
     2281 tacgccgacg ctgacctgtc agtcgtggag aaactcaagg agatcataat ccagaaggtg
     2341 gatgaaatct acaaagtgta tggaagctct gagaaactct tcgatgcaga ctttgttctg
     2401 gagaagagtc tgaagaagaa cgacgcagtg gttgctatca tgaaggacct gctggattct
     2461 gttaagtctt tcgagaatta cattaaggca ttctttggtg aagggaagga gacaaatagg
     2521 gacgagagct tctatggcga ctttgttctg gcctacgaca tcctcctcaa ggttgaccac
     2581 atctatgacg ctatacggaa ttacgttacc cagaagccct atagcaaaga caagttcaag
     2641 ctgtatttcc agaatccaca gtttatgggt gggtgggata aagacaaaga aacagattac
     2701 agggccacta tcctgcggta cggcagcaaa tactatctgg ctatcatgga taagaagtac
     2761 gccaaatgcc tccagaagat cgacaaggac gacgtgaacg gtaactacga gaagatcaat
     2821 tacaagctcc tgccaggacc taacaagatg ctgcccaagg tgttcttctc caagaaatgg
     2881 atggcctact ataacccaag cgaggacatt cagaagatat acaagaatgg gacattcaag
     2941 aagggcgata tgttcaacct caacgactgc cacaagctga ttgatttctt caaggatagc
     3001 atttctcgct atcccaagtg gtctaatgca tacgatttca acttcagcga gactgagaag
     3061 tacaaagaca tcgctggctt ctaccgggag gtggaagagc aaggctataa ggtgtcattc
     3121 gaatccgctt ctaagaagga agtggataag ctcgtggaag agggtaagct gtacatgttc
     3181 cagatataca acaaagactt cagcgataag agccacggca ctccaaacct ccatactatg
     3241 tatttcaagc tgctgtttga cgagaacaac cacggacaga ttaggctgtc aggaggcgca
     3301 gaactcttca tgcgcagagc ttcactgaag aaggaggaac tcgttgtcca cccagccaat
     3361 agccctatag ccaataagaa tccagacaat cctaagaaaa ccactactct gtcttacgat
     3421 gtgtataagg ataagagatt ctctgaagat cagtacgaac tgcacatacc cattgccatt
     3481 aacaagtgcc ctaagaacat cttcaagatt aacacagagg ttagagtgct cctgaaacac
     3541 gacgataacc cttatgttat aggcattgct cgcggagaga gaaacctgct gtacatcgtc
     3601 gtggtggacg gcaaaggcaa catcgtggaa cagtacagtc tcaatgaaat cattaacaat
     3661 ttcaacggaa tccgcattaa gaccgactac cattctctcc tcgacaagaa ggagaaagaa
     3721 aggttcgaag caagacagaa ttggacaagt atagagaata tcaaagaact gaaggctggg
     3781 tacatctctc aggttgtgca caagatatgt gagctggtgg agaagtacga cgctgttatc
     3841 gccctcgcgg acctgaatag cggcttcaag aactccaggg tgaaggtgga gaagcaggtg
     3901 tatcagaagt tcgagaagat gctgatcgac aagctcaact atatggtgga caagaaatcc
     3961 aatccttgcg ctactggtgg agccctgaag ggctatcaaa tcaccaataa gttcgaatct
     4021 ttcaagtcta tgagcaccca gaatggcttc atcttctaca tacccgcatg gctgacatcc
     4081 aagattgatc cctctaccgg atttgttaat ctgctcaaga ctaagtacac ctctattgct
     4141 gactcaaaga agttcatatc atcatttgac cgcatcatgt acgtgccaga agaggacctg
     4201 ttcgagtttg ccctggatta caagaatttc tctcggactg acgccgacta catcaagaag
     4261 tggaagctct actcttatgg taatcggatt cgcatattcc gcaatcccaa gaagaataac
     4321 gtgttcgatt gggaggaagt ttgcctcacc agcgcttaca aggagctgtt caataagtat
     4381 gggattaact accagcaggg cgacataaga gccctgctgt gcgaacaatc tgataaggca
     4441 ttctattcct ctttcatggc actgatgtca ctgatgctgc aaatgcgcaa ttccatcacc
     4501 ggaagaacag acgtggcctt tctgatctct cctgtcaaga actcagatgg catcttctac
     4561 gattcccgca actatgaagc acaggagaat gctatcctgc ctaagaatgc cgatgcaaat
     4621 ggagcctata acatcgccag aaaggtcctc tgggccatag gacaattcaa gaaagctgaa
     4681 gatgagaagc tggacaaggt gaagatcgcc atttcaaaca aagagtggct cgaatatgct
     4741 cagacctcag tgaagcatgg atcacccaag aagaaacgga aagtgtctgg tggttctact
     4801 aatctgtcag atattattga aaaggagacc ggtaagcaac tggttatcca ggaatccatc
     4861 ctcatgctcc cagaggaggt ggaagaagtc attgggaaca agccggaaag cgatatactc
     4921 gtgcacaccg cctacgacga gagcaccgac gagaatgtca tgcttctgac tagcgacgcc
     4981 cctgaataca agccttgggc tctggtcata caggatagca acggtgagaa caagattaag
     5041 atgctctctg gtggttctcc caagaagaag aggaaagtcg ggcccgaggg cagaggaagt
     5101 ctgctaacat gcggtgacgt cgaggagaat cctggcccaa ctaatctgtc agatattatt
     5161 gaaaaggaga ccggtaagca actggttatc caggaatcca tcctcatgct cccagaggag
     5221 gtggaagaag tcattgggaa caagccggaa agcgatatac tcgtgcacac cgcctacgac
     5281 gagagcaccg acgagaatgt catgcttctg actagcgacg cccctgaata caagccttgg
     5341 gctctggtca tacaggatag caacggtgag aacaagatta agatgctccc caagaagaag
     5401 aggaaagtcg ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct
     5461 ggacctacca acctgtccga tatcattgag aaagagaccg gcaaacagct ggtgatccag
     5521 gagagcatcc tgatgctgcc cgaagaggtg gaggaagtga tcggcaacaa gcccgagtcc
     5581 gacatcctgg tgcacacagc ctatgatgaa tccaccgacg agaacgtgat gctgctgacc
     5641 tccgatgctc ccgagtataa accctgggca ctggtgatcc aggactctaa tggagagaac
     5701 aagatcaaga tgctgcccaa gaagaagagg aaagtcgaag gacggggctc cctcctgacc
     5761 tgtggcgatg tggaagagaa ccccggcccc acaaacctca gtgacattat cgagaaggaa
     5821 acaggaaaac agctcgtcat tcaagaatct attcttatgt tgcctgagga agtcgaagag
     5881 gttattggca ataaacctga atctgatatt cttgtccata ccgcttacga tgagtccaca
     5941 gatgaaaatg ttatgctgct cacatctgac gcaccagagt acaaaccatg ggcgctcgtt
     6001 attcaagatt ccaacggcga aaacaaaatc aaaatgcttt ctggtggttc tcctaaaaaa
     6061 aaaagaaagg tgggctcggg ccagtgtact aattatgctc tcttgaaatt ggctggagat
     6121 gttgagagca acccaggtcc cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     6181 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     6241 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     6301 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     6361 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     6421 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     6481 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     6541 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     6601 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     6661 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     6721 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     6781 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     6841 gacgagctgt acaagtaaat catcaccatc accattgagt ttaaacccgc tgatcagcct
     6901 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     6961 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     7021 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     7081 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     7141 aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg taatcatggt
     7201 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7261 gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7321 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7381 gccaacgcgc gggaagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7441 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7501 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8041 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8221 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8281 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8341 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9361 gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc tgctctgatg
     9421 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     9481 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     9541 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     9601 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     9661 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     9721 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     9781 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     9841 atc
//
