LOCUS       sequence-421946-        9174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of base editor dCas12a-ABE7 in mammalian cells for base 
            editing, along with non-fused EGFP fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, 
            Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao
            X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
  TITLE     Multiplexed base editing through Cas12a variant-mediated cytosine 
            and adenine base editors.
  JOURNAL   Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8.
  PUBMED    36323848
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-2"; volume: "5(1):1163. doi"; pages: " 
            10.1038/s42003-022-04152-8"
COMMENT     Sequence Label: pCMV-dCas12a-ABE7
FEATURES             Location/Qualifiers
     source          1..9174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5383..5403
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5413..5472
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             join(5473..5475,5476..6189)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5473..5475,5476..6186)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5515..5536)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5776..5795)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6123..6144
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6229..6246)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6235..6459
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6530..6546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6530..6546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6543..6565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6554..6570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6578..6584,6585..6602,6603..6608))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6623..6644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6761..6778)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6932..7520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7012..7031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7691..8482,8483..8551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8314..8333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8552..8656)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8735..8754)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8926..9174,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga agtcgagttt
      481 agccacgagt attggatgag gcacgcactg accctggcaa agcgagcatg ggatgaaaga
      541 gaagtccccg tgggcgccgt gctggtgcac aacaatagag tgatcggaga gggatggaac
      601 aggccaatcg gccgccacga ccctaccgca cacgcagaga tcatggcact gaggcaggga
      661 ggcctggtca tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac actggagcca
      721 tgcgtgatgt gcgcaggagc aatgatccac agcaggatcg gaagagtggt gttcggagca
      781 cgggacgcca agaccggcgc agcaggctcc ctgatggatg tgctgcacca ccccggcatg
      841 aaccaccggg tggagatcac agagggaatc ctggcagacg agtgcgccgc cctgctgagc
      901 gatttcttta gaatgcggag acaggagatc aaggcccaga agaaggcaca gagctccacc
      961 gactctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac aagcgagtcc
     1021 gccacaccag agagctccgg cggctcctcc ggaggatcct ctgaggtgga gttttcccac
     1081 gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgcgatga gagggaggtg
     1141 cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc
     1201 atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg
     1261 gtcatgcaga actacagact gattgacgcc accctgtacg tgacattcga gccttgcgtg
     1321 atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac
     1381 gcaaaaaccg gcgccgcagg ctccctgatg gacgtgctgc actaccccgg catgaatcac
     1441 cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgctatttc
     1501 tttcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc caccgactcc
     1561 ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca
     1621 cctgaaagca gcgggggcag cagcgggggg tcatcaaagc tggagaaatt caccaactgt
     1681 tatagcctgt ctaagaccct gcgcttcaag gcaatcccag tgggcaagac acaagagaac
     1741 attgacaaca aacggctcct ggtggaggat gagaagaggg ctgaagatta caagggcgtt
     1801 aagaagctgc tggataggta ctatctgtca ttcatcaacg atgtcctcca cagtatcaag
     1861 ctgaagaatc tgaacaatta catttctctg ttccggaaga agacacggac cgagaaggag
     1921 aacaaagagc tggagaatct ggagatcaac ctgaggaaag aaatagctaa ggctttcaaa
     1981 gggaacgagg gttacaagtc cctgttcaag aaagacatta tcgagactat tctgcctgag
     2041 ttcctggacg ataaagatga gatcgccctc gtcaattcct tcaatgggtt taccacagcc
     2101 tttaccggct tcttcgacaa tagagagaat atgttctctg aagaggccaa atccactagc
     2161 atcgcctttc gctgcataaa cgagaacctg actaggtaca tcagcaatat ggacatcttt
     2221 gagaaagtcg atgccatatt cgacaaacat gaggtgcagg agattaagga gaagatcctg
     2281 aactcagatt acgatgtcga agatttcttc gagggagagt tcttcaactt cgtgctcaca
     2341 caagagggca ttgatgtgta caatgcaatc attggagggt tcgtgacaga gagtggcgag
     2401 aagataaagg gcctgaacga gtatatcaac ctctacaacc agaaaaccaa gcagaaactg
     2461 cctaagttca agccactgta caaacaagtg ctctcagata gggaaagcct gagcttctac
     2521 ggtgaagggt atacatcaga tgaagaagtg ctcgaagtgt tccgcaacac cctcaataag
     2581 aacagtgaaa tcttctcttc aatcaagaag ctggagaaac tgttcaagaa tttcgatgag
     2641 tactcctctg ccggaatctt tgtgaagaat ggccctgcaa tatccactat tagcaaagac
     2701 atctttggcg agtggaacgt tatcagggat aagtggaatg ccgagtacga tgatattcat
     2761 ctcaagaaga aagccgtggt tacagagaaa tacgaggatg atagacgcaa gagctttaag
     2821 aagattggta gcttctctct cgaacagctg caggagtacg ccgacgctga cctgtcagtc
     2881 gtggagaaac tcaaggagat cataatccag aaggtggatg aaatctacaa agtgtatgga
     2941 agctctgaga aactcttcga tgcagacttt gttctggaga agagtctgaa gaagaacgac
     3001 gcagtggttg ctatcatgaa ggacctgctg gattctgtta agtctttcga gaattacatt
     3061 aaggcattct ttggtgaagg gaaggagaca aatagggacg agagcttcta tggcgacttt
     3121 gttctggcct acgacatcct cctcaaggtt gaccacatct atgacgctat acggaattac
     3181 gttacccaga agccctatag caaagacaag ttcaagctgt atttccagaa tccacagttt
     3241 atgggtgggt gggataaaga caaagaaaca gattacaggg ccactatcct gcggtacggc
     3301 agcaaatact atctggctat catggataag aagtacgcca aatgcctcca gaagatcgac
     3361 aaggacgacg tgaacggtaa ctacgagaag atcaattaca agctcctgcc aggacctaac
     3421 aagatgctgc ccaaggtgtt cttctccaag aaatggatgg cctactataa cccaagcgag
     3481 gacattcaga agatatacaa gaatgggaca ttcaagaagg gcgatatgtt caacctcaac
     3541 gactgccaca agctgattga tttcttcaag gatagcattt ctcgctatcc caagtggtct
     3601 aatgcatacg atttcaactt cagcgagact gagaagtaca aagacatcgc tggcttctac
     3661 cgggaggtgg aagagcaagg ctataaggtg tcattcgaat ccgcttctaa gaaggaagtg
     3721 gataagctcg tggaagaggg taagctgtac atgttccaga tatacaacaa agacttcagc
     3781 gataagagcc acggcactcc aaacctccat actatgtatt tcaagctgct gtttgacgag
     3841 aacaaccacg gacagattag gctgtcagga ggcgcagaac tcttcatgcg cagagcttca
     3901 ctgaagaagg aggaactcgt tgtccaccca gccaatagcc ctatagccaa taagaatcca
     3961 gacaatccta agaaaaccac tactctgtct tacgatgtgt ataaggataa gagattctct
     4021 gaagatcagt acgaactgca catacccatt gccattaaca agtgccctaa gaacatcttc
     4081 aagattaaca cagaggttag agtgctcctg aaacacgacg ataaccctta tgttataggc
     4141 attgctcgcg gagagagaaa cctgctgtac atcgtcgtgg tggacggcaa aggcaacatc
     4201 gtggaacagt acagtctcaa tgaaatcatt aacaatttca acggaatccg cattaagacc
     4261 gactaccatt ctctcctcga caagaaggag aaagaaaggt tcgaagcaag acagaattgg
     4321 acaagtatag agaatatcaa agaactgaag gctgggtaca tctctcaggt tgtgcacaag
     4381 atatgtgagc tggtggagaa gtacgacgct gttatcgccc tcgcggacct gaatagcggc
     4441 ttcaagaact ccagggtgaa ggtggagaag caggtgtatc agaagttcga gaagatgctg
     4501 atcgacaagc tcaactatat ggtggacaag aaatccaatc cttgcgctac tggtggagcc
     4561 ctgaagggct atcaaatcac caataagttc gaatctttca agtctatgag cacccagaat
     4621 ggcttcatct tctacatacc cgcatggctg acatccaaga ttgatccctc taccggattt
     4681 gttaatctgc tcaagactaa gtacacctct attgctgact caaagaagtt catatcatca
     4741 tttgaccgca tcatgtacgt gccagaagag gacctgttcg agtttgccct ggattacaag
     4801 aatttctctc ggactgacgc cgactacatc aagaagtgga agctctactc ttatggtaat
     4861 cggattcgca tattccgcaa tcccaagaag aataacgtgt tcgattggga ggaagtttgc
     4921 ctcaccagcg cttacaagga gctgttcaat aagtatggga ttaactacca gcagggcgac
     4981 ataagagccc tgctgtgcga acaatctgat aaggcattct attcctcttt catggcactg
     5041 atgtcactga tgctgcaaat gcgcaattcc atcaccggaa gaacagacgt ggcctttctg
     5101 atctctcctg tcaagaactc agatggcatc ttctacgatt cccgcaacta tgaagcacag
     5161 gagaatgcta tcctgcctaa gaatgccgat gcaaatggag cctataacat cgccagaaag
     5221 gtcctctggg ccataggaca attcaagaaa gctgaagatg agaagctgga caaggtgaag
     5281 atcgccattt caaacaaaga gtggctcgaa tatgctcaga cctcagtgaa gcatggatca
     5341 tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa
     5401 gtcggctcgg gccagtgtac taattatgct ctcttgaaat tggctggaga tgttgagagc
     5461 aacccaggtc ccgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     5521 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     5581 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     5641 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     5701 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     5761 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     5821 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     5881 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     5941 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     6001 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     6061 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     6121 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     6181 tacaagtaaa tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
     6241 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6301 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6361 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6421 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     6481 gctggggctc gataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6541 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6601 agtgtaaagc ctagggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6661 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6721 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6781 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6841 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6901 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6961 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7021 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7081 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7141 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7201 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7261 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7321 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7381 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7441 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7501 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacactcagt
     7561 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7621 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7681 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7741 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7801 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7861 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7921 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7981 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8041 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8101 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8161 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8221 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8281 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8341 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8401 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8461 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8521 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8581 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8641 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     8701 atcgatctcc cgatccccta gggtcgactc tcagtacaat ctgctctgat gccgcatagt
     8761 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     8821 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     8881 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     8941 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     9001 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     9061 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     9121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatc
//
