LOCUS       sequence-422204-       11538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P
  TITLE     Nutrient control of glucose homeostasis through a complex of 
            PGC-1alpha and SIRT1.
  JOURNAL   Nature 2005 Mar 3;434(7029):113-8.
  PUBMED    15744310
REFERENCE   2  (bases 1 to 11538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2005-03-3"; volume: "434(7029)"; pages: "113-8"
COMMENT     Sequence Label: pAd-Track SIRT1
FEATURES             Location/Qualifiers
     source          1..11538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   79..181
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     enhancer        3440..3743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3744..3947
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4035..6248
                     /codon_start=1
                     /product="mouse NAD+-dependent protein deacetylase 
                     sirtuin-1"
                     /label=SIRT1
                     /note="homolog of budding yeast Sir2"
                     /translation="MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGR
                     SPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRR
                     EPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHA
                     SSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSE
                     PPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPD
                     LPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNID
                     TLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIM
                     KPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINR
                     EPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLS
                     ELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQT
                     SRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFV
                     PPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLED
                     DTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS"
     regulatory      4089..4098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6459..6580
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        6666..6969
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6970..7173
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      7212..7221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7218..7220,7221..7223,7224..7934)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7218..7220,7221..7223,7224..7934)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7263..7284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7524..7543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7871..7892
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8073..8194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_signal     8337..8487
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   8575..8677
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      8715..9095
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8802..8821)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9012..9033
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9143..9937)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(9143..9937)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9254..9273)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9864..9883
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      10769..11357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11258..11277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11511..11528
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatgga
       61 tccatgcatg ttaattaaca tcatcaataa tataccttat tttggattga agccaatatg
      121 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
      181 ggttttaggg cggagtaact tgtatgtgtt gggaattgta gttttcttaa aatgggaagt
      241 gacgtaacgt gggaaaacgg aagtgacgat ttgaggaagt tgtgggtttt ttggctttcg
      301 tttctgggcg taggttcgcg tgcggttttc tgggtgtttt ttgtggactt taaccgttac
      361 gtcatttttt agtcctatat atactcgctc tgcacttggc ccttttttac actgtgactg
      421 attgagctgg tgccgtgtcg agtggtgttt ttttaatagg ttttcttttt tactggtaag
      481 gctgactgtt atggctgccg ctgtggaagc gctgtatgtt gttctggagc gggagggtgc
      541 tattttgcct aggcaggagg gtttttcagg tgtttatgtg tttttctctc ctattaattt
      601 tgttatacct cctatggggg ctgtaatgtt gtctctacgc ctgcgggtat gtattccccc
      661 gggctatttc ggtcgctttt tagcactgac cgatgtgaat caacctgatg tgtttaccga
      721 gtcttacatt atgactccgg acatgaccga ggagctgtcg gtggtgcttt ttaatcacgg
      781 tgaccagttt ttttacggtc acgccggcat ggccgtagtc cgtcttatgc ttataagggt
      841 tgtttttcct gttgtaagac aggcttctaa tgtttaaatg tttttttgtt attttatttt
      901 gtgtttatgc agaaacccgc agacatgttt gagagaaaaa tggtgtcttt ttctgtggtg
      961 gttccggagc ttacctgcct ttatctgcat gagcatgact acgatgtgct ttcttttttg
     1021 cgcgaggctt tgcctgattt tttgagcagc accttgcatt ttatatcgcc gcccatgcaa
     1081 caagctattg aattcgttta aactccctct caagtctgta tacggggaca cggacagcct
     1141 tttcgtcacc gagcgtggac accggctcat ggaaaccaga ggtaagaaac gcatcaaaaa
     1201 gcatggggga aacctggttt ttgaccccga acggccagag ctcacctggc tcgtggaatg
     1261 cgagaccgtc tgcggggcct gcggcgcgga tgcctactcc ccggaatcgg tatttctcgc
     1321 gcccaagctc tacgccctca aaagtctgca ctgcccctcg tgcggcgcct cctccaaggg
     1381 caagctgcgc gccaagggcc acgccgcgga ggggctggac tatgacacca tggtcaaatg
     1441 ctacctggcc gacgcgcagg gcgaagaccg gcagcgcttc agcaccagca ggaccagcct
     1501 caagcgcacc ctggccagcg cgcagcccgg agcgcacccc ttcaccgtga cccagactac
     1561 gctgacgagg accctgcgcc cgtggaaaga catgaccctg gcccgtctgg acgagcaccg
     1621 actactgccg tacagcgaaa gccgccccaa cccgcgaaac gaggagatat gctggatcga
     1681 gatgccgtag agcaggtgac cgagctgtgg gaccgcctgg aactgcttgg tcaaacgctc
     1741 aaaagcatgc ctacggcgga cggtctcaaa ccgttgaaaa actttgcttc cttgcaagaa
     1801 ctgctatcgc tgggcggcga gcgccttctg gcggatttgg tcagggaaaa catgcgagtc
     1861 agggacatgc ttaacgaagt ggcccccctg ctcagggatg acggcagctg cagctctctt
     1921 aactaccagt tgcagccggt aataggtgtg atttacgggc ccaccggctg cggtaagtcg
     1981 cagctgctca ggaacctgct ttcttcccag ctgatctccc ctaccccgga aaccgttttc
     2041 ttcatcgccc cgcaggtaga catgatcccc ccatctgaac tcaaagcgtg ggaaatgcaa
     2101 atctgtgagg gtaactacgc ccctgggccg gatggaacca ttataccgca gtctggcacc
     2161 ctccgcccgc gctttgtaaa aatggcctat gacgatctca tcctggaaca caactatgac
     2221 gttagtgatc ccagaaatat cttcgcccag gccgccgccc gtgggcccat tgccatcatt
     2281 atggacgaat gcatggaaaa tcttggaggt cacaagggcg tctccaagtt cttccacgca
     2341 tttccttcta agctacatga caaatttccc aagtgcaccg gatacactgt gctggtggtt
     2401 ctgcacaaca tgaatccccg gagggatatg gctgggaaca tagccaacct aaaaatacag
     2461 tccaagatgc atctcatatc cccacgtatg cacccatccc agcttaaccg ctttgtaaac
     2521 acttacacca agggcctgcc cctggcaatc agcttgctac tgaaagacat ttttaggcac
     2581 cacgcccagc gctcctgcta cgactggatc atctacaaca ccaccccgca gcatgaagct
     2641 ctgcagtggt gctacctcca ccccagagac gggcttatgc ccatgtatct gaacatccag
     2701 agtcaccttt accacgtcct ggaaaaaata cacaggaccc tcaacgaccg agaccgctgg
     2761 tcccgggcct accgcgcgcg caaaacccct aaataaagac agcaagacac ttgcttgatc
     2821 caaatccaaa cagagtctgg ttttttattt atgttttaaa ccgcattggg aggggaggaa
     2881 gccttcaggg cagaaacctg ctggcgcaga tccaacagct gctgagaaac gacattaagt
     2941 tcccgggtca aagaatccaa ttgtgccaaa agagccgtca acttgtcatc gcgggcggat
     3001 gaacgggaag ctgcactgct tgcaagcggg ctcaggaaag caaagtcagt cacaatcccg
     3061 cgggcggtgg ctgcagcggc tgaagcggcg gcggaggctg cagtctccaa cggcgttcca
     3121 gacacggtct cgtaggtcaa ggtagtagag tttgcgggca ggacggggcg accatcaatg
     3181 ctggagccca tcacattctg acgcaccccg gcccatgggg gcatgcgcgt tgtcaaatat
     3241 gagctcacaa tgcttccatc aaacgagttg gtgctcatgg cggcggcggc tgctgcaaaa
     3301 cagatacaaa actacataag acccccacct tatatattct ttcccaccct taaccacgcc
     3361 cagatcctct agcagtgata aacgtctaat agtaatcaat tacggggtca ttagttcata
     3421 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3481 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3541 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3601 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3661 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3721 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3781 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     3841 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3901 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     3961 gtcagatccg ctagagatct ggtaccgagc tcggatccga attcgagctc cgtcgacaga
     4021 gaggcagttg gaagatggcg gacgaggtgg cgctcgccct tcaggccgcc ggctcccctt
     4081 ccgcggcggc cgccatggag gccgcgtcgc agccggcgga cgagccgctc cgcaagaggc
     4141 cccgccgaga cgggcctggc ctcgggcgca gcccgggcga gccgagcgca gcagtggcgc
     4201 cggcggccgc ggggtgtgag gcggcgagcg ccgcggcccc ggcggcgctg tggcgggagg
     4261 cggcaggggc ggcggcgagc gcggagcggg aggccccggc gacggccgtg gccggggacg
     4321 gagacaatgg gtccggcctg cggcgggagc cgagggcggc cgacgacttc gacgacgacg
     4381 agggcgagga ggaggacgag gcggcggcgg cagcggcggc ggcagcgatc ggctaccgag
     4441 acaacctcct gttgaccgat ggactcctca ctaatggctt tcattcctgt gaaagtgatg
     4501 acgatgacag aacgtcacac gccagctcta gtgactggac tccgcggccg cggataggtc
     4561 catatacttt tgttcagcaa catctcatga ttggcaccga tcctcgaaca attcttaaag
     4621 atttattacc agaaacaatt cctccacctg agctggatga tatgacgctg tggcagattg
     4681 ttattaatat cctttcagaa ccaccaaagc ggaaaaaaag aaaagatatc aatacaattg
     4741 aagatgctgt gaagttactg caggagtgta aaaagataat agttctgact ggagctgggg
     4801 tttctgtctc ctgtgggatt cctgacttca gatcaagaga cggtatctat gctcgccttg
     4861 cggtggactt cccagacctc ccagaccctc aagccatgtt tgatattgag tattttagaa
     4921 aagacccaag accattcttc aagtttgcaa aggaaatata tcccggacag ttccagccgt
     4981 ctctgtgtca caaattcata gctttgtcag ataaggaagg aaaactactt cgaaattata
     5041 ctcaaaatat agataccttg gagcaggttg caggaatcca aaggatcctt cagtgtcatg
     5101 gttcctttgc aacagcatct tgcctgattt gtaagtacaa agttgattgt gaagctgttc
     5161 gtggagacat ttttaatcag gtagttcctc ggtgccctag gtgcccagct gatgagccac
     5221 ttgccatcat gaagccagag attgtcttct ttggtgaaaa cttaccagaa cagtttcata
     5281 gagccatgaa gtatgacaaa gatgaagttg acctcctcat tgttattgga tcttctctga
     5341 aagtgagacc agtagcacta attccaagtt ctatacccca tgaagtgcct caaatattaa
     5401 taaataggga acctttgcct catctacatt ttgatgtaga gctccttgga gactgcgatg
     5461 ttataattaa tgagttgtgt cataggctag gtggtgaata tgccaaactt tgttgtaacc
     5521 ctgtaaagct ttcagaaatt actgaaaaac ctccacgccc acaaaaggaa ttggttcatt
     5581 tatcagagtt gccaccaaca cctcttcata tttcggaaga ctcaagttca cctgaaagaa
     5641 ctgtaccaca agactcttct gtgattgcta cacttgtaga ccaagcaaca aacaacaatg
     5701 ttaatgattt agaagtatct gaatcaagtt gtgtggaaga aaaaccacaa gaagtacaga
     5761 ctagtaggaa tgttgagaac attaatgtgg aaaatccaga ttttaaggct gttggttcca
     5821 gtactgcaga caaaaatgaa agaacttcag ttgcagaaac agtgagaaaa tgctggccta
     5881 atagacttgc aaaggagcag attagtaagc ggcttgaggg taatcaatac ctgtttgtac
     5941 caccaaatcg ttacatattc cacggtgctg aggtatactc agactctgaa gatgacgtct
     6001 tgtcctctag ttcctgtggc agtaacagtg acagtggcac atgccagagt ccaagtttag
     6061 aagaaccctt ggaagatgaa agtgaaattg aagaattcta caatggcttg gaagatgata
     6121 cggagaggcc cgaatgtgct ggaggatctg gatttggagc tgatggaggg gatcaagagg
     6181 ttgttaatga agctatagct acaagacagg aattgacaga tgtaaactat ccatcagaca
     6241 aatcataaca ctattgaagc tgtccggatt caggaattgg tcgacaagct tgcggccgca
     6301 ctcgagccta agcttctaga taagatatcc gatccaccgg atctagataa ctgatcataa
     6361 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     6421 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     6481 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6541 attctagttg tggtttgtcc aaactcatca atgtatctta acgcggaggt ttatcgacga
     6601 tctgctagtg attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     6661 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     6721 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     6781 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     6841 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     6901 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     6961 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     7021 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     7081 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     7141 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
     7201 cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     7261 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     7321 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     7381 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     7441 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     7501 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     7561 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     7621 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     7681 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     7741 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     7801 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     7861 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     7921 acgagctgta caagtccgga ctcagatcca ccggatctag ataactgatc ataatcagcc
     7981 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     8041 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     8101 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8161 gttgtggttt gtccaaactc atcaatgtat cttaacgcga tcaagctagc ttgctagact
     8221 cgactgacta taataataaa acgccaactt tgacccggaa cgcggaaaac acctgagaaa
     8281 aacacctggg cgagtctcca cgtaaacggt caaagtcccc gcggccctag acaaatatta
     8341 cgcgctatga gtaacacaaa attattcaga tttcacttcc tcttattcag ttttcccgcg
     8401 aaaatggcca aatcttactc ggttacgccc aaatttacta caacatccgc ctaaaaccgc
     8461 gcgaaaattg tcacttcctg tgtacaccgg cgcacaccaa aaacgtcact tttgccacat
     8521 ccgtcgctta catgtgttcc gccacacttg caacatcaca cttccgccac actactacgt
     8581 cacccgcccc gttcccacgc cccgcgccac gtcacaaact ccaccccctc attatcatat
     8641 tggcttcaat ccaaaataag gtatattatt gatgatgtta attaagaatt aattcgatcc
     8701 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8761 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8821 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8881 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8941 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     9001 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     9061 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     9121 ttaacaaaaa ttttaacaaa attcagaaga actcgtcaag aaggcgatag aaggcgatgc
     9181 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     9241 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     9301 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     9361 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc
     9421 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     9481 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     9541 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     9601 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     9661 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     9721 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     9781 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     9841 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     9901 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     9961 cttgatcaga gcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt
    10021 ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc
    10081 ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct
    10141 gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc
    10201 ggggtcagca ccgtttctgc ggactggctt tctacgtgaa aaggatctag gtgaagatcc
    10261 tttttgataa tctcatggct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10441 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    10921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    11161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    11221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    11281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    11341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    11521 tgagcgagga agcggaag
//
