LOCUS       sequence-422243-        8892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express split-RhoA fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C
  TITLE     Standardized Parts for Activation of Small GTPase Signaling in 
            Living Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 
            10.1002/anie.202403499.
  PUBMED    39058298
REFERENCE   2  (bases 1 to 8892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499."
COMMENT     Sequence Label: 
            pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..8892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      144..279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             344..1138
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(398..417)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1008..1027
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1202..1250
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(1291..1310)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1410..1432
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1470..1488)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1556..1660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1661..1729,1730..2521)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1879..1898)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2692..3280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3181..3200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3491..3870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3871..4082
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4033..4053
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4079..4103
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4243..4375
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4358..4377
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4420..4438
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4444..4446,4447..4449,4450..5160)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5185..5454
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     misc_feature    join(5607..6181,6182..6184,6185..6193)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5774..5791)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6001..6020
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6799..7119
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(7144..7146,7147..7857)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    7949..8070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7960..7979)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8014..8033
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      8256..8711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8343..8362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8553..8574
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(8828..8892,1..293)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(8828..8892,1..127)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8851..8871)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
       61 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      121 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      181 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      241 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
      301 attcttctga cacaacagtc tcgaacttaa ggctagagcc accatgattg aacaagatgg
      361 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
      421 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
      481 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
      541 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
      601 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
      661 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
      721 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
      781 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
      841 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
      901 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
      961 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     1021 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     1081 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     1141 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg
     1201 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc
     1261 gataaggatc cgcgtatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     1321 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     1381 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     1441 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1501 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1621 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1681 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1741 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1801 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1861 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     1921 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1981 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2041 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2101 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2161 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2221 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2281 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2341 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2401 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2461 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2521 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2581 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2641 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2701 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2761 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2821 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2881 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2941 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3001 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3061 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3121 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3181 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3241 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3301 tttacggttc ctggcctttt gctggccttt tgctcacatg gctcgacaga tcttcaatat
     3361 tggccattag ccatattatt cattggttat atagcataaa tcaatattgg ctattggcca
     3421 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
     3481 ccgccatgtt ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     3541 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     3601 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     3661 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     3721 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
     3781 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
     3841 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
     3901 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     3961 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctgcgatcgc
     4021 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     4081 ctcgtttagt gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa
     4141 ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agtgactctc
     4201 ttaaggtagc cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
     4261 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
     4321 ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc
     4381 cactcccagt tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct
     4441 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     4501 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     4561 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     4621 accctcgtga ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg
     4681 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     4741 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     4801 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     4861 cacaagctgg agtacaacgc catcagcgac aacgtctata tcaccgccga caagcagaag
     4921 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     4981 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5041 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     5101 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     5161 ggcggtggct catctggcgg aggtgagatg tggcatgaag gcctggaaga ggcatctcgt
     5221 ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct
     5281 atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc ctatggtcga
     5341 gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa tgtcaaggac
     5401 ctcacccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa gcagcagatc
     5461 tcgtacgcgt cccggggcgg tggctcatct ggcggaggtg agctcgctgc catccggaag
     5521 aaactggtga ttgttggtga tggagcctaa gaattcacgc gtcgagcatg catctagggc
     5581 ggccaattcc gcccctctcc cccccccccc tctccctccc ccccccctaa cgttactggc
     5641 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     5701 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     5761 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     5821 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     5881 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     5941 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     6001 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6061 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     6121 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     6181 gcttgccaca acccgggatc ctctagaatg tgtggaaaga actgcttgct catagtcttc
     6241 agcaaggacc agttcccaga ggtgtatgtg cccacagtgt ttgagaacta tgtggcagat
     6301 atcgaggtgg atggaaagca ggtagagttg gctttgtggg acacagctgg gcaggaagat
     6361 tatgatcgcc tgaggcccct ctcctaccca gataccgatg ttatactgat gtgtttttcc
     6421 atcgacagcc ctgatagttt agaaaacatc ccagaaaagt ggaccccaga agtcaagcat
     6481 ttctgtccca acgtgcccat catcctggtt gggaataaga aggatcttcg gaatgatgag
     6541 cacacaaggc gggagctagc caagatgaag caggagccgg tgaaacctga agaaggcaga
     6601 gatatggcaa acaggattgg cgcttttggg tacatggagt gttcagcaaa gaccaaagat
     6661 ggagtgagag aggtttttga aatggctacg agagctgctc tgcaagctgg ggatcccaat
     6721 tgggagctcg tgtacacggc gcgcctgcag ggaggtggct catctggcgg aggtcagatc
     6781 tcgtacgcgt cccggggcgg agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc
     6841 ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag
     6901 aaatttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag
     6961 gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg
     7021 actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat
     7081 gccactctcg tcttcgatgt ggagcttcta aaactggaag gcggtggctc atctggcgga
     7141 ggtgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     7201 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     7261 ggcaagctga ccctgaagct catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     7321 ctcgtgacca ccctcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     7381 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     7441 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     7501 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     7561 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
     7621 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     7681 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     7741 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     7801 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagaaa
     7861 aaaaagaaga aaaagagcaa gaccaaatgc gtgattatgt aagcggccgc ttccctttag
     7921 tgagggttaa tgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     7981 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     8041 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     8101 caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     8161 aaaatccgat aaggatcgat ccgggctggc gtaatagcga agaggcccgc accgatcgcc
     8221 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
     8281 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8341 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8401 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8461 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     8521 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     8581 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     8641 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     8701 gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     8761 atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag
     8821 gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8881 ccccaggctc cc
//
