LOCUS       sequence-422354-        8845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human eGFP-SUN1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Shokrollahi M, Stanic M, Hundal A, Chan JNY, Urman D, Hakem A, 
            Garcia RE, Hao J, Maass PG, Dickson BC, Hande MP, Pujana MA, Hakem 
            R, Mekhail K
  TITLE     DNA double-strand break-capturing nuclear envelope tubules drive DNA
            repair
  JOURNAL   bioRxiv 2023.05.07.539750
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.07.539750"
COMMENT     Sequence Label: eGFP-SUN1
FEATURES             Location/Qualifiers
     source          1..8845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             964..984
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1000..1002,1003..1005,1006..1716)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1000..1002,1003..1005,1006..1716)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1045..1066)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1306..1325)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1653..1674
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4436..4453)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4442..4666
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4712..5140
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4799..4818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5009..5030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5149..5169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5154..5483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5334..5469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5396..5415
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5550..6344
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5604..5623)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6214..6233
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6518..6639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6555..6574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6609..6628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6688..6704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6688..6704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6701..6723)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6712..6728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6736..6742,6743..6760,6761..6766))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6781..6802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6919..6936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7090..7678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7170..7189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7849..8640,8641..8709))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8472..8491
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8710..8814)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgc ttaagggagg tggatctgga ggtggatctg gaggtggatc tggaggtgga
      961 tctcccaaga agaagaggaa ggtgggaggt tctaccggta tggtgagcaa gggcgaggag
     1021 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1081 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1141 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1201 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1261 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1321 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1381 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1441 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1501 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1561 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1621 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1681 gccgggatca ctctcggcat ggacgagctg tacaagggag gttctgttaa catggatttt
     1741 tctcggcttc acatgtacag tcctccccag tgtgtgccgg agaacacggg ctacacgtat
     1801 gcgctcagtt ccagctattc ttcagatgct ctggattttg agacggagca caaattggac
     1861 cctgtatttg attctccacg gatgtcccgc cgtagtttgc gcctggccac gacagcatgc
     1921 accctggggg atggtgaggc tgtgggtgcc gacagcggca ccagcagcgc tgtctccctg
     1981 aagaaccgag cggccagaac aacaaaacag cgcagaagca caaacaaatc agcttttagt
     2041 atcaaccacg tgtcaaggca ggtcacgtcc tctggcgtca gccacggcgg cactgtcagc
     2101 ctgcaggatg ctgtgactcg acggcctcct gtattggacg agtcttggat tcgtgaacag
     2161 accacagtgg accacttctg gggtcttgat gatgatggtg atcttaaagg tggaaataaa
     2221 gctgccattc agggaaacgg ggatgtggga gccgccgccg ccaccgcgca caacggcttc
     2281 tcctgcagca actgcagcat gctgtccgag cgcaaggacg tgctcacggc gcaccccgcg
     2341 gcccccgggc ccgtgtcgag agtttattct agggacagga atcaaaaatg cggtgcgtct
     2401 ttctacgtga ataggatttt gtggctggcc agatacactg catcatcttt ttcatcattt
     2461 ttagttcaac tttttcaagt ggttttaatg aagctcagtt atgaatcaga aaattacaaa
     2521 ttgaaaactc atgaatcaaa agattgtgaa tccgaaagct ataagtcaaa aagccatgaa
     2581 tcaaaaggcg acgactgtaa gggcaagagg cacctcgacg cgcacacagc cgcccactcg
     2641 cagtcgccac ggctgcccgg tcgggcaggg accctctggc acatctgggc atgtgcaggt
     2701 tacttcttgc tgcagattct gcgcaggatc ggagctgtgg gccaggctgt gtccaggacg
     2761 gcgtggtcgg ccctttggct ggccgtggtt gctccaggga aggcagcctc tggagtgttc
     2821 tggtggctgg ggattggatg gtaccagttt gttactttga tttcttggct gaatgtgttt
     2881 cttcttacca ggtgccttcg aaacatctgc aagtttttag tcttgctcat cccactcttc
     2941 cttttactag caggtctctc cttacggggc cagggcaatt tcttttcgtt cttgcccgtg
     3001 ttgaattggg caagcatgca tagaacacag cgggtggatg acccccagga cgtgtttaaa
     3061 cccacgactt ctcgcctgaa gcagcctctg cagggtgaca gtgaggcttt tccgtggcat
     3121 tggatgagtg gcgtggagca gcaggtggcc tctctgtctg gacagtgcca ccaccatggt
     3181 gagaatctcc gagagctgac cactttgcta cagaagctgc aggctcgggt ggaccagatg
     3241 gaaggcggcg ctgccgggcc gtcagcttcg gtcagagacg ctgtgggaca gcccccgagg
     3301 gagactgact ttatggcctt tcaccaagaa catgaagtgc gtatgtcaca cttggaagat
     3361 attctgggaa aactgagaga aaaatctgag gccatccaga aggaactaga acagaccaag
     3421 caaaaaacaa tcagtgcggt tggtgagcag ctcctgccca cggtcgagca cctccagctg
     3481 gagctggatc agctaaagtc agagctgtcc agctggcgac acgtgaagac cggctgtgag
     3541 acagtggatg ccgtacaaga aagagtggac gtgcaagtca gagaaatggt gaaactcctg
     3601 ttttccgaag atcagcaagg cggttctctg gaacagctgc tgcagaggtt ctcatcacag
     3661 tttgtgagca aaggcgactt gcagacgatg ctgcgagacc tgcagctgca gatcctgcgg
     3721 aacgtcaccc accacgtttc cgtgaccaag cagctcccaa cctcagaagc cgtggtgtct
     3781 gctgtgagcg aggcgggggc gtctggaata acagaggcgc aagcacgtgc catcgtgaac
     3841 agcgccttga agctgtattc ccaagataag accgggatgg tggactttgc tctggaatct
     3901 ggtggtggca gcatcttgag tactcgctgt tctgaaactt acgaaaccaa aacggcgctg
     3961 atgagtctgt ttgggatccc gctgtggtac ttctcgcagt ccccgcgcgt ggtcatccag
     4021 cctgacattt accccggtaa ctgctgggca tttaaaggct cccaggggta cctggtggtg
     4081 aggctctcca tgatgatcca cccagccgcc ttcactctgg agcacatccc taagacgctg
     4141 tcgccaacag gcaacatcag cagcgccccc aaggacttcg ccgtctatgg attagaaaat
     4201 gagtatcagg aagaagggca gcttctggga cagttcacgt atgatcagga tggggagtcg
     4261 ctccagatgt tccaggccct gaaaagaccc gacgacacag ctttccaaat agtggaactt
     4321 cggatttttt ctaactgggg ccatcctgag tatacctgtc tgtatcggtt cagagttcat
     4381 ggcgaacctg tcaagtgact cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     4441 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4501 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4561 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4621 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4681 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4741 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4801 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4861 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4921 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4981 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5041 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5101 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5161 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5221 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5281 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5341 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5401 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5461 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5521 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5581 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5641 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5701 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5761 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5821 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5881 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5941 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6001 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6061 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6121 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6181 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6241 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6301 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6361 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     6421 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6481 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6541 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6601 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6661 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6721 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6781 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6841 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6901 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7261 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7501 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7861 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7921 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7981 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8041 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8101 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8161 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8221 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8281 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8341 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8401 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8461 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8521 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8581 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8641 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8701 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8761 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8821 tccccgaaaa gtgccacctg acgtc
//
