LOCUS       sequence-422548-        2368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with ampicillin resistance for intermediate 
            expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm1.3
FEATURES             Location/Qualifiers
     source          1..2368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..169
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     120..140
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    344..568
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(638..655)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(638..654)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        654..725
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(726..794,795..1586)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(944..963)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     1587..1606
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1587..1605
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1694..1712)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      1735..2323
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2224..2243
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2342..2358
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2342..2358
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 taagtgacta gcggaaacac agaaaaaagc ccgcacctga cagtgcgagc tttttttttc
       61 gaccaaaggc caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      121 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccgaagagc
      181 cgagtgtctt cggttcgaga ccgaattcgc gagctctcgc tagccagttt aaacaattgc
      241 ggccgcgtac tagtctatcg atttggatcc accctgcagg aagtctagac cgtcgacacg
      301 gtctctgaga gaagacacgt cgctcttcga cgtaagtaac tgactgtgcc ttctagttgc
      361 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
      421 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
      481 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
      541 catgctgggg atgcggtggg ctctatggct cggtaccaaa ttccagaaaa gaggcctccc
      601 gaaagggggg ccttttttcg ttttggtcct aagcttaact ggccgtcgtt ttacattcaa
      661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      901 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      961 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1021 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1081 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1141 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1201 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1261 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1321 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1381 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1441 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1501 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1561 gtgcctcact gattaagcat tggtaataat acgactcact atagggtaac taattgaggc
     1621 gcgcctacca ccgtcaaaaa aaacggcgct ttttagcgcc gtttttattt ttcaaccttc
     1681 ctaggtaagt gaccctttag tgagggttaa tttagaaaag atcaaaggat cttcttgaga
     1741 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1801 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1861 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1921 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1981 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2041 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2101 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2161 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2221 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2281 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2341 ccaggaaaca gctatgacca tgcccggg
//
