LOCUS       sequence-422553-        2686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with ampicillin resistance for high expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm2.2
FEATURES             Location/Qualifiers
     source          1..2686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    749..883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(786..805)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     840..859
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(953..970)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(953..969)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        969..1040
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1041..1109,1110..1901)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1259..1278)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     1902..1921
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1902..1920
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2009..2027)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      2050..2638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2539..2558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2657..2673
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2657..2673
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtgactagcg gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac
       61 caaaggcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctctccga agagccgagt gtcttcggtt
      601 cgagaccgaa ttcgcgagct ctcgctagcc agtttaaaca attgcggccg cgtactagtc
      661 tatcgatttg gatccaccct gcaggaagtc tagaccgtcg acacggtctc tgagagaaga
      721 cacgtcgctc ttcgacgtaa gtaactgaaa cttgtttatt gcagcttata atggttacaa
      781 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
      841 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcctcggta ccaaattcca
      901 gaaaagaggc ctcccgaaag gggggccttt tttcgttttg gtcctaagct taactggccg
      961 tcgttttaca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1021 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1081 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1141 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1201 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1261 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     1321 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1381 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1441 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1501 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1561 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1621 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1681 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1741 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1801 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1861 agatcgctga gataggtgcc tcactgatta agcattggta ataatacgac tcactatagg
     1921 gtaactaatt gaggcgcgcc taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt
     1981 tatttttcaa ccttcctagg taagtgaccc tttagtgagg gttaatttag aaaagatcaa
     2041 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2101 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2161 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2221 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2281 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2341 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2401 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2461 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2521 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2581 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2641 cgccagcaac gcggcccagg aaacagctat gaccatgccc gggtaa
//
