LOCUS       sequence-422689-       11437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian-cell-based plasmid system that expresses mAIRN (an 
            R-loop-forming transcript) upon dox-induction, which will further 
            cause co-directional transcription-replication conflict (CD TRC)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Hamperl S, Bocek MJ, Saldivar JC, Swigut T, Cimprich KA
  TITLE     Transcription-Replication Conflict Orientation Modulates R-Loop 
            Levels and Activates Distinct DNA Damage Responses.
  JOURNAL   Cell. 2017 Aug 10;170(4):774-786.e19. doi: 
            10.1016/j.cell.2017.07.043.
  PUBMED    28802045
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "774-786.e19"
COMMENT     Sequence Label: mAIRN CD
FEATURES             Location/Qualifiers
     source          1..11437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      489..2278
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2580..4505)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(4940..4958)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5026..5130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5131..5199,5200..5991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5349..5368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6162..6750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6651..6670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7306..7451
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7494..8531
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(8529..8548)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8573..8621
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     9135..9157
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    9300..9434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9337..9356)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9391..9410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9441..9460
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9441..9459
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(10826..11140)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(10829..10853)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    10900..10918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10936..10954
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10971..10989
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11007..11025
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11043..11061
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11078..11096
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11114..11132
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tgataagctg tcaaacatga gaattggtcg actctagagg atcgatcccc gccccggacg
       61 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
      121 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
      181 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
      241 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
      301 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
      361 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
      421 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
      481 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
      541 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
      601 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
      661 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
      721 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
      781 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
      841 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
      901 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
      961 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     1021 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     1081 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     1141 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     1201 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     1261 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     1321 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     1381 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     1441 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     1501 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     1561 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     1621 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     1681 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     1741 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1801 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     1861 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     1921 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     1981 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     2041 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     2101 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     2161 tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct
     2221 ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct
     2281 ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat
     2341 tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct
     2401 gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga
     2461 caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct
     2521 ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat
     2581 cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca
     2641 tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca
     2701 tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca
     2761 aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg
     2821 aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt
     2881 ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct
     2941 cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa
     3001 gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct
     3061 gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct
     3121 ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct
     3181 gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg
     3241 ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt
     3301 ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc
     3361 ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc
     3421 ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca
     3481 ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct
     3541 gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc
     3601 cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
     3661 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
     3721 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     3781 gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct
     3841 cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgctcct
     3901 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     3961 cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc
     4021 cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     4081 gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct
     4141 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
     4201 cctcctgctc ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg
     4261 cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg tcttggccct
     4321 gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt
     4381 cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt
     4441 ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca
     4501 gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata
     4561 cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc
     4621 gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc
     4681 accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc
     4741 ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc
     4801 tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga
     4861 agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag
     4921 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4981 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5041 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5101 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5341 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     5401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5641 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     5701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6301 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6781 ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc
     6841 atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag
     6901 gccatccagc ctcgcgtcgg atctcatggt gctgtatata aaaccagtgg ttatatgtac
     6961 agtagctgta tataaaacca gtggttatat gtacagtagc tgtatataaa accagtggtt
     7021 atatgtacag tagtacgtta gatccgaacg ccagcaagac gtagcccagc gcgtcggccc
     7081 cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg
     7141 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
     7201 gaatccgtta gcgaggtgcc gccctgcttc atccccgtgg cccgttgctc gcgtttgctg
     7261 gcggtgtccc cggaagaaat atatttgcat gtctttagtt ctatgatgac acaaaccccg
     7321 cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg gcgcggtccg
     7381 aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg accctgcagc
     7441 gacccgctta acagcgtcaa cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa
     7501 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     7561 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     7621 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     7681 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     7741 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     7801 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     7861 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     7921 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     7981 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     8041 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     8101 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     8161 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     8221 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     8281 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     8341 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     8401 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     8461 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccggatcgg gagatggggg
     8521 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     8581 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     8641 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     8701 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     8761 cgtcggggcg gcaggccctg ccatagccac tggccccgtg ggttagggac ggggtccccc
     8821 atggggaatg gtttatggtt cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc
     8881 cacgactgga ctgagcagac agacccatgg tttttggatg gcctgggcat ggaccgcatg
     8941 tactggcgcg acacgaacac cgggcgtctg tggctgccaa acacccccga cccccaaaaa
     9001 ccaccgcgcg gatttctggc gtgccaagct agtcgacctc atggctgcgc cccgacaccc
     9061 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     9121 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     9181 cgcgaggcag ccggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     9241 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     9301 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9361 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9421 atcatgtctg gatcctgcag taatacgact cactataggg cgaattggag ctccacatca
     9481 agcttgagct tgcctctctt gcaacgtggc acttttgagt tcatctctcc tgtaacatgg
     9541 cacttttgag cgttggccct gatcacagaa cccttcgaat cctccccttg tgcagtttgc
     9601 accctcagga tacctcggaa tcctccgagc cgtctcttcc cctcccctcg cgcaacttgg
     9661 cataaccaga atcacagccc aaccggaatc gcattaaaac cctccgaacc tttgggcagc
     9721 gcggcaccgg ggctcacgca gtgccgcgga accctcggaa ccctgccctt gtgcagcttc
     9781 gcaccctcag gatagctcgg aaccctctga gctttccctt ccctttccct caccgcaact
     9841 cagcacaacc aaggcacatg gtcctgctgg agttcgccaa ggatcacggc acaaccggaa
     9901 ttgctccgaa ccctcgggca gcactgcggc gcagtgccgc ggaaccctct aaaccctccg
     9961 aatcccctcc ttgtgcagct tttcaccctt agcctctctc gcaactcggc acaaccagaa
    10021 tcgcagcaca attggagttg tgctaaaatc ctctgaacgg gggggggggg gggggggcag
    10081 cgcacacgcc agcaccctgg ctcgcgcagt gccgcggaac ccaccgaacc ttccccctgc
    10141 cccgcaaacc gcggatcagg tcgtgtagaa atcctccgat ctccaccccc cccccaactc
    10201 tccagcagcg tggtgcccta gcgcgaaccc ctgctcccct tgcgcagcgt ggcaccctcg
    10261 tgctctagat cgcccgtaaa cccccccccc ccccagtgca gcagtcggcc cgcgctggac
    10321 gcccccctcc cttctcctct tgctgacgcg gcatggcggc agcgcggctt tcggctttct
    10381 accggcccta tcggccctcg tgtagttcag aacactggtg agcagtgggg caccctagtg
    10441 ctgagttttc ttgtagccca gaaatcttca ccctagcgct gaatctcgcg taggggaacc
    10501 tttgagcggc ctggaactct tggtcggagc cctcgagctg ccggatcagt ggaaccctca
    10561 cctcgcgtag agcccttccc tcctgtggaa cccttccttt gcggaatcct ctaacgcgtg
    10621 gaatcctccc cttgcaacgg aatcctaccc tcatctgcag aatcctcccc tcgcattgcc
    10681 gcgcttcacg cggggaaccc tagcttacct cagttcccca ggatgtctgc gtggtaactg
    10741 gcggtgctgt gcttcttgct gcccgctaga gcaaggaggg atagatataa tgttgaagcc
    10801 tcgggtaccg agctcgaatt ctccaggcga tctgacggtt cactaaacga gctctgctta
    10861 tataggcctc ccaccgtaca cgcctacctc gacatacgtt ctctatcact gatagggagt
    10921 aaactcgaca tacgttctct atcactgata gggataaact cgacatacgt tctctatcac
    10981 tgatagggag taaactcgac atacgttctc tatcactgat agggagtaaa ctcgacatac
    11041 gttctctatc actgataggg agtaaactcg acatcgttct ctatcactga tagggagtaa
    11101 actcgacata cgttctctat cactgatagg gagtaaactc gtgtacatat tatgatatag
    11161 atacaacgta tgcaatggcc aatagccaat attgatttat gctatataac caatgactaa
    11221 tatggctaat tgccaatatt gattcaatgt atagatctca tggtgctgta tataaaacca
    11281 gtggttatat gtacagtagc tgtatataaa accagtggtt atatgtacag tagctgtata
    11341 taaaaccagt ggttatatgt acagtagtac gttagatctt ccatacctac cagttctgcg
    11401 cctgcagcaa tggcaacaac gttgcccgga tctgcga
//
