LOCUS       sequence-422692-        6064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fl. MBD2 2xStrep tagged in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Becker A, Allmann L, Hofstatter M, Casa V, Weber P, Lehmkuhl A, 
            Herce HD, Cardoso MC
  TITLE     Direct homo- and hetero-interactions of MeCP2 and MBD2.
  JOURNAL   PLoS One. 2013;8(1):e53730. doi: 10.1371/journal.pone.0053730. Epub 
            2013 Jan 15.
  PUBMED    23335972
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e53730. doi"; pages: " 
            10.1371/journal.pone.0053730. Epub 2013 Jan 15"
COMMENT     Sequence Label: pFB MBD2a-2-strep (pc1794)
FEATURES             Location/Qualifiers
     source          1..6064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      409..997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     898..917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1151..1168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  1302..1526
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1593..2126)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2296..2301,2302..2318,2319..2324))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2676..2767
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2692..2709
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4108..4131
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4171..4194
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    4345..4479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4382..4401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4436..4455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4508..4673
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4857..5311
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4944..4963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5153..5174
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5337..5441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5442..5510,5511..6064,1..238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5660..5679)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
       61 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
      121 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
      181 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
      241 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
      301 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
      361 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      421 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      481 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      541 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      601 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      661 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      721 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      781 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      841 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      901 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      961 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1021 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1081 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     1141 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     1201 ccttacgcat ctgtgcggta tttcacaccg catagaccag ccgcgtaacc tggcaaaatc
     1261 ggttacggtt gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt
     1321 cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag
     1381 ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc
     1441 taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg
     1501 ccaagggcat ggtaaagact atattcgcgg cgttgtgaca atttaccgaa caactccgcg
     1561 gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca
     1621 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca
     1681 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg
     1741 agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca
     1801 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg
     1861 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag
     1921 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca
     1981 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat
     2041 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc
     2101 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     2161 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc
     2221 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     2281 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc
     2341 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt
     2401 ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg
     2461 gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc
     2521 ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc
     2581 atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct
     2641 agtggttggc tacgtatact ccggaatatt aatagatcat ggagataatt aaaatgataa
     2701 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     2761 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatccc ggtccgaagc
     2821 gcgcggaatt caaaggccta cgtcgacgcc atgcgcgcgc acccgggggg aggccgctgc
     2881 tgcccggagc aggaggaggg ggagagcgcg gcgggcggca gcggcgctgg cggcgactcc
     2941 gccatagagc aggggggcca gggcagcgcg ctcgctccgt ccccggtgag cggcgtgcgc
     3001 agggaaggcg ctcggggcgg cggccgtggc cgggggcggt ggaagcaggc ggcccggggc
     3061 ggcggcgtct gtggccgtgg ccgtggccgt ggccggggtc ggggccgtgg ccggggccgg
     3121 ggccggggcc gcggccgtcc ccagagtggc ggcagcggcc ttggcggcga cggcggcggc
     3181 ggcgcgggcg gctgcggcgt cggcagcggt ggcggcgtcg ccccccggcg ggatcctgtc
     3241 cctttcccgt cggggagctc ggggccgggg cccaggggac cccgggccac ggagagcggg
     3301 aagaggatgg actgcccggc cctccccccc ggatggaaga aggaggaagt gatccgaaaa
     3361 tcagggctca gtgctggcaa gagcgatgtc tactacttca gtccaagtgg taagaagttc
     3421 agaagtaaac ctcagctggc aagatacctg ggaaatgctg ttgaccttag cagttttgac
     3481 ttcaggaccg gcaagatgat gcctagtaaa ttacagaaga acaagcagag actccggaat
     3541 gaccccctca atcagaacaa gggtaaacca gacctgaaca caacattgcc aattagacaa
     3601 actgcatcaa ttttcaagca accagtaacc aaattcacga accacccgag caataaggtg
     3661 aagtcagacc cccagcggat gaatgaacaa ccacgtcagc ttttctggga gaagaggcta
     3721 caaggactta gcgcatcaga tgtaacagaa caaattataa aaaccatgga gctacctaaa
     3781 ggtcttcaag gagtcggtcc aggtagcaat gacgagaccc ttctgtctgc tgtggccagt
     3841 gctttacaca caagctctgc gcccatcaca ggacaagtct ctgctgccgt ggaaaagaac
     3901 cctgctgttt ggcttaacac atctcaaccc ctctgcaaag ctttcattgt tacagatgaa
     3961 gacattagga aacaggaaga gcgagtccaa caagtacgca agaaactgga ggaggcactg
     4021 atggccgaca tcctgtcccg ggctgcggac acggaggaag tagacattga catggacgat
     4081 ggagatgagg cgtgcggccg cgccatgtgg agccatccac aattcgagaa gggtggcggg
     4141 tcgactggag gcggttcagg cgggggttca tggtcacatc cacaattcga gaagtaactc
     4201 gaggcatgcg gtaccaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     4261 tttgtagagg ttttacttgc tttaaaaaac cttccacacc tccccctgaa cctgaaacat
     4321 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     4381 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4441 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctagg
     4501 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     4561 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     4621 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
     4681 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
     4741 atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt
     4801 aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg aatgggacgc
     4861 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4921 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4981 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5041 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5101 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttaa tagtggactc
     5161 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5221 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5281 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5341 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5401 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5461 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5521 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5581 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5641 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5701 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5761 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5821 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5881 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5941 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6001 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6061 actg
//
