LOCUS       sequence-422855-        2639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses untagged ATG3 (Y209A) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Rao S, Skulsuppaisarn M, Strong LM, Ren X, Lazarou M, Hurley JH, 
            Hummer G
  TITLE     Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the 
            phagophore.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj8027. doi: 10.1126/sciadv.adj8027. 
            Epub 2024 Feb 7.
  PUBMED    38324698
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj8027. doi"; pages: " 
            10.1126/sciadv.adj8027. Epub 2024 Feb 7"
COMMENT     Sequence Label: pMX-IG-ATG3 (Y209A)
FEATURES             Location/Qualifiers
     source          1..2639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(329..1120,1121..1189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     952..971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1190..1294)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1446..1915
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2045..2062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2216..2639,1..165))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2296..2315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
       61 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      121 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      181 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      241 agattatcaa aaaggatctt cacctagatc cttttgcggc cggccgcaaa tcaatctaaa
      301 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      361 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      421 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      481 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      541 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
      601 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
      661 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
      721 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
      781 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
      841 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
      901 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
      961 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1021 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1081 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1141 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1201 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1261 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1321 cataatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa
     1381 ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg
     1441 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1501 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     1561 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
     1621 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     1681 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     1741 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
     1801 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     1861 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacatgc
     1921 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
     1981 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2041 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2101 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2161 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2221 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2281 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2341 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2401 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2461 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2521 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2581 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaac
//
