LOCUS       sequence-422899-        2564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses untagged ATG3 (K208D) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Rao S, Skulsuppaisarn M, Strong LM, Ren X, Lazarou M, Hurley JH, 
            Hummer G
  TITLE     Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the 
            phagophore.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj8027. doi: 10.1126/sciadv.adj8027. 
            Epub 2024 Feb 7.
  PUBMED    38324698
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj8027. doi"; pages: " 
            10.1126/sciadv.adj8027. Epub 2024 Feb 7"
COMMENT     Sequence Label: pMX-IG-ATG3 (K208D)
FEATURES             Location/Qualifiers
     source          1..2564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     473..492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     726..743
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1419..1523
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1524..1592,1593..2384)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1742..1761)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2548..2564,1..572)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
       61 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      661 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      721 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
      781 ccgcgcgttg gccgattcat taatgcaact atggccattt aatgtaaata cttaagaaaa
      841 aaaaccaaat taattttgat acatgctgca tgtgaaagac ccccgctgac gggtagtcaa
      901 tcactcagag gagaccctcc caaggaacag cgagaccaca agtcggatgc aactgcaaga
      961 gggtttattg gatacacggg tacccgggcg actcagtcaa tcggaggact ggcgccccga
     1021 gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga acagaagcga
     1081 gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc cttggggcac
     1141 cctggaaaca tctgatggtt ctctagaaac tgctgagggc tggaccgcat ctggggacca
     1201 tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca
     1261 tattcagctg ttccatctgt tcctgacctt gatctgaact tctctattct cagttatgta
     1321 tttttccatg ccttgcaaaa tggcgttact taagctagct tgccaaacct acaggtgggg
     1381 tctttcatta tgcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1441 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1501 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1561 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1621 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1681 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1741 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1801 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1861 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1921 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1981 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2041 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2101 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2161 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2221 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2281 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2341 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2401 actcatatat actttagatt gatttgcggc cggccgcaaa aggatctagg tgaagatcct
     2461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttc
//
