LOCUS       sequence-42302-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00418
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(518..617)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(633..650)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(633..649)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(642..664)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 cttataatgc caactttgta caagaaagtt ggcaattcat cagactccga ttccgattcg
      121 gactcttctt catcgtcatc atcgtcgtcg tcatcgtcgt cgtcgctgcc ggcggagcga
      181 tttagccgca gcatctcctg gaactggtgt acacggatct tttgcggcac gatctgcagc
      241 agaaactcca gattcttgtt cttattgacc acctgggaga gatgctcgta cttgagcgct
      301 tcgcccggtc gctggccgaa ttcctccgtg tatgccgcgc ccgccaaatg gcgaacgaat
      361 agctccgtgc acttggtcat caggaagagc acctcgttgg tgatcaagcc cgtgtccatg
      421 gagctcttca tgatcgttcg cacgcggctg aggggcagaa aggtttccgc ggtcggtgga
      481 cgctccacgg gtggttggct ccttggttcg cccatggtgc caactttttt gtacaaagtt
      541 ggcattataa aaaagcattg ctcatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      601 aataaaatca ttatttgggg cccgagctta agactggccg tcgttttaca acgtcgtgac
      661 tgggaaaaca
//
