LOCUS       sequence-423279-        4505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Streptomyces platensis LRD-modifying cytochrome P450 
            enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Lin GM, Voigt CA
  TITLE     Design of a redox-proficient Escherichia coli for screening 
            terpenoids and modifying cytochrome P450s
  JOURNAL   Nature Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature 
            Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5"
COMMENT     Sequence Label: pCYP34
FEATURES             Location/Qualifiers
     source          1..4505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(296..841)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(376..395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(930..1745)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(930..1745)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1653..1672
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1737..1756
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1922..1949
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1980..2007
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     primer_bind     complement(4417..4436)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttaggaa cacaccagta
     2101 agctatagga ggtatttaat gagtccgcca ccgagtggtg ccggggatgg tacatcctgc
     2161 gcgccccgtc ctccatcagg gcctctcccg attaaaggtc gtgatgtgac cgatgctagc
     2221 agccatccgg cgcctttacc gcgtgcgcgc cgatgtccgt tagacccacc agaggaatac
     2281 gccgccttgc gtcggaccga tccggtatcc cgtctggcat ttcccgatgg ttccgctggg
     2341 tggctggtga cccgttatga tgacgtgcga accgtgctga ccgatccgcg ctttagcgct
     2401 cggggagatc tcgttacctc gccggtagcc agccagttac ggcgccgtga tgcaccagcg
     2461 ccgggcatgt ttgctcgtat ggaccctccg gaccatactc gctatcgacg tctgttagcg
     2521 cgccactttc atgttcgtcg ggtccgcgca ctggtgcccg caatcgaacg catcgttgcc
     2581 gatcgcctcg atgcgcttcg tcgtgcaggt ccgccggccg atttagtgga aattttcgcc
     2641 ctgcctgtgc ccactctggt catttgcgaa ctgctgggtg tgccgtatga ggaccgagcg
     2701 gcgttccaga gctggacagc atctatggtt tcagtggaca gcacgcggga agaatctgat
     2761 gcggccgtgg ccgctctggc gggctatgtt cgctcactgg ttgtggcgaa acggggagtg
     2821 ccggcgcatg atcttcttgc cgacttagcc gcggatggtg aattgacgga tgaagaaacc
     2881 gccaacattg gccttagcgt acttgtggct ggccatgaaa ccaccgctaa tatgttgtcg
     2941 ttgggagctt tcgcactttt gcgccatccc gaggaactgg ctgccttccg tgccgatccg
     3001 ggtctgaccg agcaagctgt ggaagaactg ttgcgctatc tgaccatccc ccagttcggg
     3061 cgtgagcgcg ctgcactcga agatgtcgtg ctgggaggcc gcaccttggc cgccggtgaa
     3121 gttgtggtcg cttcactgtt gagcgcaaat cgcgacccgg ggcgctttga cgatccggat
     3181 accctggatc tgcgtcggcc atccgccggc cacctggcat ttggtcacgg aatccaccag
     3241 tgcataggac aacaactggc acgcgaacag ctcagagcgg gcttacgtgc tctgttcacc
     3301 caggggccca ctttacggtt ggcagttccc ccagaagaag taccaatgtg cgaggatagc
     3361 cttaattatg gtgttcgtcg cttacctgtc acgtggggta caggtcgcta ataaggatga
     3421 gctctacaaa taagcagagg tggttgtgtt gcgaaaaaaa aaaaaaaaca ccctaacggg
     3481 tgtttttttt tttttggtgt ccccacgtgt ggcgctggag accgtccaat ggcggcgcgc
     3541 catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
     3601 cagggtggtg aatatgagcc cgaaacgtcg tacccaggca gaacgtgcaa tggaaaccca
     3661 gggtaaactg attgcagcag cactgggtgt tctgcgtgaa aaaggttatg caggttttcg
     3721 tattgcagat gttccgggtg cagccggtgt tagccgtggt gcacagagcc atcattttcc
     3781 gaccaaactg gaactgctgc tggcaacctt tgaatggctg tatgagcaga ttaccgaacg
     3841 tagccgtgca cgtctggcaa aactgaaacc ggaagatgat gttattcagc agatgctgga
     3901 tgatgcagca gatttttttc tggatgatga ttttagcatc ggcctggatc tgattgttgc
     3961 agcagatcgt gatccggcac tgcgtgaagg tattctgcgt accgttgaac gtaatcgttt
     4021 tgttgttgaa gatatgtggc tgggtgtgct ggtgagccgt ggtctgagcc gtgatgatgc
     4081 cgaagatatt ctgtggctga tttttaacag cgttcgtggt ctgacagttc gtagcctgtg
     4141 gcagaaagat aaagaacgtt ttgaacgtgt gcgtaatagc accctggaaa ttgcacgtga
     4201 acgttatgca aaattcaaac gttgataagg atcctaattg gtaacgaatc agacaattga
     4261 cggctcgagg gagtagcata gggtttgcag aatccctgct tcgtccattt gacaggcaca
     4321 ttatgcatcg atgataagct gtcaaacatg agcagatcct ctacgccgga cgcatcgtgg
     4381 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     4441 gggaagatcg ggctcgccac ttcgggctca tgagcaaata ttttatctga ggtgcttcct
     4501 cgctc
//
