LOCUS       sequence-423332-       11730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR81. N-terminal NanoLuc 
            luciferase tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11730)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 11730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pNLF1-N_WDR81:1-1941
FEATURES             Location/Qualifiers
     source          1..11730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1065..1577
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(7498..7516)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    7548..7669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7559..7578)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7613..7632
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7914..8271
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7914..8105
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7937..7957)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8122..8257
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8302..9339
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             9318..9326
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    9363..9411
                     /note="synthetic polyadenylation signal"
     promoter        9567..9671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9672..9740,9741..10532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9890..9909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(10690..11278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10770..10789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11394..11677
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgcttc cgaattcatg gcacagggct
     1621 ctgggggtcg agaaggtgca ctccggacgc ctgctggtgg atggcacagc ccgccttccc
     1681 ctgacatgca ggaattgctt cgctccgtag aaagggatct ctctatagat ccacgccagc
     1741 ttgcgccggc acctggaggt acgcatgtcg tagccctggt accggccagg tggctcgcca
     1801 gtctgagaga tagacgactc ccattgggac cttgccccag ggctgaggga cttggtgagg
     1861 cggaggtgcg aaccttgctg cagaggtctg tccagaggct tcctgcgggt tggacaaggg
     1921 tagaggtgca cggtctccga aaacggcggt tgtcctatcc gctgggtggt ggcctccctt
     1981 ttgaggacgg aagttgcggc cctgaaactc tcacccggtt tatgcaagaa gtagccgcac
     2041 aaaactaccg aaatctgtgg aggcatgctt atcatacgta cgggcaaccg tattcccatt
     2101 cccctgcgcc gagcgcagta cccgcactgg atagtgtgcg acaagctctt cagcgggttt
     2161 atggttgttc ttttttgccc gttggcgaaa cgacgcaatg cccctcttac gctcgagagg
     2221 gaccttgtcc tccacgaggg agccctgcct gtccttcctt gttgcgggca gaggctctcc
     2281 tcgagagtcc ggagatgctg tatgttgtgc acccttacgt gcagtttagc ctgcatgatg
     2341 tggtgacctt cagcccagca aagttgacta atagtcaggc aaaagtgctc tttatcttgt
     2401 ttcgagtcct gcgagccatg gacgcttgcc accggcaagg tctcgcctgc ggtgcattgt
     2461 ctctttacca tattgccgtc gatgagaagc tctgcagtga gcttagattg gatctgagtg
     2521 cttacgaacg gcctgaggag gacgagaatg aggaggcccc agtggcgcga gacgaagcgg
     2581 gtatcgtatc ccaagaggag cagggtggcc aacccggtca acccacgggt caagaagagc
     2641 ttagatctct cgtgcttgac tgggtccacg gcagaatctc caacttccac tatttgatgc
     2701 aactcaacag actggcgggg cggaggcagg gtgacccgaa ttaccatcca gtcctgccgt
     2761 gggtggtaga tttcacaacc ccacatggtc ggttccgaga cctccggaaa tctaagttca
     2821 ggttgaacaa gggcgataag caactggact ttacctatga aatgactcgg caggcctttg
     2881 tagcgggcgg agcagggggg ggagagcctc cccacgtgcc gcatcatatc tcagatgtcc
     2941 tgtccgacat cacctactat gtgtacaaag ccagaaggac tcctcggtct gttttgtgtg
     3001 gacacgttcg cgcccaatgg gaaccccatg aatatccagc gtcaatggag cgaatgcaga
     3061 attggacgcc agacgaatgt attcctgagt tctacaccga cccgagtata tttcgatcta
     3121 ttcaccccga tatgcctgac ttggatgtgc ccgcatggtg ctcaagtagc caggagttcg
     3181 tagctgccca tcgcgccttg ttggaatcac gggaagtaag ccgggatctg caccattgga
     3241 ttgaccttac attcggatac aaattgcagg gtaaagaggc agttaaggaa aagaacgttt
     3301 gtcttcatct ggttgacgca cacacgcatc ttgcgtccta cggggttgtc caattgttcg
     3361 atcaacccca tccgcaaagg ctcgctggcg ccccagctct tgccccagaa ccgccgctta
     3421 tacctaaact tctggtgcag acgatacaag aaaccactgg tcgagaggat tttaccgaaa
     3481 atcctgggca attgcccaac ggagtcggca gaccagtatt ggaagcaacc ccctgtgaag
     3541 cttcttggac tagggataga cctgtggccg gtgaagatga cctggagcaa gccaccgaag
     3601 cactggatag tataagcctc gcgggaaagg ccggggacca gctcggaagc agctctcaag
     3661 ccagtcctgg cctcctcagc ttttcagtcg cgagtgcctc aagacctggg cggcgcaaca
     3721 aagccgccgg tgccgaccca ggcgaagggg aggaagggag gatattgctt ccagaagggt
     3781 tcaatcctat gcaggccttg gaggagctgg aaaaaacagg aaacttcctt gctaagggcc
     3841 ttggcggcct gctggaggtc ccagagcagc ctagggtaca gcccgcggta cccttgcagt
     3901 gcctgttgca ccgagacatg caggccttgg gtgtcctcct cgcggaaatg gtgtttgcga
     3961 cgcgagtccg aacgctccaa cctgacgctc ctttgtgggt gcgctttcag gcagtgcgcg
     4021 gactttgtac acggcacccg aaggaggtgc cagtttccct tcagcctgta ttggatacat
     4081 tgttgcagat gtccggaccg gaggttccca tgggtgccga gcggggtaag cttgaccaat
     4141 tgttcgagta tcgaccggtg agtcaaggac ttccaccccc atgcccatca cagctgctgt
     4201 ccccctttag ctcagtagtt cccttccctc cttatttccc ggcactgcat aggtttattc
     4261 tgctttatca ggctcgacgc gtcgaggatg aggcccaagg ccgggagttg gtattcgccc
     4321 tctggcaaca gctgggcgct gtgcttaaag acattacccc agagggactt gagatattgc
     4381 tgccctttgt cctcagcctt atgtccgagg aacataccgc ggtgtataca gcctggtacc
     4441 tctttgagcc tgttgcgaag gctctgggac ctaagaacgc aaataagtac ctcctgaaac
     4501 ccctcatagg agcttatgag tccccttgcc aattgcacgg gaggttttat ttgtatacag
     4561 attgttttgt ggcccagctc atggtaagat tgggcctgca ggcctttttg acgcacctgt
     4621 tgccgcacgt actccaagta ttggccggag ctgaagcatc acaggaagag tcaaaggatc
     4681 tggcgggagc tgctgaagaa gaagagtccg gtcttccagg ggcgggccca ggcagttgcg
     4741 catttggaga ggaaatcccg atggatggcg aacctccagc gtcttcaggt ctcggactcc
     4801 cagactatac aagcggggtt agcttccacg atcaggctga cctccccgag accgaagatt
     4861 tccaagctgg tctgtatgta accgaaagtc cacaaccgca agaagcggag gcagtgtcct
     4921 tgggtcgact cagcgacaaa tcttctacat ccgaaacttc tctgggcgag gagagggcac
     4981 cggatgaagg aggggcccct gttgacaaaa gctcacttag gtccggagat agtagtcaag
     5041 atctcaaaca gtctgaagga tccgaagaag aagaggagga agaggattcc tgtgttgtgc
     5101 tggaggagga agagggagaa caggaggaag ttaccggcgc aagtgaactg acacttagtg
     5161 atacggtgct ctcaatggag accgtagtcg ccggtggctc aggaggagac ggggaagagg
     5221 aagaggaggc cctgccagaa caatcagaag gtaaagaaca aaagatcctc cttgacaccg
     5281 cctgtaagat ggtccgctgg ctctccgcta agttgggacc aacggttgcg agtcggcacg
     5341 tggcgcggaa tctcctgaga ctgctcacaa gctgctacgt tggccctact aggcaacagt
     5401 tcacagtctc ctctggagaa tcccccccac tctccgctgg aaacatctac cagaagagac
     5461 cagtactggg tgacatcgtc tctgggccag tgttgagctg cttgttgcac atcgcacgat
     5521 tgtatggcga accggtactt acgtatcaat acctgccata tatttcttac ctcgtggccc
     5581 ccggctcagc ttctgggccc tcccgcctca atagccgaaa agaagcgggt ctgcttgctg
     5641 ctgtcacgtt gactcaaaaa attattgtat accttagcga cacgacactg atggacatct
     5701 tgccaagaat cagccatgag gtgctgcttc cagtcttgtc atttttgacc agtcttgtta
     5761 cggggtttcc gtccggtgcc caagcaagga ctatcctttg tgtaaaaaca atatcattga
     5821 tagcactcat ttgccttagg attggacagg agatggtaca acaacatttg tccgaacccg
     5881 tagctacttt tttccaagtc tttagtcagc tgcacgagct ccgccagcag gacttgaagt
     5941 tggatcctgc tggtagggga gagggccaac tgccccaggt cgttttcagc gatgggcaac
     6001 aaaggccagt cgacccggcc ctcctggatg aactccaaaa agttttcacc ctggaaatgg
     6061 cctacacgat atacgtgcct ttttcatgcc ttttgggtga tataatcaga aaaattatcc
     6121 ctaatcatga actcgttgga gaacttgctg ccctgtatct tgaaagcatt tcacccagtt
     6181 caaggaatcc agcaagtgtt gaaccgacaa tgcctggtac gggacccgag tgggatcctc
     6241 atggtggtgg ctgcccgcaa gacgacgggc attctggtac attcggtagc gtccttgtcg
     6301 gcaatcgcat tcagatacct aatgactccc gccccgagaa tcctggtcct ttgggaccga
     6361 tatcaggggt gggtggggga ggactcggaa gcggctccga cgacaatgcc ctcaaacagg
     6421 aactgccacg aagtgtccac ggcctgtcag gaaactggct ggcatactgg caatatgaaa
     6481 ttggtgttag tcagcaagat gcccacttcc atttccatca aatccgactt caatccttcc
     6541 ctggacactc aggtgcagtc aagtgtgtgg cgcctctttc cagcgaagac ttttttctct
     6601 caggatcaaa agatcggact gtacgcttgt ggccactcta caactatggt gatggcactt
     6661 ccgaaacagc tcctaggctg gtttatactc aacaccgaaa atcagtattt ttcgtaggac
     6721 agctcgaagc gccacagcac gttgtaagct gtgacggggc cgtgcacgta tgggatcctt
     6781 tcaccggtaa gaccctgcgc actgttgagc ctctcgactc tagggtaccc ttgactgccg
     6841 tcgcagttat gccggcccca catacgtcta tcaccatggc tagcagcgac tctacgttgc
     6901 ggtttgtaga ctgtcggaag cccggtctgc agcacgaatt cagattggga ggtggactta
     6961 accccgggct ggtcagagct ttggctatct ctccatcagg ccgctcagta gtggccggat
     7021 tttctagtgg gttcatggtc ctgctcgaca cgcggaccgg tctggttctt cggggctggc
     7081 ccgcgcatga aggtgacatt ttgcagatca aagccgttga aggaagtgtg ctggtatcat
     7141 catccagcga tcacagtctc acggtgtgga aagagctgga acagaaaccg acacatcatt
     7201 acaaatcagc ctcagatccg atccatacct tcgatttgta cgggagtgaa gttgtgacgg
     7261 gcacggtttc caacaagatc ggcgtttgta gtttgctcga accaccctca caggccacga
     7321 ccaagctcag tagtgagaat tttcgaggaa ccctcacaag tcttgccctg ctcccgacaa
     7381 aacgccatct gctcttggga tcagataatg gcgttattag gttgcttgca taataatcta
     7441 gatttgggcc caattcctgc aggattttgc ggccgcttgc tgagttggct gctgccaccg
     7501 ctgagcaata actagcataa ccccttggcc gcttcgagca gacatgataa gatacattga
     7561 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     7621 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     7681 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt taagcaagta
     7741 aaacctctac aaatgtggta aaatcgaatt ttaacaaaat attaacgctt acaatttcct
     7801 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gcggatctgc
     7861 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc
     7921 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     7981 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     8041 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     8101 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8161 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8221 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcgat
     8281 tcttctgaca ctagcgccac catgaagaag cccgaactca ccgctaccag cgttgaaaaa
     8341 tttctcatcg agaagttcga cagtgtgagc gacctgatgc agttgtcgga gggcgaagag
     8401 agccgagcct tcagcttcga tgtcggcgga cgcggctatg tactgcgggt gaatagctgc
     8461 gctgatggct tctacaaaga ccgctacgtg taccgccact tcgccagcgc tgcactaccc
     8521 atccccgaag tgttggacat cggcgagttc agcgagagcc tgacatactg catcagtaga
     8581 cgcgcccaag gcgttactct ccaagacctc cccgaaacag agctgcctgc tgtgttacag
     8641 cctgtcgccg aagctatgga tgctattgcc gccgccgacc tcagtcaaac cagcggcttc
     8701 ggcccattcg ggccccaagg catcggccag tacacaacct ggcgggattt catttgcgcc
     8761 attgctgatc cccatgtcta ccactggcag accgtgatgg acgacaccgt gtccgccagc
     8821 gtagctcaag ccctggacga actgatgctg tgggccgaag actgtcccga ggtgcgccac
     8881 ctcgtccatg ccgacttcgg cagcaacaac gtcctgaccg acaacggccg catcaccgcc
     8941 gtaatcgact ggtccgaagc tatgttcggg gacagtcagt acgaggtggc caacatcttc
     9001 ttctggcggc cctggctggc ttgcatggag cagcagactc gctacttcga gcgccggcat
     9061 cccgagctgg ccggcagccc tcgtctgcga gcctacatgc tgcgcatcgg cctggatcag
     9121 ctctaccaga gcctcgtgga cggcaacttc gacgatgctg cctgggctca aggccgctgc
     9181 gatgccatcg tccgcagcgg ggccggcacc gtcggtcgca cacaaatcgc tcgccggagc
     9241 gcagccgtat ggaccgacgg ctgcgtcgag gtgctggccg acagcggcaa ccgccggccc
     9301 agtacacgac cgcgcgctaa ggaggtaggt cgagtttaga ctctagccat cacgatggcc
     9361 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     9421 cgataaggat cctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     9481 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtaatggtt tcttagacgt
     9541 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9601 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9661 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9721 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9781 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9841 gttttcgccc cgaagaacgt tttccaatga tgagcacttt caaagttctg ctatgtggcg
     9901 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9961 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10021 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10081 tgacaactat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10141 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10201 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10261 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10321 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10381 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10441 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10501 agataggtgc ctcactgatt aagcattggt aattcgaaat gaccgaccaa gcgacgccca
    10561 accggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10621 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10681 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10741 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10801 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10861 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10921 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10981 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    11041 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11101 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    11161 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11221 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatttcaaga
    11281 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    11341 gattttggtc atgagattat caaaaaggat cttcacctag atccttttat agtccggaaa
    11401 tacaggaacg cacgctggat ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc
    11461 ttcagtggat taagtggggg taatgtggcc tgtaccctct ggttgcatag gtattcatac
    11521 ggttaaaatt tatcaggcgc gattgcggca gtttttcggg tggtttgttg ccatttttac
    11581 ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt aagggattat
    11641 ggtaaatcca cttactgtct gccctcgtag ccatcgagat aaaccgcagt actccggcca
    11701 cgatgcgtcc ggcgtagagg atcgagatct
//
