LOCUS       sequence-423356-        5942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human RBBP5. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_RBBP5:1-538
FEATURES             Location/Qualifiers
     source          1..5942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3005..3126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3016..3035)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3241..3274)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3284..3331
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3363..4157
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3417..3436)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4027..4046
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4315..4903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4395..4414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5029..5077
                     /note="synthetic polyadenylation signal"
     CDS             complement(5101..5499)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5496..5505
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5530..5887)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5544..5679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5696..5887
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5844..5864
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatgaacct cgagttgctg
     1261 gagtcctttg ggcagaacta tccagaggaa gctgatggaa ctttggattg tatcagcatg
     1321 gctttgactt gcacctttaa caggtggggc acactgcttg cagttggctg taatgatggc
     1381 cgaattgtca tctgggattt cttgacaaga ggcattgcta aaataattag tgcacacatc
     1441 catccagtgt gttctttatg ctggagccga gatggtcata aactcgtgag tgcttccact
     1501 gataacatag tgtcacagtg ggatgttctt tcaggcgact gtgaccagag gtttcgattc
     1561 ccttcaccca tcttaaaagt ccaatatcat ccacgagatc agaacaaggt tctcgtgtgt
     1621 cccatgaaat ctgctcctgt catgttgacc ctttcagatt ccaaacatgt tgttctgcca
     1681 gtggacgatg actccgattt gaacgttgtg gcatcttttg ataggcgagg ggaatatatt
     1741 tatacgggaa acgcaaaagg caagattttg gtcctaaaaa cagattctca ggatcttgtt
     1801 gcttccttca gagtgacaac tggaacaagc aataccacag ccattaagtc aattgagttt
     1861 gcccggaagg ggagttgctt tttaattaac acggcagatc gaataatcag agtttatgat
     1921 ggcagagaaa tcttaacatg tggaagagat ggagagcctg aacctatgca gaaattgcag
     1981 gatttggtga ataggacccc atggaagaaa tgttgtttct ctggggatgg ggaatacatc
     2041 gtggcaggtt ctgcccggca gcatgccctg tacatctggg agaagagcat tggcaacctg
     2101 gtgaagattc tccatgggac gagaggagaa ctcctcttgg atgtagcttg gcatcctgtt
     2161 cgacccatca tagcatccat ttccagtgga gtggtatcta tctgggcaca gaatcaagta
     2221 gaaaactgga gtgcatttgc accagacttc aaagaattgg atgaaaatgt agaatacgaa
     2281 gaaagggaat cagagtttga tattgaagat gaagataaga gtgagcctga gcagacaggg
     2341 gctgatgctg cagaagatga ggaagtggat gtcaccagcg tggaccctat tgctgccttc
     2401 tgtagcagtg atgaagagct ggaagattca aaggctctat tgtatttacc cattgcccct
     2461 gaggtagaag acccagaaga aaatccttac ggccccccac cggatgcagt ccaaacctcc
     2521 ttgatggatg aaggggctag ttcagagaag aagaggcagt cctcagcaga tgggtcccag
     2581 ccacctaaga agaaacccaa aacaaccaat atagaacttc aaggagtacc aaatgatgaa
     2641 gtccatccac tactgggtgt gaagggggat ggcaaatcca agaagaagca agcaggccgg
     2701 cctaaaggat caaaaggtaa agagaaagat tctccattta aaccgaaact ctacaaaggg
     2761 gacagaggtt tacctctgga aggatcagcg aagggtaaag tgcaggcgga actcagccag
     2821 cccttgacag caggaggagc aatctcagaa ctgttaggct cgagcggtgg gagctccggt
     2881 gtgagcggct ggcggctgtt caagaagatt agctaagttt aaacggccgc gactctagac
     2941 tgcaggcatg caagctgatt tggctgctgc caccgctgag caaggccgct tcgagcagac
     3001 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     3061 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     3121 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     3181 gtttttttaa gcaagtaaaa cctctacaaa tgtggtaaaa tcttaattaa gacttctaat
     3241 ataacttcgt atagcataca ttatacgaag ttatggttcc actgttgaca attaatcatc
     3301 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaaccc aggaggcaga
     3361 tcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3421 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3481 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3541 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3601 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3661 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     3721 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     3781 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3841 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     3901 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3961 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4021 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4081 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4141 ttgacgagtt cttctgattc gaaatgaccg accaagcgac gcccaaccgg tatcagctca
     4201 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4261 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4321 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4381 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4441 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4501 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4561 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4621 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4681 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4741 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4801 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4861 tgtttgcaag cagcagatta cgcgcagaaa aaaaggattt caagaagatc ctttgatctt
     4921 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4981 attatcaaaa aggatcttca cctagatcct tttatagtcc ggaatcgtca cacaaaaaac
     5041 caacacacag atgtaatgaa aataaagata ttttattgcg gccatcgtga tggctagagt
     5101 ttagccctcc cacacataac cagagggcag caattcacga atcccaactg ccgtcggctg
     5161 tccatcactg tccttcacta tggctttgat cccaggatgc agatcgagaa gcacctgtcg
     5221 gcaccgtccg caggggctca agatgcccct gttctcattt ccgatcgcga cgatacaagt
     5281 caggttgcca gctgccgcag cagcagcagt gcccagcacc acgagttctg cacaaggtcc
     5341 cccagtaaaa tgatatacat tgacaccagt gaagatgcgg ccgtcgctag agagagctgc
     5401 gctggcgacg ctgtagtctt cagagatggg gatgctgttg attgtagccg ttgctctttc
     5461 aatgagggtg gattcttctt gagacaaagg cttggccatg gtggcgctag tgtcagaaga
     5521 atcgagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     5581 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     5641 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     5701 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     5761 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     5821 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     5881 gcctcagaag gtacctaacc aagttcctct ttcagaggtt atttcaggcc atggtgctgc
     5941 gc
//
