LOCUS       sequence-42355-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SETD1A
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             450..467
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(520..538)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      534..581
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(705..724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctctgaata ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac
       61 agcagcggca gagaaaactt gtatttccag ggcggacaga ttccactgaa ggaagtgact
      121 tttgcaaggc tgaatgacaa cgtgcgggag accttcctga aggatatgtg ccgtaagtac
      181 ggtgaggtgg aagaggtaga gatcctcctt cacccccgta cgcgcaagca cctgggcctg
      241 gcccgtgtgc tcttcaccag cactcggggc gccaaggaaa cggtcaaaaa cctccacctt
      301 acctccgtca tgggcaacat catccatgcc cagcttgaca tcaaaggaca acaacgaatg
      361 aaatactatg aactaattgt caatggctcc tacacccctc agactgtgcc cactgggggc
      421 tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg
      481 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      541 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      601 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      781 tttagggttc cgatttagtg ctttacggca c
//
