LOCUS       sequence-423554-        8428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852
            in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Grubb T, Maganti S, Krill-Burger JM, Fraser C, Stransky L, 
            Radivoyevitch T, Sarosiek KA, Vazquez F, Kaelin WG, Chakraborty AA
  TITLE     A Mesenchymal Tumor Cell State Confers Increased Dependency on the 
            BCL-XL Antiapoptotic Protein in Kidney Cancer.
  JOURNAL   Clin Cancer Res. 2022 Nov 1;28(21):4689-4701. doi: 
            10.1158/1078-0432.CCR-22-0669.
  PUBMED    35776130
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/1078-0432";
            journalName: "Clin Cancer Res"; date: "2022-11-1- 1"; volume: "28"; 
            issue: "21"; pages: "4689-4701"
COMMENT     Sequence Label: pLX304-BCL-XL-F97W
FEATURES             Location/Qualifiers
     source          1..8428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1327..1351)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1353..1394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1436..2024
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1489..1509)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1907..1918)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2096..2329
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2407..2528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2444..2463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2498..2517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2568..2703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2723..2742)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2724..2742)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2752..2769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2752..2768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2761..2783)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2910..3365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2997..3016)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3207..3228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3391..3495
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3496..3564,3565..4356)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3714..3733)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4527..5115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5016..5035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5269..5286
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5403..5424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5439..5444,5445..5462,5463..5469)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5477..5493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5482..5504
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5501..5517
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5501..5517
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5536..5556
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5538..5556
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5584..5810
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5811..5991
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6038..6163
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6656..6889
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7074..7118
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7293..7793
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7712..7730
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7805..8200
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8260..8377
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagcaggct accatgtctc agagcaaccg ggagctggtg gttgactttc
      661 tctcctacaa gctttcccag aaaggataca gctggagtca gtttagtgat gtggaagaga
      721 acaggactga ggccccagaa gggactgaat cggagatgga gacccccagt gccatcaatg
      781 gcaacccatc ctggcacctg gcagacagcc ccgcggtgaa tggagccact ggccacagca
      841 gcagtttgga tgcccgggag gtgatcccca tggcagcagt aaagcaagcg ctgagggagg
      901 caggcgacga gtgggaactg cggtaccggc gggcattcag tgacctgaca tcccagctcc
      961 acatcacccc agggacagca tatcagagct ttgaacaggt agtgaatgaa ctcttccggg
     1021 atggggtaaa ctggggtcgc attgtggcct ttttctcctt cggcggggca ctgtgcgtgg
     1081 aaagcgtaga caaggagatg caggtattgg tgagtcggat cgcagcttgg atggccactt
     1141 acctgaatga ccacctagag ccttggatcc aggagaacgg cggctgggat acttttgtgg
     1201 aactctatgg gaacaatgca gcagccgaga gccgaaaggg ccaggaacgc ttcaaccgct
     1261 ggttcctgac gggcatgact gtggccggcg tggttctgct gggaagttta ttttcaagaa
     1321 agtgcgaccc agctttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
     1381 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
     1441 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1501 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     1561 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     1621 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     1681 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     1741 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     1801 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     1861 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     1921 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     1981 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     2041 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     2101 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     2161 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2221 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2281 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     2341 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     2401 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2521 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     2581 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2641 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2701 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     2761 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     2821 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     2881 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     2941 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3001 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3061 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3121 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3181 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3241 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3301 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     3361 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3421 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3481 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3541 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3601 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3661 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3721 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3781 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3841 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3901 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3961 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4021 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4081 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4141 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4201 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4261 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4321 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4381 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4441 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4501 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4561 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4621 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4681 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4741 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4801 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4861 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4921 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4981 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5041 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5101 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5161 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5221 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5281 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5341 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5401 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     5461 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     5521 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     5581 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     5641 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     5701 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     5761 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     5821 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5881 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5941 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     6001 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     6061 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     6121 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     6181 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     6241 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     6301 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     6361 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     6421 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     6481 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     6541 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     6601 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     6661 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     6721 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     6781 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     6841 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     6901 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     6961 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     7021 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     7081 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     7141 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     7201 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     7261 ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc agccctgggt
     7321 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     7381 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     7441 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     7501 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     7561 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     7621 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     7681 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     7741 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggtac
     7801 caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     7861 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     7921 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     7981 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     8041 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     8101 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     8161 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc agtagtaaga
     8221 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     8281 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     8341 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     8401 agatccactt tggcgccggc tcgagggg
//
