LOCUS       sequence-424314-       10012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human P53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Wu YQ, Zhang CS, Xiong J, Cai DQ, Wang CZ, Wang Y, Liu YH, Wang Y, 
            Li Y, Wu J, Wu J, Lan B, Wang X, Chen S, Cao X, Wei X, Hu HH, Guo H,
            Yu Y, Ghafoor A, Xie C, Wu Y, Xu Z, Zhang C, Zhu M, Huang X, Sun X, 
            Lin SY, Piao HL, Zhou J, Lin SC
  TITLE     Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine
            synthesis to p53 activation to control cell fate.
  JOURNAL   Cell Res. 2023 Nov;33(11):835-850. doi: 10.1038/s41422-023-00874-4. 
            Epub 2023 Sep 19.
  PUBMED    37726403
REFERENCE   2  (bases 1 to 10012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-023-00874-4"; journalName: "Cell Res"; date: 
            "2023-11"; volume: "33"; issue: "11"; pages: "835-850"
COMMENT     Sequence Label: pBOBI TP53 S46A
FEATURES             Location/Qualifiers
     source          1..10012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..387
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             405..585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    632..757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1250..1483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1668..1712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1964..2080
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2103..2406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2407..2610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2607..2631
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2718..3899
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     APDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     intron          3986..4215
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4198..4217
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4392..4980
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4445..4465)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4863..4874)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5387..5567
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5593..5610)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5599..5823
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5869..6297
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5956..5975)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6166..6187
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6306..6326)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6311..6640
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6491..6626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6553..6572
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6688..6735
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6754..7128
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7258..7379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7295..7314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7349..7368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7428..7444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7428..7444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7441..7463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7452..7468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7476..7482,7483..7500,7501..7506))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7521..7542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7659..7676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7830..8418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7910..7929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8589..9380,9381..9449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9212..9231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9450..9554)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9629..9648)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9820..10012,1..187)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
       61 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      121 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      181 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      241 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      301 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      361 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      421 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      481 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      541 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      601 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      661 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      721 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      781 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      841 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      901 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      961 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1021 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1081 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1141 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1201 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1261 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1321 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1381 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1441 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1501 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1561 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1621 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1681 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1741 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1801 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1861 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1921 agagagacag agacagatcc attcgattag tgaacggatc aacttttaaa agaaaagggg
     1981 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2041 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc
     2101 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2161 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2221 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2281 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2341 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2401 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2461 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2521 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2581 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2641 cgccatccac gctgttttga cctccataga agacaccgac tgagctcctt aaggttaact
     2701 ctagagaatt cggatccatg gaggagccgc agtcagatcc tagcgtcgag ccccctctga
     2761 gtcaggaaac attttcagac ctatggaaac tacttcctga aaacaacgtt ctgtccccct
     2821 tgccgtccca agcaatggat gatttgatgc tggccccgga cgatattgaa caatggttca
     2881 ctgaagaccc aggtccagat gaagctccca gaatgccaga ggctgctccc cccgtggccc
     2941 ctgcaccagc agctcctaca ccggcggccc ctgcaccagc cccctcctgg cccctgtcat
     3001 cttctgtccc ttcccagaaa acctaccagg gcagctacgg tttccgtctg ggcttcttgc
     3061 attctgggac agccaagtct gtgacttgca cgtactcccc tgccctcaac aagatgtttt
     3121 gccaactggc caagacctgc cctgtgcagc tgtgggttga ttccacaccc ccgcccggca
     3181 cccgcgtccg cgccatggcc atctacaagc agtcacagca catgacggag gttgtgaggc
     3241 gctgccccca ccatgagcgc tgctcagata gcgatggtct ggcccctcct cagcatctta
     3301 tccgagtgga aggaaatttg cgtgtggagt atttggatga cagaaacact tttcgacata
     3361 gtgtggtggt gccctatgag ccgcctgagg ttggctctga ctgtaccacc atccactaca
     3421 actacatgtg taacagttcc tgcatgggcg gcatgaaccg gaggcccatc ctcaccatca
     3481 tcacactgga agactccagt ggtaatctac tgggacggaa cagctttgag gtgcgtgttt
     3541 gtgcctgtcc tgggagagac cggcgcacag aggaggagaa tctccgcaag aaaggggagc
     3601 ctcaccacga gctgccccca gggagcacta agcgagcact gcccaacaac accagctcct
     3661 ctccccagcc aaagaagaaa ccactggatg gagaatattt cacccttcag atccgtgggc
     3721 gtgagcgctt cgagatgttc cgagagctga atgaggcctt ggaactcaag gatgcccagg
     3781 ctgggaagga gccagggggg agcagggctc actccagcca cctgaagtcc aaaaagggtc
     3841 agtctacctc ccgccataaa aaactcatgt tcaagacaga agggcctgac tcagactgac
     3901 tcgagccatg gaagcttgat atctaactga ctgaaccggt ggtaccgagg aattaattcg
     3961 ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct
     4021 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg
     4081 atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc
     4141 ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg
     4201 cctttctctc cacaggtgtc cactcccagg tccaactgca ggtcgagcat gcatctaggg
     4261 cggccaattc cgccgatcct ggaaaaacat ggagcaatca caagtagcaa tacagcagct
     4321 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     4381 acacctcaga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4441 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4501 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4561 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4621 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4681 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4741 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4801 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4861 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4921 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4981 ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     5041 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5101 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     5161 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     5221 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     5281 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     5341 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     5401 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5461 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5521 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg ccgctttaaa
     5581 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5641 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5701 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5761 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     5821 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     5881 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5941 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6001 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6061 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6121 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6181 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6241 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     6301 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6361 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6421 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6541 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     6601 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     6661 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     6721 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     6781 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     6841 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     6901 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     6961 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     7021 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     7081 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     7141 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     7201 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     7261 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7321 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7381 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     7441 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7501 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7561 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7621 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7681 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7741 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7801 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7861 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7921 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7981 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8041 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8101 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8161 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8221 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8281 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8341 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8401 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8461 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8521 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8581 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8641 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8701 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8761 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8821 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8881 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8941 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9001 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9061 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9121 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9181 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9241 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9301 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9361 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9421 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9481 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9541 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     9601 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     9661 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     9721 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     9781 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     9841 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     9901 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     9961 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cg
//
